| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89726SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71044 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71259 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87061-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49383-25ul | |||
![Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Simple-Western-NB200-179-img0005.jpg "Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) and patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: x250 (a, b), x100 (c, d). Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0015.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its expression. Double-immunofluorescence images show staining for 15-PGDH/HPGD-positive (red) cells and cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, and endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: x400. Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0016.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NB200-179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis . Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB200-179-img0003.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0009.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - A representative western blot and densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, and the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) and 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta-actin to ensure equal loading. Statistical analysis was conducted using an unpaired t-test; *P=0.05, **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD Antibody-Knockdown Validated-NB200-179-img0018.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291984.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415384117.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241539722.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291976.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241621232.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of LTC4 on 15-PGDH expression in the colon cancer cell line Caco-2(A) Quantification by qPCR of the mRNA expression of 15-PGDH following treatment with 40 nM LTC4 for 48 h in the presence or absence of AP100984 (a CysLT2 receptor antagonist, 1 μM) & JNK inhibitor I (10 μM). (B) Western blot showing 15-PGDH expression (antibody dilution, 1:5000) & densitometric analysis of LTC4-induced 15-PGDH up-regulation before or after the cells were stimulated with 40 nM LTC4 in the presence or absence of AP100984 & JNK inhibitor I for 48 h. (C) Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. The data are presented as the percent of untreated control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01, ***P<0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205174.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of 15-PGDH down-regulation on the cell differentiation markers SI & Mucin-2(A) A representative western blot & densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, & the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) & 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta –actin to ensure equal loading. (B) A representative western blot & densitometric analysis performed as in (A) shown here for Caco-2 cells. (C, D) Representative confocal microscopy immunofluorescence images from cells transfected with a scrambled control siRNA or 15-PGDH siRNA with or without stimulation with 40 nM LTC4 for 48 h. The expression of Mucin-2 (in red; antibody dilution, 1:500; DAPI in blue, dilution 1:1000) (C) in HT-29 cells & (D) Caco-2 cells is shown. The objective used was 63x, & the scale bar is 50 μm. The data are presented as the percent of control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416212329.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205129.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY S100A9 cDNA or the pCMV6-ENTRY control for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0007.jpg "Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY S100A9 cDNA or the pCMV6-ENTRY control for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.")
![Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89726-img0008.jpg)
![Immunohistochemistry-Paraffin: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200x magnification) of S100a9, in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 um. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2017.01774/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunohistochemistry-Paraffin-NB110-89726-img0010.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0006.jpg)
![Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/S100A9-Antibody-Simple-Western-NB110-89726-img0009.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415291935.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415371968.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415395938.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241539727.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241529199.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab administration on the azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated cancer development. (A) Experimental procedure of the control group & the AOM/DSS group treated with IgG Ab or anti-S100a9 Ab. (B) DAI of the IgG Ab or anti-S100a9 Ab-treated AOM/DSS mice & normal controls. (C) General observation of the colorectums in mice at the end of the 13th & 18th week. (D) Colon length, tumor rate, & number of macroscopic neoplasms were statisticed at 13 & 18 weeks, individually. n = 5–9 per group. Results were representative of the three experiments performed. (E) Histopathological examination of colon sections was shown under the Pannoramic Viewer (H&E staining, upper panels: original magnification 10×, scale bar: 2000 µm; lower panels: original magnification 200×, scale bar: 100 µm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205192.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab on the frequency of neutrophils, macrophages, & dendritic cells (DCs) in the colon of the dextran sulfate sodium (DSS) mouse model. (A) Colon lamina propria cells were isolated from normal control & IgG Ab or anti-S100a9 Ab-treated DSS mice at day 6 post-DSS colitis induction. Frequencies of neutrophils, macrophages, & DCs in the colon were determined by flow cytometry. Cells were gated on CD45+CD3−CD4−CD11b+Ly6G+, CD45+CD3−CD4−CD11b+F4/80+, & CD45+CD3−CD4−CD11b+CD11c+ populations respectively. Representative flow cytometric figures were shown. The percentage of cells was presented as the mean ± SEM of four to six individual mice per group. *p < 0.05 in a one-way analysis of variance followed by Bonferroni correction. Data were representative of three independent experiments. (B) Immunohistochemical staining of myeloperoxidase (MPO), CD68, & CD11c proteins in the normal control & IgG Ab or anti-S100a9 Ab-treated colitis mice at day 6 (left panels: original magnification 40×, scale bar: 200 µm; right panels: original magnification 200×, scale bar: 50 µm). Staining scores were counted. One-way analysis of variance followed by Bonferroni correction. Results were representative of the three experiments performed. Error bars represent SD. (C) Expression of S100a9, Tnf alpha , Il1 beta , Il6, Il17a, Ifn gamma , Il12a, Il23a, Il4, & Il10 mRNA, as assessed by quantitative real-time PCR in normal control & IgG Ab, or anti-S100a9 Ab-treated colitis tissues. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205114.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241621842.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Host CP (S100A8/A9) is elevated in H. pylori infected stomach tissue.A) s100a8/s100a9 transcript abundance in RNA extracted from C57BL/6 mice infected with H. pylori PMSS1 or SS1 for 1, 2, or 3 months relative to uninfected animals as determined by real-time RT-PCR analysis. Points indicate mean relative units of transcript abundance +/− SEM (levels of s100a8 in PMSS1-infected mice compared to uninfected mice; 1 mo p = 0.0511; 2 mo p = 0.0432; 3 mo p = 0.0127; levels of s100a8 in SS1-infected mice compared to uninfected mice at 2 mo p = 0.0623 Student's t test). (B) Inflammation scores of H. pylori infected mice at 1, 2, & 3 months post infection. (C) s100a8/s100a9 transcript abundance in RNA extracted from gastric biopsies derived from human patients, which were either H. pylori-positive or H. pylori-negative (s100a8 p = 0.15; s100a9 *p = 0.05). Bars indicate mean relative units of transcript abundance +/− SEM. Each experimental group represents≥5 individuals (mice or human samples). D) Gastric samples derived from H. pylori PMSS1-infected WT mice or SS1-infected WT mice at 2 months post-infection were analyzed via immunohistochemistry using a polyclonal antibody to S100A9 (scale bars are 50 microns). E) Real-time RT-PCR was performed on gastric tissue to quantify s100a8 & s100a9 transcript abundance from WT (C57BL/6 mice) & IL-17RA-/- mice infected with PMSS1. Data represent relative units of transcript abundance +/− SEM in WT mice & IL-17RA-/- mice, *p = 0.0169 & p = 0.0143, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25330071), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205130.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205132.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205144.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0003.jpg "Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0006.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0001.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0004.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0005.jpg "Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.")
![Immunohistochemistry: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Immunohistochemistry-NB400-152-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cathepsin E Antibody [NB400-152] - The expression and methylation status of Cathepsin E gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) and C57BL/6 (B6) mice. mRNA expression of Cathepsin E in CD4+, CD8+, B cells and macrophages in B6 and MRL mice. ***p<0.001. Western blot analyses of Cathepsin E in CD4+, CD8+, B cells and macrophages in B6, MRL and mice. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-38809-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0007.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E on mouse stomach lysate.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0001.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - The expression & methylation status of Ctse gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) & C57BL/6 (B6) mice. (A) mRNA expression of Ctse in CD4+, CD8+, B cells & macrophages in B6 & MRL mice. ***p < 0.001. (B) Western blot analyses of CTSE in CD4+, CD8+, B cells & macrophages in B6, MRL & mice. (C) Methylated region expanding 583 bp in Ctse intron 1 in B6 mice demonstrated by UCSC Genome Browser. In MRL mice, 583 bp region is hypomethylated. Duplicate reads are indicated by arrows. (D) Nucleotide sequence within 583 bp flanking region of Ctse intron 1 in B6 mice. Among 13 CpGs (red & underlined), 11th CpG (boxed) includes mCGCG binding motif for Kaiso. After genomic DNAs were bisulphite converted, amplified by PCR using Primer F1 & R1, subcloned, & isolated clones sequenced. 98.6% of CpGs (71 out of 72 colonies) in B6 mice & 76.9% CpGs (50 of 65) in MRL mice were methylated. (E) The structure of Kaiso, SMRT (Silencing Mediator of Retinoic acid & Thyroid hormone receptors)/N-CoR (Nuclear hormone receptor Co-Repressor), & HDAC3 (Histone deacetylase 3) complex. BTB (for BR-C, ttk & bab), POZ (for Pox virus & Zinc finger) & C2H2 zinc-finger domains are shown. (F) DNA sequencing of bisulphite converted DNAs in Ctse intron 1 in B6 & MRL mice. Percentage of methylation of 11th CpG is shown (n = 5). ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30816218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-152_rabbit-polyclonal-cathepsin-e-antibody-310202416161819.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: IRF6 Antibody (2C10) [NBP2-00949] Analysis of extracts (35ug) from 9 different cell lines by using anti-IRF6 monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Western-Blot-NBP2-00949-img0013.jpg "Western Blot: IRF6 Antibody (2C10) [NBP2-00949] Analysis of extracts (35ug) from 9 different cell lines by using anti-IRF6 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: IRF6 Antibody (2C10) [NBP2-00949] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunocytochemistry-Immunofluorescence-NBP2-00949-img0012.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human tonsil using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0011.jpg)
![Flow Cytometry: IRF6 Antibody (2C10) [NBP2-00949] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Flow-Cytometry-NBP2-00949-img0001.jpg)
![Western Blot: IRF6 Antibody (2C10) [NBP2-00949] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Western-Blot-NBP2-00949-img0002.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0003.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0004.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0005.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human breast tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0006.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human colon tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0007.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human Kidney tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0008.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human pancreas tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0009.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody (2C10) [NBP2-00949] - Staining of paraffin-embedded Human prostate tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00949-img0010.jpg)
![Western Blot: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Western-Blot-NBP2-71044-img0002.jpg "Western Blot: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.")
![Immunocytochemistry/Immunofluorescence: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71044-img0004.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human prostate tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0012.jpg)
![Flow Cytometry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IRF6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71044-img0003.jpg)
![Western Blot: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IRF6 monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Western-Blot-NBP2-71044-img0001.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0005.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0006.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0007.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human breast tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0008.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human colon tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0009.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human Kidney tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0010.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human pancreas tissue using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0011.jpg)
![Immunohistochemistry: IRF6 Antibody (OTI2C10) - Azide and BSA Free [NBP2-71044] - Staining of paraffin-embedded Human tonsil using anti-IRF6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IRF6-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71044-img0013.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0008.jpg "Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0007.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0005.jpg)
![Flow Cytometry: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Flow-Cytometry-NBP2-67180-img0001.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0002.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0003.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0004.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Western-Blot-NBP2-45815-img0006.jpg "Western Blot: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.")
![Immunohistochemistry: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Immunohistochemistry-NBP2-45815-img0005.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Western-Blot-NBP2-45815-img0007.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Immunohistochemistry-NBP2-45815-img0002.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Immunohistochemistry-NBP2-45815-img0003.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Immunohistochemistry-NBP2-45815-img0004.jpg)
![Immunofluorescence: JNK2 Antibody (OTI1A1) [NBP2-45815] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Immunofluorescence-NBP2-45815-img0001.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Western-Blot-NBP2-71259-img0002.jpg "Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71259-img0007.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0006.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Western-Blot-NBP2-71259-img0001.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0003.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0004.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87061-img0009.jpg "Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis in human urinary bladder and skeletal muscle tissues. Corresponding HPGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0017.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0021.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0019.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0018.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0020.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-49383] - Staining in human breast and pancreas tissues using anti-IRF6 antibody. Corresponding IRF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49383-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-49383] - Staining in human breast and pancreas tissues using anti-IRF6 antibody. Corresponding IRF6 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: IRF6 Antibody [NBP2-49383] - Analysis using Anti-IRF6 antibody NBP2-49383 (A) shows similar pattern to independent antibody NBP2-55961 (B).](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP2-49383-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: IRF6 Antibody [NBP2-49383] - Staining of human cell line HaCaT shows localization to nucleus, cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49383-img0011.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-49383] - Staining of human breast shows high expression.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49383-img0008.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-49383] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49383-img0009.jpg)
