| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52661-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75902SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48365 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44856 | |||
| Azide and BSA Free | NBP2-34803-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45347 | |||
| Azide and BSA Free | NBP2-34420-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-49879-0.1mg | |||
![Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-288202412461675.jpg "Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29820241492635.jpg)
![Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-292024119237.jpg)
![Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29202416453215.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0007.jpg "Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Basiliximab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0005.jpg)
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0006.jpg)
![ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.](http://images.novusbio.com/fullsize/IL-10-Antibody-JES3-12G8-ELISA-NBP2-27577-img0001.jpg "ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.")
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formalin fixed rat spleen slices were were stained with a NBP2-75902 at 5 ug/ml. The anti-human CD25 antibody NBP2-75902 also shows some cross-reactivity with rat in IHC.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Immunohistochemistry-NBP2-75902-img0001.jpg "Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formalin fixed rat spleen slices were were stained with a NBP2-75902 at 5 ug/ml. The anti-human CD25 antibody NBP2-75902 also shows some cross-reactivity with rat in IHC.")
![Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75902 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. Ab00188 shows weak labelling of rat cells by IF.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Immunofluorescence-NBP2-75902-img0002.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free [NBP2-75902] - Human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4) and rat small intestines (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75902 at 1 ug/ml before being detected by a secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75902_human-monoclonal-cd25-il-2r-alpha-antibody-basiliximab-chimeric-74202522141245.png)
![Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - Azide and BSA Free [NBP2-47865] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (CR1/802).](http://images.novusbio.com/fullsize/CD35-Antibody-CR1-802-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47865-img0001.jpg "Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - Azide and BSA Free [NBP2-47865] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (CR1/802).")
![Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - Azide and BSA Free [NBP2-47865] - Human Tonsil stained with CD35 Monoclonal Antibody (CR1/802).](http://images.novusbio.com/fullsize/CD35-Antibody-CR1-802-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47865-img0002.jpg)
![Western Blot: CD45RB Antibody (SPM569) [NBP2-32938] - Western Blot Analysis of human spleen tissue lysates using CD45RB Antibody (SPM569).](http://images.novusbio.com/fullsize/CD45RB-Antibody-SPM569-Western-Blot-NBP2-32938-img0004.jpg "Western Blot: CD45RB Antibody (SPM569) [NBP2-32938] - Western Blot Analysis of human spleen tissue lysates using CD45RB Antibody (SPM569).")
![Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938] - Formalin-fixed, paraffin-embedded human lymphoma stained with CD45RB Monoclonal Antibody (SPM569)](http://images.novusbio.com/fullsize/CD45RB-Antibody-SPM569-Immunohistochemistry-Paraffin-NBP2-32938-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) [NBP2-32938] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45RB MAb (SPM569).](http://images.novusbio.com/fullsize/nbp2-32938_mouse-monoclonal-cd45rb-antibody-spm569-17720241003215.jpg)
![Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Western-Blot-NB600-325-img0002.jpg "Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33728-img0007.jpg "Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody [NBP1-33728] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunohistochemistry-Paraffin-NBP1-33728-img0008.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody [NBP1-33728] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IMP2/IGF2BP2 antibody (NBP1-33728) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33728_rabbit-polyclonal-imp2-igf2bp2-antibody-91020242001046.jpg)
![Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 antibody (SPM544).](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Western-Blot-NBP2-34373-img0003.jpg "Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 antibody (SPM544).")
![Immunohistochemistry-Paraffin: CD44 Antibody (SPM544) [NBP2-34373] - Formalin-fixed paraffin-embedded human tonsil stained with CD44 Monoclonal antibody (SPM544)](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Immunohistochemistry-Paraffin-NBP2-34373-img0001.jpg)
![Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (SPM544).](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Western-Blot-NBP2-34373-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Immunocytochemistry-Immunofluorescence-NBP2-49878-img0003.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot. Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - human Hodgkin's Lymphoma stained with CD15 Rabbit Monoclonal Antibody (FUT4/1478R).](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Immunohistochemistry-Paraffin-NBP2-49878-img0001.jpg)
![Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-FUT4-1478R-Flow-Cytometry-NBP2-49878-img0002.jpg)
![Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha V antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-Western-Blot-NBP2-16973-img0008.jpg "Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha V antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: Integrin alpha V/CD51 Antibody [NBP2-16973] - Human colon carcinoma, using Integrin alpha V antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-Immunohistochemistry-Paraffin-NBP2-16973-img0006.jpg)
![Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-Western-Blot-NBP2-16973-img0004.jpg)
![Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - Sample (30 ug of whole cell lysate) A: RD (Rhabdomyosarcoma) cell (Mock control) B: RD transfected scramble siRNA C: RD transfected ITGAV siRNA 8% SDS PAGE gel, diluted at 1:900.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-Western-Blot-NBP2-16973-img0005.jpg)