| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33157 | |||
| IHC-Prediluted | NBP2-44428 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34401-0.1mg | |||
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0008.jpg "Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP1-78427-img0003.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0007.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0009.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. High power.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-Immunocytochemistry-Immunofluorescence-NBP2-39099-img0013.jpg "Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. High power.")
![Immunohistochemistry-Paraffin: CD163 Antibody (ED2) - BSA Free [NBP2-39099] - Alcohol-fixed paraffin embedded spleen tissue from healthy rat spleen. Primary antibody dilution: 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-BSA-Free-Immunohistochemistry-Paraffin-NBP2-39099-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. Low power.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-Immunocytochemistry-Immunofluorescence-NBP2-39099-img0012.jpg)
![Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Staining of acetone fixed, cryostat sectioned rat spleen.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-Immunohistochemistry-Frozen-NBP2-39099-img0014.jpg)
![Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Immunoperoxidase staining of rat lymph node cryosection (NBP2-39099) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-Immunohistochemistry-Frozen-NBP2-39099-img0015.jpg)
![Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Immunoperoxidase staining of rat lymph node cryosection (NBP2-39099) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power.](http://images.novusbio.com/fullsize/CD163-Antibody-ED2-Immunohistochemistry-Frozen-NBP2-39099-img0016.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0003.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.")
![Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Western-Blot-NBP2-25221-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (CCP58).](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0001.jpg)
![Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Western-Blot-NBP2-67639-img0007.jpg "Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.")
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of HUVEC cells labeling NLRP3/NAPL3 with Rabbit anti-NLRP3 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0011.jpg)
![Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Flow-Cytometry-NBP2-67639-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0006.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0004.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0010.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0005.jpg "Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.")
![Western Blot: HLA G Antibody (MEM-G/1) [NB500-302] - Using the Biotin direct conjugate analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Western-Blot-NB500-302-img0003.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1B - first-trimester placenta (paraffin-embedded sections)](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0001.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0002.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) - BSA Free [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. (a) HLA-G immunostaining (brown color top panel) indicates the location of extravillous trophoblasts. PhosphoH3(Ser10) immunostaining (brown color lower 3 panels) identifies proliferating cells undergoing mitosis. In extravillous trophoblasts of explants treated with ELA proliferation appears to decrease. In control explants almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA. Treatment with ELA in combination with ML221 does not return villous cytotrophoblast proliferation to the level as seen in control explants. (b) Quantifying the percentage of proliferating extravillous trophoblasts observed by immunohistochemistry shows a significant decrease upon treatment with ELA which cannot be rescued by the addition of ML221. P = 0.08 between controls & explants treated with ELA & ML221. n = 12 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with one-way ANOVA followed by Bonferroni multiple comparisons test. *Indicates p < 0.05. (c) Treatment of explants with an ELA neutralizing antibody shows a significant increase of extravillous trophoblast proliferation upon quantifying immunohistochemistry stainings. n = 4 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with a Student’s t-test. *Indicates p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31836787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-302_mouse-monoclonal-hla-g-antibody-mem-g-1-31020241617149.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45 antibody (2B11 + PD7/26) [NBP2-34287].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Immunohistochemistry-Paraffin-NBP2-34287-img0003.jpg "Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45 antibody (2B11 + PD7/26) [NBP2-34287].")
![Western Blot Analysis of human Spleen tissue lysates using CD45 antibody (2B11 + PD7/26) [NBP2-34287]. Observed molecular weight ~200 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Western-Blot-NBP2-34287-img0007.jpg)
![Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody(2B11 + PD7/26) [NBP2-34287].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Immunohistochemistry-Paraffin-NBP2-34287-img0001.jpg)
![Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 antibody (2B11 + PD7/26) [NBP2-34287] followed by Goat anti-Mouse-IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Flow-Cytometry-NBP2-34287-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (2B11 + PD7/26) [NBP2-34287] - Staining of human tonsil with Alexa Fluor 488 conjugate of CD45 Antibody (2B11 + PD7/26), pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34287_mouse-monoclonal-cd45-antibody-2b11-pd7-26-17720241003243.jpg)
![Western Blot: LYVE-1 Antibody Pack [NB100-939] - Total protein from human stomach, lymph node and placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LYVE1 (NB100-725) in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Western-Blot-NB100-939-img0017.jpg "Western Blot: LYVE-1 Antibody Pack [NB100-939] - Total protein from human stomach, lymph node and placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LYVE1 (NB100-725) in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human lung blood vessels using NB100-725. Note presence of RBCs within vessel lumen.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0004.jpg)
![Western Blot: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in mouse spleen lysate using NB 100-726. ECL detection overnight.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Western-Blot-NB100-939-img0018.jpg)
![Flow Cytometry: LYVE-1 Antibody Pack [NB100-939] - Analysis using the DyLight 488 conjugate of NB100-725. Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia Redondo.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Flow-Cytometry-NB100-939-img0019.jpg)
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human lung blood vessels using NB100-725.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0014.jpg)
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human bladder vasculature using NB100-725.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0002.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Western-Blot-NBP2-34353-img0003.jpg "Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM248) [NBP2-34353] - Formalin-fixed, paraffin-embedded human schwannoma stained with GFAP monoclonal antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Immunohistochemistry-Paraffin-NBP2-34353-img0001.jpg)
![Flow Cytometry: GFAP Antibody (SPM248) [NBP2-34353] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (SPM248) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Flow-Cytometry-NBP2-34353-img0002.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)