| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47774-0.1mg | |||
| IHC-Prediluted | NBP2-48224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64849SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50286-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31143SS | |||
| Azide and BSA Free | NBP2-80683 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31365SS | |||
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-1063-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0011.jpg)
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-1063-img0008.jpg)
![Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Flow-Cytometry-NB100-1063-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunocytochemistry-Immunofluorescence-NBP2-44502-img0006.jpg "Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (2F3.2) [NBP2-44502] - Human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunohistochemistry-Paraffin-NBP2-44502-img0001.jpg)
![Flow Cytometry: ZAP70 Antibody (2F3.2) [NBP2-44502] - Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Flow-Cytometry-NBP2-44502-img0005.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Simple Western lane view shows a specific band for ZAP70 in 0.2 mg/ml of Jurkat (left) and H. Tonsil (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0002.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Electropherogram image of the corresponding Simple Western lane. ZAP70 antibody was used at 10 ug/ml dilution of Jurkat and H. Tonsil lysates(s) respectively.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0003.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Lane view shows a specific band for ZAP70 in Jurkat cell lysate using 50 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 70 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/nbp2-44502_mouse-monoclonal-zap70-antibody-2f3-2-17720241003235.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lano Antibody [NBP1-88016] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/Lano-Antibody-Immunohistochemistry-Paraffin-NBP1-88016-img0013.jpg)
![Immunohistochemistry-Paraffin: Lano Antibody [NBP1-88016] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Lano-Antibody-Immunohistochemistry-Paraffin-NBP1-88016-img0012.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88016_rabbit-polyclonal-lano-antibody-65202516254511.jpg)
![ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.](http://images.novusbio.com/fullsize/nb100-64849_mouse-monoclonal-ctla-4-antibody-bni3-2312025235988.jpg "ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0006.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0005.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-87000_rabbit-polyclonal-zap70-antibody-304202513131169.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.")
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-40799-img0005.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0006.jpg)
![Western Blot: CTLA-4 Antibody (L4P2F5*F10) [NBP2-50286] - Analysis of CTLA-4 in Jurkat cell lysates. Two products are detected, slightly larger than anticipated-possibly due to glycosylation.](http://images.novusbio.com/fullsize/CTLA-4-Antibody-L4P2F5-F10-Western-Blot-NBP2-50286-img0001.jpg "Western Blot: CTLA-4 Antibody (L4P2F5*F10) [NBP2-50286] - Analysis of CTLA-4 in Jurkat cell lysates. Two products are detected, slightly larger than anticipated-possibly due to glycosylation.")
![Immunohistochemistry-Paraffin: CTLA-4 Antibody (L4P2F5*F10) [NBP2-50286] - Human lymph node cross section. Cytoplasmic staining is shown as expected (CTLA4 is expressed in T-cells). B. Positive cytoplasmic staining was also detected in human breast cancer sections.](http://images.novusbio.com/fullsize/CTLA-4-Antibody-L4P2F5-F10-Immunohistochemistry-NBP2-50286-img0002.jpg)
![ELISA: CTLA-4 Antibody (L4P2F5*F10) [NBP2-50286] - Detection of soluble CTLA4 in mouse serum by indirect ELISA.](http://images.novusbio.com/fullsize/CTLA-4-Antibody-L4P2F5-F10-ELISA-NBP2-50286-img0003.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal CTLA-4 Antibody (L4P2F5*F10) [NBP2-50286] - CTLA-4 was stained in human tonsil FFPE tissue. HIER antigen retrieval at pH 9 for 20min. Antibody was used in concentrations between 1:50-100. AF750 conjugated version of antibody was used (Catalog # NBP2-50286AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-50286_mouse-monoclonal-ctla-4-antibody-l4p2f5-f10-immunohistochemistry-paraffin-112202315557..jpg)
![Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/CTLA4-Antibody-37B3D8-2F2-Western-Blot-NBP2-31143-img0001.jpg "Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.")
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0004.jpg "Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Immunocytochemistry-Immunofluorescence-NBP3-13623-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0001.jpg)
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0003.jpg)
![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.](http://images.novusbio.com/fullsize/CD137-Antibody-Western-Blot-NBP2-31365-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.")