| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31372SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56564SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25241SS | |||
| Azide and BSA Free | NBP2-80965 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34413-0.1mg | |||
![Western Blot: NALP6 Antibody [NBP2-31372] - WB detection of NALP6 protein (NACHT, LRR and PYD domains-containing protein 6) in (A) Jurkat cells lysate and (B) partial recombinant protein using NALP6 antibody at a concentration of 1 ug/ml for lysate and 0.1 ug/ml for the recombinant protein. In Jurkat cells lysate, this antibody detected a major band at ~98.8 kDa which the expected position for NALP6.](http://images.novusbio.com/fullsize/NALP6-Antibody-Western-Blot-NBP2-31372-img0001.jpg "Western Blot: NALP6 Antibody [NBP2-31372] - WB detection of NALP6 protein (NACHT, LRR and PYD domains-containing protein 6) in (A) Jurkat cells lysate and (B) partial recombinant protein using NALP6 antibody at a concentration of 1 ug/ml for lysate and 0.1 ug/ml for the recombinant protein. In Jurkat cells lysate, this antibody detected a major band at ~98.8 kDa which the expected position for NALP6.")
![Immunohistochemistry-Paraffin: NALP6 Antibody [NBP2-31372] - IHC-P analysis of NALP6 protein in a section of human breast normal tissue using NALP6 antibody at a concentration of 5 ug/ml. The breast ductal /acinar epithelium showed a strong NALP6 positivity in the cytoplasm and nuclei of the cells.](http://images.novusbio.com/fullsize/NALP6-Antibody-Immunohistochemistry-Paraffin-NBP2-31372-img0002.jpg)
![Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Knockout-Validated-NB100-56564-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Western-Blot-NB100-56564-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Immunohistochemistry-Paraffin-NB100-56564-img0004.jpg)
![Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Western-Blot-NBP2-25241-img0001.jpg "Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.")
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Immunocytochemistry-Immunofluorescence-NBP2-25241-img0003.jpg)
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-BL-178-12C7-Western-Blot-NBP3-14668-img0013.jpg "Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody [BL-178-12C7](A700-012). Secondary: HRP-conjugated goat anti-rabbitIgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0006.jpg)
![Immunohistochemistry: CD45 Antibody [NBP3-14668] - Sample: FFPE section of human coloncarcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™.Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP3-14668] - Sample: Jurkat cells were fixed in 4% formaldehyde andpermeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45recombinant monoclonal antibody (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP3-14668-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0004.jpg)
![Immunoprecipitation: CD45 Antibody [NBP3-14668] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from Jurkat cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-CD45recombinant monoclonal antibody used for IP at 20 ul/mg lysate. CD45 wasalso immunoprecipitated by a previous lot of this antibodyand rabbit anti-CD45 antibody. For blotting immunoprecipitated CD45,was used at 1:1000. Chemiluminescence withan exposure time of 250 milliseconds.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunoprecipitation-NBP3-14668-img0002.jpg)
![Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Western-Blot-NBP2-34365-img0004.jpg "Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Immunohistochemistry-Paraffin-NBP2-34365-img0001.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB110-93480-img0001.jpg "Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.")
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Showing specific labeling of GFAP (red) and vimentin (green) cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000, green), colabeled with anti-MeCP2 (1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000,green), colabeled with anti-calbindin (1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0005.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0009.jpg "Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0008.jpg)
![Flow Cytometry: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Flow-Cytometry-NBP2-67650-img0001.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0005.jpg)
![Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Western-Blot-NBP2-66961-img0011.jpg "Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate")
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0010.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0014.jpg)
![Flow Cytometry: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Flow-Cytometry-NBP2-66961-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0008.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0009.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunofluorescence staining of paraffin- embedded human colon using anti-MUC2 rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/100 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0012.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0013.jpg)