| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48308SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-125SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70169 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB400-140SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86177-25ul | |||
![Western Blot: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Knockout-Validated-NB200-157-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NB200-157-img0009.jpg)
![Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-NB200-157-img0010.jpg)
![Western Blot: Rabbit Polyclonal 4EBP1 Antibody [NB200-157] - Whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. 4EBP1 Antibody (NB200-157) was used for primary antibody: 1:2000, 4C, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb200-157_rabbit-polyclonal-4ebp1-antibody-western-blot-311202415623..png)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0018.jpg "Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0007.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunohistochemistry-NBP1-48308-img0009.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0019.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0010.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0012.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor (T) and the adjacent normal tissues (N) is shown. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0016.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2a. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in HeLa cells.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0008.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of staining with anti-PKM2 is shown. The boxed regions are enlarged in the images below. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25721733/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2 Antibody - BSA Free-Immunohistochemistry-NBP1-48308-img0020.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0014.jpg)
![Simple Western: PKM2 Antibody - BSA Free [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Simple-Western-NBP1-48308-img0013.jpg)
![Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0003.jpg "Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0005.jpg)
![Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody [NBP1-87102] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining Is shown in green.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87102-img0018.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Western-Blot-NBP2-01740-img0012.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-01740-img0011.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0008.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 Lipoxygenase 1.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0009.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0002.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0005.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0006.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0007.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0011.jpg "Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.")
![Immunocytochemistry/Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-140-img0013.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0005.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Analysis of Cyp-46 in HEK293 cells overexpressing CYP46A1 or transfected with an empty vector using NB400-140.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0010.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP1-86177] - Staining of human cell line A-431 shows localization to cytosol & rods & rings.](http://images.novusbio.com/fullsize/IMP-Dehydrogenase-2-IMPDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86177-img0008.jpg "Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP1-86177] - Staining of human cell line A-431 shows localization to cytosol & rods & rings.")
