| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73344 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73226 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52508-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48873-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90125-img0015.jpg "Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.")
![Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/PFKP-Antibody-OTI1D6-Western-Blot-NBP2-01539-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-01539-img0012.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0009.jpg)
![Flow Cytometry: PFKP Antibody (1D6) [NBP2-01539] - Analysis of Jurkat cells, using anti-PFKP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Flow-Cytometry-NBP2-01539-img0001.jpg)
![Western Blot: PFKP Antibody (1D6) [NBP2-01539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Western-Blot-NBP2-01539-img0002.jpg)
![Western Blot: PFKP Antibody (1D6) [NBP2-01539] Analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Western-Blot-NBP2-01539-img0011.jpg)
![Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Analysis of extracts (10ug) from 9 Human tissues by using anti-PFKP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/PFKP-Antibody-OTI1D6-Western-Blot-NBP2-01539-img0014.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0003.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0004.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0005.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0006.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0007.jpg)
![Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFKP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PFKP-Antibody-1D6-Immunohistochemistry-Paraffin-NBP2-01539-img0008.jpg)
![PFKP Antibody (OTI1D6)[NBP2-01539]Simple Western� analysis of endogenous protein DGKA from Jurkat lysates (0.2 mg/mL) using DGKA Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-01539_mouse-pfkp-mab-oti1d6-simple-western-131120251236358.jpg%253fstore=america&image-type=image)
![Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Western-Blot-NBP2-02082-img0025.jpg "Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-02082-img0016.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0027.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0014.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0017.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0019.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0024.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0015.jpg)
![Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Western-Blot-NBP2-52508-img0007.jpg "Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)")
![Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunohistochemistry-NBP2-52508-img0006.jpg)
![Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Flow-Cytometry-NBP2-52508-img0001.jpg)
![Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against HEK293 (1) and MAP2K3 (AA: 1-138)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Western-Blot-NBP2-52508-img0008.jpg)
![Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunohistochemistry-Paraffin-NBP2-52508-img0005.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of GC-7901 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0002.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0003.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of HepG2 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0004.jpg)
![ELISA: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-ELISA-NBP2-52508-img0009.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0004.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70169-img0005.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0011.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0002.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0006.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0007.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0008.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0009.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0010.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0012.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Detection of LOX5 in human peripheral leukocytes using NB110-58749 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0002.jpg)
![Simple Western: 5-Lipoxygenase Antibody [NB110-58749] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Simple-Western-NB110-58749-img0003.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0014.jpg "Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00004830-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunohistochemistry-Paraffin-H00004830-M02-img0009.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0012.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0013.jpg)
![Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunoprecipitation-H00004830-M02-img0010.jpg)
![ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-ELISA-H00004830-M02-img0011.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-48873] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-48873-img0003.jpg "Western Blot: Thioredoxin Antibody [NBP2-48873] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-48873] - Staining of human cell line U-251 MG shows localization to nucleus and cytosol. Antibody staining is shown in greeen.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48873-img0009.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human kidney.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0012.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0006.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining in human esophagus and skeletal muscle tissues using anti-TXN antibody. Corresponding TXN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human colon, esophagus, kidney and skeletal muscle using Anti-TXN antibody NBP2-48873 (A) shows similar protein distribution across tissues to independent antibody NBP2-49191 (B).](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0010.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human colon.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0011.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)