| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47470SS | |||
| Azide and BSA Free | NBP2-80757 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81946-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70572 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86030-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35458-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38220-25ul | |||
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0012.jpg "Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunocytochemistry-Immunofluorescence-NBP1-47470-img0006.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas and GSK-3 beta in a murine xenograft model. SW620 cells injected into flanks of athymic Nude mice & tumor volume was checked biweekly. Mice treated weekly with oxaliplatin (days 0, 7 and 14) & injected daily with Fap1 blocking SLV peptide, VLS control peptide, or treated with SLV or VLS peptide alone (n=12). Tumors were simultaneously harvested when control tumors were >2,000 mm3. SLV peptide increases Gsk3-phosphorylation with/without oxaliplatin. IF detection of total versus phospho- GSK-3 beta performed with DAPI staining of nuclei (areas without gland formation were selected). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25401), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-NBP1-47470-img0014.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0013.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-Paraffin-NBP1-47470-img0007.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0005.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0009.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases Fas & Gsk3 beta phosphorylation in CD133+ cells in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases Fas phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Fas or CD133 was performed with DAPI staining of nuclei. (B) SLV peptide increases Gsk3 beta phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Gsk3 beta or CD133 was performed with DAPI staining of nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-310202416171462.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas & Gsk3 beta in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases gland formation in xenograft tumors with or without oxaliplatin. Histology was analyzed by hematoxylin/ eosin staining. Fap1 expression was determined by immunofluorescence. Relative fluorescent intensity (RFI) of Fap1 staining is indicated below relevant panels. (B) SLV peptide increases Fas-phosphorylation in xenograft tumors with or without by oxaliplatin. Immunofluorescent detection of total versus phospho-Fas was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. (C) SLV peptide increases Gsk3 beta -phosphorylation with or without oxaliplatin. Immunofluorescent detection of total versus phospho- Gsk3 beta was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-3102024161604.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523259.jpg "Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.")
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523253.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523256.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523250.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-2342024155514.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-234202415232519.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines, using NB100-81946](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-103202523472617.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202594524.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145457.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145471.jpg)
![Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Western-Blot-NBP2-02705-img0013.jpg "Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.")
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0012.jpg)
![Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Flow-Cytometry-NBP2-02705-img0002.jpg)
![Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Western-Blot-NBP2-02705-img0003.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0004.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0005.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0006.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0007.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0008.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0009.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0010.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Immunohistochemistry-Paraffin-NBP2-02705-img0011.jpg)
![Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - Analysis of Jurkat cells, using anti-CYB5R3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CYB5R3-Antibody-2A10-Flow-Cytometry-NBP2-02705-img0001.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0015.jpg "Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta stained by GSK3 beta antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0013.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - H661 xenograft, using GSK3 beta antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0009.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody [NBP1-31353] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3].](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Rat brain. GSK3 beta antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0007.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0005.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0001.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0007.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0004.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0017.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-32695] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32695-img0001.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-32695] - A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-32695-img0013.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0015.jpg)
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP1-32695] - IP samples: HeLa whole cell extract A. 40 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 ug 14-3-3 epsilon antibody [N1C3] (NBP1-32695) 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (NBP1-32695) diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP1-32695-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-89827] - Staining 14-3-3 epsilon in methanol-fixed, rat primary motor neurons using anti-14-3-3 epsilon antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89827-img0010.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-89827] - Staining 14-3-3 epsilon in methanol-fixed, rat primary motor neurons using anti-14-3-3 epsilon antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.")
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-89827-img0009.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Analysis in human lymph node and pancreas tissues. Corresponding PLC-gamma 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Analysis in human lymph node and pancreas tissues. Corresponding PLC-gamma 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0005.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human duodenum shows strong cytoplasmic positivity in lymphoid cells and immunoreactivity in endocrine cells.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0009.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody detects GSK-3 beta protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK-3 beta stained by GSK-3 beta antibody (NBP3-13679) diluted at 1:500.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunocytochemistry-Immunofluorescence-NBP3-13679-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0003.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0001.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [NBP3-35458] - Immunofluorescence analysis of paraffin-embedded rat lung using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35458_rabbit-12-lipoxygenase-pab-1012202411392935.jpg "Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [NBP3-35458] - Immunofluorescence analysis of paraffin-embedded rat lung using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35458_rabbit-12-lipoxygenase-pab-1012202411353075.jpg)
![Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35458_rabbit-12-lipoxygenase-pab-1012202411421056.jpg)
![Western Blot: 12-Lipoxygenase Antibody [NBP3-35458] - Western blot analysis of lysates from A-431 cells, using 12-Lipoxygenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35458_rabbit-12-lipoxygenase-pab-1012202411433410.jpg)
![Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] - Immunohistochemistry analysis of paraffin-embedded Rat heart using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35458_rabbit-12-lipoxygenase-pab-1012202411334038.jpg)
![Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-288202412461651.jpg "Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32827) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-298202414333622.jpg)
![Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunocytochemistry analysis of Raji cells labeling PLC-gamma 2 with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-29202410334047.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Flow cytometric analysis of Raji cells labeling PLC-gamma 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32827, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-392024884627.jpg)
![Western Blot: PLC-beta 1 Antibody [NBP2-38220] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma](http://images.novusbio.com/fullsize/PLC-beta-1-Antibody-Western-Blot-NBP2-38220-img0002.jpg "Western Blot: PLC-beta 1 Antibody [NBP2-38220] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma")
