| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36495SS | |||
| Azide and BSA Free | NBP2-80632 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64843SS | |||
| Azide and BSA Free | NBP2-80574 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-64979-0.05mg | |||
| Azide Free | NB100-64979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56334SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75294 | |||
| Biotin | NBP1-75295 | |||
| DyLight 350 | NBP1-76054 | |||
| DyLight 488 | NBP1-75299 | |||
| DyLight 550 | NBP1-76055 | |||
| DyLight 594 | NBP1-76056 | |||
| DyLight 633 | NBP1-76057 | |||
| DyLight 650 | NBP1-76058 | |||
| FITC | NBP1-75296 | |||
| HRP | NBP1-75297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-481SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43262-0.5mg | |||
| BSA Free | NBP1-43262-25ug | |||
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0004.jpg "Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/OS9-Antibody-Immunocytochemistry-Immunofluorescence-BC100-520-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0001.jpg)
![Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OS9-Antibody-Simple-Western-BC100-520-img0003.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482216.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483621.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482293.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483637.jpg)
![Western Blot: OS9 Antibody [BC100-520] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482250.jpg)
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) [NBP1-44861] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP1-44861) at 15 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Knockout-Validated-NBP1-44861-img0006.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) [NBP1-44861] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP1-44861) at 15 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0010.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0001.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0002.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) FITC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0003.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Flow Cytometry: CD81 Antibody (M38) [PE] [NBP1-44861PE] - A cell surface stain was performed on THP-1 cells with CD81 antibody (M38) NBP1-44861 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0004.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0005.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0007.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) biotin, streptavidin-APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0008.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0009.jpg)
![Flow Cytometry: TLR7 Antibody [NB100-56588] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary.](http://images.novusbio.com/fullsize/TLR7-Antibody-Flow-Cytometry-NB100-56588-img0001.jpg "Flow Cytometry: TLR7 Antibody [NB100-56588] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary.")
![Flow Cytometry: TLR7 Antibody [NB100-56588] - Analysis using the PE conjugate of NB100-56588. Staining of TLR7 in human PBMCs (monocytes) using 0.5 ug/10^6 cells of NBP2-24761. The shaded histogram represents cells alone. Green: PE-conjugated rabbit IgG isotype control (20304D). Red: TLR7 antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-Flow-Cytometry-NB100-56588-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR7 Antibody [NB100-56588] - Analysis of FFPE human brain using TLR7 antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Neuronal staining of TLR7 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TLR7-Antibody-Immunohistochemistry-Paraffin-NB100-56588-img0007.jpg)
![Flow (Intracellular): TLR7 Antibody [NB100-56588] - Analysis using the FITC conjugate of NB100-56588. Staining of TLR7 in TLR7/HEK 293 cells using this antibody at 2 ug/10^6 cells. Green: Vector/HEK 293 cells. Red: TLR7/HEK 293 cells.](http://images.novusbio.com/fullsize/TLR7-Antibody-Flow-Intracellular-NB100-56588-img0004.jpg)
![Western Blot: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of (A) partial recombinant 15 kDa human CD163 protein and (B) human lungs lysate with 2 ug/ml concentration of CD163 antibody clone 6D3.2F3](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Western-Blot-NBP2-36495-img0001.jpg "Western Blot: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of (A) partial recombinant 15 kDa human CD163 protein and (B) human lungs lysate with 2 ug/ml concentration of CD163 antibody clone 6D3.2F3")
![Immunohistochemistry-Paraffin: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of CD163 antibody (clone 6D3.2F3). CD163 is a histiocytic marker and in the tested placental tissue section, this antibody generated a specific staining in the Hofbauer cells which are eosinophilic histiocytes found in placenta.](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Immunohistochemistry-Paraffin-NBP2-36495-img0003.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of CD163 antibody (clone 6D3.2F3). CD163 is a histiocytic marker and in the tested placental tissue section, this antibody generated a specific staining in the Hofbauer cells which are eosinophilic histiocytes found in placenta.](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Immunohistochemistry-Paraffin-NBP2-36495-img0002.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Paraffin-NB100-64843-img0007.jpg "Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.")
![Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of mouse peripheral blood granulocytes with rat anti-mouse CD13.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Flow-Cytometry-NB100-64843-img0006.jpg)
![Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection anti-CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Frozen-NB100-64843-img0003.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of a mouse tail cryosection with anti-mouse CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Paraffin-NB100-64843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Immunocytochemistry-Immunofluorescence-NB100-64979-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.")
![Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Flow-Cytometry-NB100-64979-img0001.jpg)
![Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Western-Blot-NBP2-24904-img0003.jpg "Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: TLR3 [p Tyr759] Antibody [NBP2-24904] - Analysis of IgG, AID and TLR3 (A) and a hyper-IgM (HIGM) syndrome patient (B and C). A and B: Paraformaldehyde-fixed, frozen tonsillar mucosa tissue sections. C. Paraformaldehyde-fixed, mucosal B cells from the colon lamina propria of the HIGM patient. Arrowheads indicate cells co-expressing phospho TLR3, AID, and IgG. Data courtesy of W. Xu. et. al., and the Journal of Immunology](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24904-img0002.jpg)
![Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.](http://images.novusbio.com/fullsize/CCR1-Antibody-Western-Blot-NB100-56334-img0002.jpg "Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.")
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0008.jpg)
![Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Cytometry-NB100-56334-img0004.jpg)
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on U-87 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0007.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of Goat anti-Rabbit IgG (H+L) Secondary Antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Analysis of WNV-E in A549 cell lysate using Goat anti-Rabbit IgG (H+L) Secondary Antibody. The secondary antibody was diluted in 5% milk at a dilution of 1:3000. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0004.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0004.jpg "Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.")
![Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunohistochemistry-Frozen-NB100-481-img0005.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0007.jpg)
![Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunoprecipitation-NB100-481-img0006.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0020.jpg "Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Mouse (B7-H1) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0019.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0021.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0011.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0010.jpg)