| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49533SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48308SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58748SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71189 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74505 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22214-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33948-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32026-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31974-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
![Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0015.jpg "Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49533-img0011.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-NBP1-49533-img0005.jpg)
![Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533 (NBP1-49533AF647) on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody---BSA-Free-Flow-Cytometry-NBP1-49533-img0016.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0010.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0014.jpg)
![Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0008.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0012.jpg)
![Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Intracellular-NBP1-49533-img0013.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-221020258369.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-2210202583725.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0018.jpg "Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0007.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunohistochemistry-NBP1-48308-img0009.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0019.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0010.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0012.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor (T) and the adjacent normal tissues (N) is shown. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0016.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2a. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in HeLa cells.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0008.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of staining with anti-PKM2 is shown. The boxed regions are enlarged in the images below. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25721733/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM2 Antibody - BSA Free-Immunohistochemistry-NBP1-48308-img0020.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0014.jpg)
![Simple Western: PKM2 Antibody - BSA Free [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Simple-Western-NBP1-48308-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58748-img0008.jpg "Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Detection of LOX5 in human peripheral leukocytes using NB110-58748 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58748-img0003.jpg)
![Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Simple-Western-NB110-58748-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts from RASFs transfected with empty vector or 5-Lipoxygenase shRNA were used for Western blotting. Immunoblotting showed that cells transfected with 5-Lipoxygenase shRNA of clone No.3 and No.7 markedly decreased 5-Lipoxygenase expression. Human synovial fibroblasts transfected with empty vector or 5-Lipoxygenase shRNA were treated with TNF-alpha for 6 hr. IL-6 and MCP-1 protein release in the conditioned medium was evaluated by ELISA. Note that knockdown of 5-Lipoxygenase decreased IL-6. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC4168259/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Knockdown-Validated-NB110-58748-img0007.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0022.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.")
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded prostate tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0020.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Leukotriene A4 hydrolase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Leukotriene A4 hydrolase.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0021.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0013.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0014.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded endometrium tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0018.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0017.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded pancreas tissue using anti-Leukotriene A4 hydrolasemouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0019.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunoprecipitation-NBP1-47829-img0023.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Western-Blot-NBP2-02710-img0008.jpg "Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunocytochemistry-Immunofluorescence-NBP2-02710-img0007.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-02710-img0006.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Thromboxane synthase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0003.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Thromboxane synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Thromboxane synthase.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Western-Blot-NBP2-02710-img0004.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human Kidney tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-02710-img0005.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Analysis of Hela cells, using anti-Thromboxane synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0001.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Analysis of Jurkat cells, using anti-Thromboxane synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0002.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0008.jpg "Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0003.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0009.jpg)
![Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Flow-Cytometry-NBP2-22214-img0001.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0006.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0002.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0004.jpg)
![ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-ELISA-NBP2-22214-img0005.jpg)
![mTORC2 in adipose tissue is required for cold‐induced glucose uptake and glycolysis2‐deoxyglucose‐6‐phosphate (2DG6P) accumulation in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 6/group).Extracellular acidification rate (ECAR) of BAT explants from AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 7/group).Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of isolated plasma membranes from BAT of AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of mitochondrial and cytosolic fractions from BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Cytosolic hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Mitochondrial hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Data information: Data represent mean +/- SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (#P < 0.05; ##P < 0.01; ###P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26772600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22214_mouse-monoclonal-glut4-antibody-3g10a3-western-blot-132202615255419.jpg)
![Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Detection of LOX5 in human peripheral leukocytes using NB110-58749 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0002.jpg)
![Simple Western: 5-Lipoxygenase Antibody [NB110-58749] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Simple-Western-NB110-58749-img0003.jpg)
![Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0012.jpg "Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82487-img0009.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP1-82487-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0011.jpg)
![Western Blot: Thromboxane synthase Antibody [NBP2-33948] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Western-Blot-NBP2-33948-img0005.jpg "Western Blot: Thromboxane synthase Antibody [NBP2-33948] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human liver.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0010.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0001.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0006.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining in human spleen and skin tissues using anti-TBXAS1 antibody. Corresponding TBXAS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human colon, liver, skin and spleen using Anti-TBXAS1 antibody NBP2-33948 (A) shows similar protein distribution across tissues to independent antibody NBP2-33947 (B).](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0009.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody [NBP2-33948] - Staining of human colon.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-33948-img0011.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-32026-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-32026] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32026-img0007.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0012.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-32026] - Staining of human small intestine shows moderate cytoplasmic and luminal membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-32026-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar protein distribution across tissues to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0013.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-31974-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-31974] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31974-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0018.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-31974-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining in human small intestine and cerebral cortex tissues using anti-PLCB3 antibody. Corresponding PLCB3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar protein distribution across tissues to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0014.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0016.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0017.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)