| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25242 | |||
| BSA Free | NBP2-25236SS | |||
| Azide and BSA Free | NBP2-25242SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47910-0.1mg | |||
| IHC-Prediluted | NBP2-48332 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71021 | |||
![Western Blot: CD69 Antibody (15B5G2) [NBP2-25236] - Analysis using Azide Free version of NBP2-25236. CD69 in A) a partial recombinant protein and B) ThP1 lysate using CD69 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CD69-Antibody-15B5G2-Western-Blot-NBP2-25236-img0004.jpg "Western Blot: CD69 Antibody (15B5G2) [NBP2-25236] - Analysis using Azide Free version of NBP2-25236. CD69 in A) a partial recombinant protein and B) ThP1 lysate using CD69 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: CD69 Antibody (15B5G2) [NBP2-25236] - Human tonsil stained with CD69 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/CD69-Antibody-15B5G2-Immunohistochemistry-Paraffin-NBP2-25236-img0002.jpg)
![Western Blot: CD69 Antibody (15B5G2) [NBP2-25236] - Analysis of CD69 in A) a partial recombinant protein and B) ThP1 lysate using CD69 antibody at 0.5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CD69-Antibody-15B5G2-Western-Blot-NBP2-25236-img0003.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0005.jpg "Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.")
![Immunohistochemistry: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Immunohistochemistry-NB400-152-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cathepsin E Antibody [NB400-152] - The expression and methylation status of Cathepsin E gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) and C57BL/6 (B6) mice. mRNA expression of Cathepsin E in CD4+, CD8+, B cells and macrophages in B6 and MRL mice. ***p<0.001. Western blot analyses of Cathepsin E in CD4+, CD8+, B cells and macrophages in B6, MRL and mice. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-38809-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0007.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E on mouse stomach lysate.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0001.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - The expression & methylation status of Ctse gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) & C57BL/6 (B6) mice. (A) mRNA expression of Ctse in CD4+, CD8+, B cells & macrophages in B6 & MRL mice. ***p < 0.001. (B) Western blot analyses of CTSE in CD4+, CD8+, B cells & macrophages in B6, MRL & mice. (C) Methylated region expanding 583 bp in Ctse intron 1 in B6 mice demonstrated by UCSC Genome Browser. In MRL mice, 583 bp region is hypomethylated. Duplicate reads are indicated by arrows. (D) Nucleotide sequence within 583 bp flanking region of Ctse intron 1 in B6 mice. Among 13 CpGs (red & underlined), 11th CpG (boxed) includes mCGCG binding motif for Kaiso. After genomic DNAs were bisulphite converted, amplified by PCR using Primer F1 & R1, subcloned, & isolated clones sequenced. 98.6% of CpGs (71 out of 72 colonies) in B6 mice & 76.9% CpGs (50 of 65) in MRL mice were methylated. (E) The structure of Kaiso, SMRT (Silencing Mediator of Retinoic acid & Thyroid hormone receptors)/N-CoR (Nuclear hormone receptor Co-Repressor), & HDAC3 (Histone deacetylase 3) complex. BTB (for BR-C, ttk & bab), POZ (for Pox virus & Zinc finger) & C2H2 zinc-finger domains are shown. (F) DNA sequencing of bisulphite converted DNAs in Ctse intron 1 in B6 & MRL mice. Percentage of methylation of 11th CpG is shown (n = 5). ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30816218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-152_rabbit-polyclonal-cathepsin-e-antibody-310202416161819.jpg)
![Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-NBP2-44568-img0005.jpg "Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Placenta stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunofluorescence-NBP2-44568-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - Immunofluorescence staining of retained human SVF adjacent to the site of cell-seeded scaffold transplantation. (A) Human SVF are visualized using human specific nuclear matrix antigen (NuMA). Immunofluorescence imaging of human-specific antibodies targeting CD34 (B) & CD31 (C). Scale bar: 50 μm (A), 100 μm (B,C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44568_mouse-monoclonal-cd34-antibody-hpca1-763-31020241535640.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NCAM-1/CD56 Antibody [NBP2-38452] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Western-Blot-NBP2-38452-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38452-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0015.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0012.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0014.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0008.jpg "Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)")
![Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400] - IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunofluorescence-NBP2-45400-img0001.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0006.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0007.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0002.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0003.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0004.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0005.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0002.jpg "Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]")
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0001.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Sections of nasal biopsies were incubated with antibodies against TLR9 (C) visualized by 3, 3-diaminobenzidine (brown). In control slides (F), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0098239) licensed under a CC-BY license. TLR7 Antibody [NB100-56682]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0003.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz. TLR7 Antibody [NBP2-24767]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0004.jpg)
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary. TLR7 Antibody [NB100-56588]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0005.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: NLRP9 Antibody [NBP2-24661] - Analysis of NBP2-24661 in Jurkat lysate in the 1) absence and 2) presence of immunizing peptide probed with NBP2-24661 antibody 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NLRP9-Antibody-Western-Blot-NBP2-24661-img0001.jpg "Western Blot: NLRP9 Antibody [NBP2-24661] - Analysis of NBP2-24661 in Jurkat lysate in the 1) absence and 2) presence of immunizing peptide probed with NBP2-24661 antibody 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: NLRP9 Antibody [NBP2-24661] - Formalin-fixed, paraffin-embedded human testis stained with NALP9 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/NLRP9-Antibody-Immunohistochemistry-Paraffin-NBP2-24661-img0002.jpg)