| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89324-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40765SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87871-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73394 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0007.jpg "Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color")
![Immunohistochemistry: CPT1B Antibody [NBP1-59576] - searcher: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE Application: IHC Species+tissue/cell type:Species+tissue/cell type: Human Capan1 cells Primary antibody dilution: 1:300 Secondary antibody: Anti-rabbit Alexa Fluor 488 Secondary antibody dilution:1:200](http://images.novusbio.com/fullsize/CPT1B-Antibody-Immunohistochemistry-NBP1-59576-img0004.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0003.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Western-Blot-NBP1-89324-img0010.jpg "Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody [NBP1-89324] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89324-img0009.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0014.jpg)
![Western Blot: PRKAG2 Antibody [NBP1-89324] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Western-Blot-NBP1-89324-img0007.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0011.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0012.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0013.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40765-img0003.jpg "Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40765-img0004.jpg)
![Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Simple-Western-NB110-40765-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [NBP2-15252] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15252-img0008.jpg "Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [NBP2-15252] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Human breast carcinoma. ACSL3 stained by ACSL3 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP2-15252-img0009.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - IHC analysis of human breast cancer tissue section, using ACSL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer for 15 min.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP2-15252-img0001.jpg)
![Simple Western: ACSL3 Antibody [NBP2-15252] - Mouse brain (hippocampus) tissue. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Simple-Western-NBP2-15252-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] Mouse stomach. ACSL3 stained by ACSL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15252_rabbit-polyclonal-acsl3-antibody-immunohistochemistry-paraffin-118202381849..jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Rat colon.ACSL3 stained by ACSL3 antibody diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15252_rabbit-polyclonal-acsl3-antibody-immunohistochemistry-paraffin-11820238229..jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15252_rabbit-polyclonal-acsl3-antibody-910202420115023.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15252_rabbit-polyclonal-acsl3-antibody-91020242014680.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0013.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0010.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0011.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16400-img0002.jpg "Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0011.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0001.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0007.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] diluted at 1:500](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0010.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251827016.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251823622.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518204710.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518422918.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0026.jpg "Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0023.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0013.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Phosphoribosyl Pyrophosphate Amidotransferase.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0025.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0014.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0015.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0018.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0021.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0022.jpg)
![Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FACL4-Antibody-3I8D10-Immunoprecipitation-NBP3-16012-img0002.jpg "Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.")
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-5220251718945.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184534.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517202918.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184563.jpg)
![Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172885.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213510.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213524.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172887.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")