| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03072-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61122-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99436-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26216-50ul | |||
![Western Blot: TOMM22 Antibody (6R1L7) [NBP3-16856] - Western blot analysis of extracts of various cell lines, using TOMM22 Rabbit mAb (NBP3-16856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TOMM22-Antibody-6R1L7-Western-Blot-NBP3-16856-img0002.jpg "Western Blot: TOMM22 Antibody (6R1L7) [NBP3-16856] - Western blot analysis of extracts of various cell lines, using TOMM22 Rabbit mAb (NBP3-16856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: TOMM22 Antibody (6R1L7) [NBP3-16856] - Immunohistochemistry of paraffin-embedded human colon using TOMM22 Rabbit mAb (NBP3-16856) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TOMM22-Antibody-6R1L7-Immunohistochemistry-Paraffin-NBP3-16856-img0001.jpg)
![Western Blot: TOMM22 Antibody (6R1L7) [NBP3-16856] - Western blot analysis of extracts of various cell lines, using TOMM22 Rabbit mAb (NBP3-16856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TOMM22-Antibody-6R1L7-Western-Blot-NBP3-16856-img0003.jpg)
![Immunoprecipitation: TOMM22 Antibody (6R1L7) [NBP3-16856] - Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3 ug TOMM22 antibody . Western blot was performed from the immunoprecipitate using TOMM22 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16856_rabbit-tomm22-mab-6r1l7-52202511402781.jpg)
![Western Blot: ERP29 Antibody [NBP3-03072] - Analysis of extracts of various cell lines, using ERP29 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/ERP29-Antibody-Western-Blot-NBP3-03072-img0002.jpg "Western Blot: ERP29 Antibody [NBP3-03072] - Analysis of extracts of various cell lines, using ERP29 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![<b>Genetic Strategies Validation. </b>Western Blot: ERP29 Antibody [NBP3-03072] - Analysis of extracts from normal (control) and ERP29 knockout (KO) 293T cells, using ERP29 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBS.](http://images.novusbio.com/fullsize/ERP29-Antibody-Knockout-Validated-NBP3-03072-img0001.jpg)
![Western Blot: ERP29 Antibody - BSA Free [NBP3-03072] - Western blot analysis of various lysates using ERP29 Rabbit pAb (A7959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-03072_rabbit-polyclonal-erp29-antibody-52202519294584.jpg)
![SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.](http://images.novusbio.com/fullsize/Recombinant-Human-HSPA8-HSC71-Hsc70-Protein-SDS-Page-NBP1-30278-img0001.jpg "SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.")
![Western Blot: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - The Recombinant Human HSP90 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Western-Blot-NBP1-04301-img0004.jpg "Western Blot: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - The Recombinant Human HSP90 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - ICC/IF analysis of HSP90 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HSP90 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-04301-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Immunohistochemistry-Paraffin-NBP1-04301-img0002.jpg)
![Flow Cytometry: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with NBP1-04301 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Flow-Cytometry-NBP1-04301-img0008.jpg)
![Western Blot: RAB9A Antibody (7O1G4) [NBP3-16506] - Western blot analysis of extracts of various cell lines, using RAB9A Rabbit mAb (NBP3-16506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RAB9A-Antibody-7O1G4-Western-Blot-NBP3-16506-img0003.jpg "Western Blot: RAB9A Antibody (7O1G4) [NBP3-16506] - Western blot analysis of extracts of various cell lines, using RAB9A Rabbit mAb (NBP3-16506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: RAB9A Antibody (7O1G4) [NBP3-16506] - Immunofluorescence analysis of NIH-3T3 cells using RAB9A Rabbit mAb (NBP3-16506) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RAB9A-Antibody-7O1G4-Immunocytochemistry-Immunofluorescence-NBP3-16506-img0002.jpg)
![Immunohistochemistry-Paraffin: RAB9A Antibody (7O1G4) [NBP3-16506] - Immunohistochemistry of paraffin-embedded rat testis using RAB9A Rabbit mAb (NBP3-16506) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RAB9A-Antibody-7O1G4-Immunohistochemistry-Paraffin-NBP3-16506-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (7O1G4) [NBP3-16506] - Confocal imaging of NIH/3T3 cells using RAB9A Rabbit mAb (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16506_rabbit-rab9a-mab-7o1g4-52202511411459.jpg)
![Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Western blot analysis of extracts of various cell lines, using p23/PTGES3 Rabbit mAb (NBP3-16586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-10M9W8-Western-Blot-NBP3-16586-img0004.jpg "Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Western blot analysis of extracts of various cell lines, using p23/PTGES3 Rabbit mAb (NBP3-16586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of U-2 OS cells using p23/PTGES3 Rabbit mAb (NBP3-16586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-10M9W8-Immunocytochemistry-Immunofluorescence-NBP3-16586-img0003.jpg)
![Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Western blot analysis of extracts of various cell lines, using p23/PTGES3 Rabbit mAb (NBP3-16586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-10M9W8-Western-Blot-NBP3-16586-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of C6 cells using p23/PTGES3 Rabbit mAb (NBP3-16586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-10M9W8-Immunocytochemistry-Immunofluorescence-NBP3-16586-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of NIH-3T3 cells using p23/PTGES3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16586_rabbit-p23-ptges3-mab-10m9w8-52202511392233.jpg%3Ft=1698886044)
![Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Western-Blot-NBP2-61122-img0004.jpg "Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61122-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Tissue: Colon Cancer. Species: Human. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody (NBP2-61122) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunohistochemistry-Paraffin-NBP2-61122-img0003.jpg)
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32511 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32511 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32511 in HeLa cell lysate Lane 3: NBP3-32511 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-2682024158263.jpg "Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32511 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32511 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32511 in HeLa cell lysate Lane 3: NBP3-32511 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Western blot analysis of Importin beta/KPNB1 on different lysates with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32511) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Caco-2 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Mouse testis tissue lysate (40 ug/Lane) Lane 13: Rat spleen tissue lysate (40 ug/Lane) Lane 14: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32511) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-27820241628523.jpg)
![Immunohistochemistry: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling Importin beta/KPNB1 with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32511) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32511, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-29820241420963.jpg)
![Immunohistochemistry: Importin beta/KPNB1 Antibody (PSH01-60) [NBP3-32511] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32511) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32511) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32511_rabbit-importin-beta-kpnb1-mab-psh01-60-29820241435256.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Western-Blot-NBP2-99436-img0006.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in rat kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0005.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0002.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0003.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0004.jpg)
![Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Western-Blot-NBP2-50053-img0002.jpg "Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, NBP2-50053, dilution 1:500 in green and costained with chicken pAb to lamin A/C, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin AC antibody stains the nuclear lamina and membrane.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - HeLa cell cultures were stained with NBP2-50053 (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, green). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0003.jpg)
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-262202415355731.jpg "Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-26220241522415.jpg)
![Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate. All lanes: HSP90 beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 80-90 kDa](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202411485820.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-28220241312214.jpg)
![Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202414312789.jpg)