| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70068 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89526-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22033-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87293-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Western-Blot-NBP2-75578-img0011.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0009.jpg)
![Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Flow-Cytometry-NBP2-75578-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0005.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0006.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0008.jpg)
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0008.jpg "Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunocytochemistry-Immunofluorescence-NBP2-02588-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0006.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0002.jpg)
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0004.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0005.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - Analysis of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0001.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar protein distribution across tissues to independent antibody NBP1-89526 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT2 Antibody [NBP1-89525] - Analysis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar pattern to independent antibody NBP1-85967 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP1-89525-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0019.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0020.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0013.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31378-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-31378-img0007.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0008.jpg)
![Immunoprecipitation: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 ug of AKR1C3 antibody [C2C3], C-term. Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunoprecipitation-NBP1-31378-img0012.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Aldo-keto Reductase 1C3/AKR1C3 antibody [C2C3], C-term (NBP1-31378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31378_rabbit-polyclonal-aldo-keto-reductase-1c3-akr1c3-antibody-western-blot-141120251844347.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89526-img0006.jpg "Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89526 (A) shows similar protein distribution across tissues to independent antibody NBP1-89525 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0013.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0015.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0014.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0016.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0023.jpg "Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0016.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0019.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-57202317215160.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: MCF-7. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462214.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: SH-SY5Y. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-2342024155511.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Hep-G2. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462212.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Mouse lung. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202415232515.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Rat kidney. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462210.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249432858.jpg "Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-21202494149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-212024941459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249422638.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Muscle Phosphofructokinase/PFKM/PFK-1 antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-western-blot-1411202518312123.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0012.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87293-img0013.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87293-img0010.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)
![Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP2-32198-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-NBP2-32198-img0002.jpg)