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![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-V17-1-Immunohistochemistry-Paraffin-NBP2-44962-img0002.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).")
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp3-09106_mouse-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-w8b2b10-flow-cytometry-1212202415932..jpg "Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).")
![Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Anti-Hu TNAP purified antibody (concentration in sample 0.3 ug/ml, GAM APC, red-filled histogram) binds specifically to TNAP on subpopulation of HeLa cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).](http://images.novusbio.com/fullsize/antibody/nbp3-09106_mouse-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-w8b2b10-flow-cytometry-12122024151035..jpg)
![Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-9R8N3-Western-Blot-NBP3-16311-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Samples were spiked with high concentrations of Mouse S1P1/EDG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82426_mouse-s1p1-edg-1-elisa-kit-colorimetric-132202416121344.jpg "ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Samples were spiked with high concentrations of Mouse S1P1/EDG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82426_mouse-s1p1-edg-1-elisa-kit-colorimetric-4320249133224.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58024-img0001.jpg "Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-4F2-Western-Blot-NBP3-15216-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.")