| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77459SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59779 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61620 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
![Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Western-Blot-NB100-2462-img0004.jpg "Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Immunohistochemistry-Paraffin-NB100-2462-img0006.jpg)
![Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Western-Blot-NB100-2462-img0003.jpg)
![Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Phosphodiesterase-4D-Antibody-Immunohistochemistry-Paraffin-NB100-2462-img0005.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0002.jpg "Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NB100-2422] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2422-img0003.jpg)
![Flow Cytometry: PCK2 Antibody [NB100-2422] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Flow-Cytometry-NB100-2422-img0004.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of A431 (A), HEK293 (B) with antibody at 2 ug/mL and HepG2 (C) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0001.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-54318-img0008.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54318-img0006.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0005.jpg "Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP1-31241] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31241-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP1-31241] - OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-31241-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0003.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-32721] - Paraffin-embedded mouse liver. liver FABP antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-32721-img0004.jpg "Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-32721] - Paraffin-embedded mouse liver. liver FABP antibody [N1C3] diluted at 1:500.")
![Immunohistochemistry-Frozen: FABP1/L-FABP Antibody [NBP1-32721] - Frozen section of rat kidney. L-FABP antibody [N1C3] diluted at 1:200.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Frozen-NBP1-32721-img0003.jpg)
![Western Blot: FABP1/L-FABP Antibody [NBP1-32721] -Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with L-FABP antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32721_rabbit-polyclonal-fabp1-l-fabp-antibody-western-blot-127202385349..jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-32721] -Mouse liver. L-FABP stained by L-FABP antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-32721_rabbit-polyclonal-fabp1-l-fabp-antibody-immunohistochemistry-paraffin-127202385525..jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-32721] -Rat liver. L-FABP stained by L-FABP antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-32721_rabbit-polyclonal-fabp1-l-fabp-antibody-immunohistochemistry-paraffin-127202385634..jpg)
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77459-img0008.jpg "Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - Analysis of heme oxygenase mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-NBP1-77459-img0007.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0006.jpg "Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-19729] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-19729-img0004.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0001.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0002.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/nbp3-43847_mouse-cox4-mab-cl3515-azide-and-bsa-free-282202512104221.jpg "Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518415223.jpg "<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518335915.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842516.jpg)
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251839419.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518392014.jpg)
![P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-immunocytochemistry-immunofluorescence-1411202518333113.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842412.jpg)