| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76648SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-37712 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71256 | |||
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)
![Western Blot: TRAF-6 Antibody [NB100-56179] - Analysis of TRAF-6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-6 degradation fragments.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NB100-56179-img0011.jpg "Western Blot: TRAF-6 Antibody [NB100-56179] - Analysis of TRAF-6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-6 degradation fragments.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - Stained for TRAF6 using this antibody at 1:2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NB100-56179-img0008.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - 1:2000. Analysis Hematoxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NB100-56179-img0007.jpg)
![Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Western-Blot-NBP2-67639-img0007.jpg "Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.")
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of HUVEC cells labeling NLRP3/NAPL3 with Rabbit anti-NLRP3 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0011.jpg)
![Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Flow-Cytometry-NBP2-67639-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0006.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0004.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0010.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0023.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84682-img0012.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0016.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0022.jpg)
![Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Frozen-NBP1-84682-img0006.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0013.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0014.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420175183.gif "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419261860.gif)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in human brain tissue with Beclin-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0004.jpg)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in mouse brain tissue with Beclin-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin 1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419451274.gif)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Kidney Tissue.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120241958491.gif)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in HELA Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin 1 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419251598.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Human Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120242016947.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Mouse Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420115589.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Rat Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419592041.gif)
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Integrin-alpha-L-CD11a-Antibody-MEM-25-Flow-Cytometry-NB500-328-img0004.jpg "Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Analysis using the PE conjugate of NB500-328. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.](http://images.novusbio.com/fullsize/Integrin-alpha-L-CD11a-Antibody-MEM-25-Flow-Cytometry-NB500-328-img0002.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Staining pattern of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Integrin-alpha-L-CD11a-Antibody-MEM-25-Flow-Cytometry-NB500-328-img0003.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0015.jpg "Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: TRAF-6 Antibody [NBP1-80826] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80826-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0013.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0011.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Western-Blot-NBP1-48284-img0001.jpg "Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0008.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] - Immunofluorescent staining of HeLa cells using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Kidney tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0005.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Ovary tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0006.jpg)