5-HT3A Antibody

Images

 
Western Blot: 5-HT3A Antibody [NB100-41382] - Staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382] - Analysis of 5-HT3A in C57BL/6 mouse retina section using anti-5-HT3A antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

5-HT3A Antibody Summary

Immunogen
Peptide with sequence C-GPQDFEKSPRDR corresponding to internal region according to NP_998786.2, NP_000860.2, NP_001155244.1.
Specificity
This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HTR3A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
ICC/IF successful usage for rat neurons is reported in scientific literature (PMID: 19344720). WB: Approx. 55 kDa band observed in human colon lysates (calculated MW of 55.3 kDa band according to NP_000860.1. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Small Intestine.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
5-HT3A Lysate (NBL1-11779)
Reviewed Applications
Read 1 Review rated 4
using
NB100-41382 in the following applications:

Publications
Read Publications using NB100-41382.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-HT3A Antibody

  • 5-HT-3,5-HT3-A
  • 5HT3A
  • 5-HT3A
  • 5-HT3R5-hydroxytryptamine receptor 3A
  • 5-hydroxytryptamine (serotonin) receptor 3A
  • 5-hydroxytryptamine receptor 3,5-HT3A5HT3R
  • HTR35HT3 serotonin receptor
  • HTR3A
  • Serotonin receptor 3A
  • Serotonin-gated ion channel receptor

Background

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-HT3A Antibody (NB100-41382)(3)

Review for 5-HT3A Antibody (NB100-41382) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NB100-41382:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen 5-HT3A NB100-41382
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reviewed by:
Renee Ryals
IHC-Fr Mouse 03/03/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedC57BL/6 mouse retina
SpeciesMouse
LotP1N110712

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-HT3A Antibody (NB100-41382) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Renee Ryals
03/03/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol HTR3A
Entrez
Uniprot