| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56011-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71749 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92668-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97309SS | |||
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-4r3l10-western-blot-28202392140..jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361219.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511402799.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361234.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511411465.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361267.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0004.jpg "Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded mouse spleen using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0002.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Rat lung, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded human appendix using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Confocal imaging of U-2 OS cells using 5-Lipoxygenase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16150_rabbit-5-lipoxygenase-mab-5c5z2-5220251242536.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Western-Blot-NBP2-88931-img0006.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Analysis in human cell line CACO-2.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Analysis in human kidney and skeletal muscle tissues using NBP2-88931 antibody. Corresponding AMACR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Immunohistochemistry-Paraffin-NBP2-88931-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Immunohistochemistry-Paraffin-NBP2-88931-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Staining of human liver cancer shows strong granular cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Immunohistochemistry-Paraffin-NBP2-88931-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Immunohistochemistry-Paraffin-NBP2-88931-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) [NBP2-88931] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9362-Immunohistochemistry-Paraffin-NBP2-88931-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56011-img0001.jpg "Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Analysis in human spleen and skeletal muscle tissues using NBP2-56011 antibody. Corresponding ALOX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0009.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0004.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human lung shows strong nuclear positivity in macrophages.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0006.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human small intestine shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0008.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-5F10-Western-Blot-NBP2-45491-img0003.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR.")
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-5F10-Immunohistochemistry-NBP2-45491-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-5F10-Western-Blot-NBP2-45491-img0004.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-5F10-Immunohistochemistry-NBP2-45491-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-2A10F3-Western-Blot-NBP1-28884-img0008.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Confocal immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-2A10F3-Immunocytochemistry-Immunofluorescence-NBP1-28884-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis of human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-2A10F3-Immunohistochemistry-Paraffin-NBP1-28884-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis of human brain cerebellum using AMACR mouse mAb.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-2A10F3-Immunohistochemistry-Paraffin-NBP1-28884-img0006.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-western-blot-2212025105133..png "Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of NIH/3T3 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-4220251425187.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of U2OS cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514264439.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of PC-12 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514275258.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Western-Blot-NBP2-73228-img0001.jpg "Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0005.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0002.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0004.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0006.jpg)
![Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.](http://images.novusbio.com/fullsize/PAD4-Antibody-Immunohistochemistry-NBP1-97309-img0001.jpg "Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.")