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![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249413389.jpg "Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249415936.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-212024941421.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249374988.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249432829.jpg)
![Western Blot: EOMES Antibody [NBP3-13356] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EOMES antibody (NBP3-13356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EOMES-Antibody-Western-Blot-NBP3-13356-img0001.jpg "Western Blot: EOMES Antibody [NBP3-13356] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EOMES antibody (NBP3-13356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: EOMES Antibody [NBP3-13356] - EOMES antibody detects EOMES protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EOMES stained by EOMES antibody (NBP3-13356) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/EOMES-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13356-img0004.jpg)
![Immunohistochemistry-Paraffin: EOMES Antibody [NBP3-13356] - EOMES antibody detects TBR2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. TBR2 stained by EOMES antibody (NBP3-13356) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EOMES-Antibody-Immunohistochemistry-Paraffin-NBP3-13356-img0003.jpg)
![Immunohistochemistry-Paraffin: EOMES Antibody [NBP3-13356] - EOMES antibody detects TBR2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. TBR2 stained by EOMES antibody (NBP3-13356) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EOMES-Antibody-Immunohistochemistry-Paraffin-NBP3-13356-img0002.jpg)
![Western Blot: IL11RA Antibody [NBP1-62351] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/IL11RA-Antibody-Western-Blot-NBP1-62351-img0002.jpg "Western Blot: IL11RA Antibody [NBP1-62351] - Human Muscle lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: IL-11R alpha Antibody [NBP1-62351] - The effect of anti-human IL11Ralpha antibody combination treatment with doxorubicin on AN3CA xenograft tumour morphology in vivo. Representative photomicrographs of hematoxylin and eosin stained AN3CA subcutaneous tumour sections are shown in the top panel, arrows denote areas of tissue necrosis. AN3CA subcutaneous tumour sections were immunostained for pSTAT3, cleaved-caspase-3 (apoptosis marker) and Ki-67 (proliferation marker). Bars represent 100 um (H&E), 50 um (pSTAT3) and 200 um (caspase-3, Ki-67). Insets are negative controls. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/15187) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-11R-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-62351-img0003.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49955-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49955] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49955-img0003.jpg)
![Western Blot: TSLP Antibody [NBP1-76754] - A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NBP1-76754-img0002.jpg "Western Blot: TSLP Antibody [NBP1-76754] - A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Immunohistochemistry-Paraffin: TSLP Antibody [NBP1-76754] - Mouse brain tissue with TSLP antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/TSLP-Antibody-Immunohistochemistry-Paraffin-NBP1-76754-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NBP1-76754] - Figure 2 Immunofluorescence Validation of TSLP in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76754_rabbit-polyclonal-tslp-antibody-2711202419261873.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-2711202419381793.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241933343.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM , (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241928862.gif)
![Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Western-Blot-NBP3-14749-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0002.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0001.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]- Rabbit anti-CD127/IL-7R alpha recombinantmonoclonal antibody [BLR177J] Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14749_rabbit-il-7r-alpha-cd127-mab-immunohistochemistry-paraffin-1012024122450..jpg)
![Western Blot: CD19 Antibody (BLR137H) [NBP3-14717] - Whole cell lysate (10 ug) from Ramos, HeLa, HEK293T, Raji, Hep-G2, MCF-7, Jurkat, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Western-Blot-NBP3-14717-img0001.jpg "Western Blot: CD19 Antibody (BLR137H) [NBP3-14717] - Whole cell lysate (10 ug) from Ramos, HeLa, HEK293T, Raji, Hep-G2, MCF-7, Jurkat, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (BLR137H) [NBP3-14717] - Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H. ]Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Immunocytochemistry-Immunofluorescence-NBP3-14717-img0003.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (BLR137H) [NBP3-14717] - Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Immunohistochemistry-Paraffin-NBP3-14717-img0002.jpg)
![Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Western-Blot-NBP3-15441-img0002.jpg "Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunofluorescence analysis of NIH-3T3 cells using VPS34 Rabbit mAb (NBP3-15441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunocytochemistry-Immunofluorescence-NBP3-15441-img0001.jpg)
![Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug VPS34 antibody (NBP3-15441). Western blot was performed from the immunoprecipitate using VPS34 antibody (NBP3-15441) at a dilition of 1:500.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunoprecipitation-NBP3-15441-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of C2C12 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15441_rabbit-vps34-mab-9u5o3-52202517235772.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of PC-3 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15441_rabbit-vps34-mab-9u5o3-52202517273563.jpg)
![Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Western-Blot-NBP3-15612-img0004.jpg "Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0001.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: BTK Antibody (2J9O8) [BTK] - Immunofluorescence analysis of paraffin-embedded mouse spleen using BTK Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15612_rabbit-btk-mab-2j9o8-52202517311037.jpg)
![Western Blot: IKK gamma Antibody [NB600-216] - WB detection of IKK-gamma in Interleukin 1 stimulated HEK 293 cells at a dilution of 1:500.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NB600-216-img0002.jpg "Western Blot: IKK gamma Antibody [NB600-216] - WB detection of IKK-gamma in Interleukin 1 stimulated HEK 293 cells at a dilution of 1:500.")