| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05555-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08969 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47949-0.1mg | |||
| IHC-Prediluted | NBP2-48190 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73605 | |||
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0011.jpg "Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.")
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in PC-12.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0008.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in IMR-32.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0009.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in Raw 264.7.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0010.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10 Analysis of YWHAG expression in K-562.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0012.jpg)
![ELISA: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Sandwich-ELISA-H00007532-M02-img0007.jpg)
![Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - SPRY1 monoclonal antibody (M01), clone 3H4 Analysis of SPRY1 expression in HepG2.](http://images.novusbio.com/fullsize/SPRY1-Antibody-3H4-Western-Blot-H00010252-M01-img0010.jpg "Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - SPRY1 monoclonal antibody (M01), clone 3H4 Analysis of SPRY1 expression in HepG2.")
![Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SPRY1-Antibody-3H4-Immunohistochemistry-Paraffin-H00010252-M01-img0008.jpg)
![Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.Lane 1: SPRY1 transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPRY1-Antibody-3H4-Western-Blot-H00010252-M01-img0011.jpg)
![ELISA: SPRY1 Antibody (3H4) [H00010252-M01] - Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPRY1-Antibody-3H4-ELISA-H00010252-M01-img0012.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NBP3-05555-img0002.jpg "Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.")
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555] - Immunofluorescent analysis of rat brain section stained with Tyrosine Hydroxylase Antibody at a dilution of 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Tyrosine Hydroxylase Antibody stains the dopaminergic TH-positive neurons and their processes of the substantia nigra.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05555-img0001.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-Paraffin-NBP3-07898-img0002.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).")
![Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-NBP3-07898-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689] - Analysis of anti-Adenosine A3 Receptor / ADORA3 antibody with human tonsil, squamous epithelium at dilution 20-30 ug/ml.](http://images.novusbio.com/fullsize/Adenosine-A3-R-Antibody-Immunohistochemistry-Paraffin-NLS689-img0003.jpg)
![Immunohistochemistry: Adenosine A3 R Antibody [NLS689] - si-CD73 inhibits HNSCC tumor growth & suppress EMT in vivo(A) Tumor growth curve of siRNA-CD73 mice & control mice. Data represent the mean ± SEM. of eight mice in each group. *P < 0.05, **P < 0.01, ***P < 0.001 by the Student's t-test; (B) Dissected tumors were photographed. The tumor volume & weight were measured. ***P < 0.001 by the Student's t-test; (C) Representative images of immunohistochemical analysis of CD73, p-EGFR, p-Src, A3R, Slug, E-cad, N-cad & Vimentin in tumors, (Scale bars = 100 μm). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls689_rabbit-adenosine-a3-r-pab-310202415392530.jpg)
![Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects GRIN2A protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NMDAR2A antibody dilution: 1:500.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Immunohistochemistry-Paraffin-NBP2-19551-img0003.jpg "Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects GRIN2A protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NMDAR2A antibody dilution: 1:500.")
![Western Blot: NMDAR2A Antibody [NBP2-19551] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Western-Blot-NBP2-19551-img0001.jpg)
![Western Blot: NMDAR2A Antibody [NBP2-19551] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (NBP2-19551) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19551_rabbit-polyclonal-nmdar2a-antibody-91020242075985.jpg)
![Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NMDAR2A stained by NMDAR2A antibody (NBP2-19551) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19551_rabbit-polyclonal-nmdar2a-antibody-910202420104485.jpg)
![NMDAR2A antibody detects NMDAR2A protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: NMDAR2A protein stained by NMDAR2A antibody (NBP2-19551) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19551_rabbit-polyclonal-nmdar2a-antibody-immunocytochemistry-immunofluorescence-2611202518372411.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).](http://images.novusbio.com/fullsize/Vimentin-Antibody-LN-6-Immunohistochemistry-Paraffin-NBP2-44832-img0002.jpg "Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).")
![Western Blot: VAChT/SLC18A3 Antibody [NB100-91348] - Rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Western-Blot-NB100-91348-img0032.jpg "Western Blot: VAChT/SLC18A3 Antibody [NB100-91348] - Rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - Rat spinal cord. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0031.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0015.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0016.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0018.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0026.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0028.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0029.jpg)
![Immunohistochemistry-Frozen: LRRK2 Antibody Pack [NB100-938] - Staining of muscle sections as described in PMID 24312256. Image from verified customer review. LRRK2 Antibody [NB300-268]](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Immunohistochemistry-Frozen-NB100-938-img0017.jpg "Immunohistochemistry-Frozen: LRRK2 Antibody Pack [NB100-938] - Staining of muscle sections as described in PMID 24312256. Image from verified customer review. LRRK2 Antibody [NB300-268]")
![Western Blot: LRRK2 Antibody Pack [NB100-938] - LRRK2 N-terminal sequences show increased aggregation. Western blots of cell lysates of SH-SY5Y cells transfected with indicated LRRK2 construct showed equal expression of the LRRK2 protein with anti-HA (top panel) or anti-C-terminal- (bottom panel) LRRK2 antibody. Equal lysate loading is indicated by Beta-actin loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045149) licensed under a CC-BY license. LRRK2 Antibody [NB300-268]](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Western-Blot-NB100-938-img0016.jpg)
![Immunohistochemistry: LRRK2 Antibody Pack [NB100-938] - Staining of neurons and glia using NB110-55289 at 2.5ug/ml. Mouse brain, 40x.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Immunohistochemistry-NB100-938-img0013.jpg)
![Western Blot: LRRK2 Antibody Pack [NB100-938] - Detection of murine LRRK2 in mouse brain lysate using NB110-55289.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Western-Blot-NB100-938-img0014.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0019.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-NBP2-02577-img0013.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0015.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0011.jpg)
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0016.jpg)
![Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Western-Blot-NBP2-02577-img0017.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Western-Blot-NBP2-02577-img0018.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Immunohistochemistry-Paraffin-NBP2-02577-img0014.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0009.jpg)
![Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-1D5-Flow-Cytometry-NBP2-02577-img0012.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113530.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114766.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613638.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114752.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241611472.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416114785.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-31020241613635.jpg)
![Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02577_mouse-profilin-1-mab-oti1d5-310202416113538.jpg)
![Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382] - Staining of human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Paraffin-NB100-41382-img0006.jpg "Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382] - Staining of human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.")
![Flow Cytometry: 5-HT3A Antibody [NB100-41382] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Flow-Cytometry-NB100-41382-img0008.jpg)
![Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382] - Analysis of 5-HT3A in C57BL/6 mouse retina section using anti-5-HT3A antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Frozen-NB100-41382-img0003.jpg)
![Western Blot: GABA-A R alpha 6 Antibody [NB300-196] - Analysis of mouse forebrain lysates from Wild Type (Control) and a6-knockout (a6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a6-knockout animals.](http://images.novusbio.com/fullsize/GABA-A-Receptor-alpha-6-Antibody-Western-Blot-NB300-196-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-A R alpha 6 Antibody [NB300-196] - Analysis of mouse forebrain lysates from Wild Type (Control) and a6-knockout (a6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a6-knockout animals.")
![<b>Genetic Strategies Validation. </b>Western Blot: GABA A Receptor alpha 6 Antibody [NB300-196] - Detection of GABA-A alpha 6 receptor in wildtype cerebellum (5-7 ug/lane).](http://images.novusbio.com/fullsize/GABA-A-Receptor-alpha-6-Antibody-Western-Blot-NB300-196-img0001.jpg)