| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56334SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-245SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56513SS | |||
| Azide and BSA Free | NBP2-33214 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37330-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59724SS | |||
| Azide and BSA Free | NBP2-80643 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90364-25ul | |||
![Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.](http://images.novusbio.com/fullsize/CCR1-Antibody-Western-Blot-NB100-56334-img0002.jpg "Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.")
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0008.jpg)
![Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Cytometry-NB100-56334-img0004.jpg)
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on U-87 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0007.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0009.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82086-img0014.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0010.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Western Blot analysis of various cells using NB100-82086 diluted at 1:1000](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-23420241545358.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-82086 incubated with blocking peptide (right)](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-103202523464543.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NB100-82086](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-11320251555922.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Lane 1: HEK293. Lane 2: Lentivirus mediated NFkB overexpression. We image submitted by a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-western-blot-1132025204015..jpg)
![Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Paraffin-NB600-1423-img0003.jpg "Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human")
![Flow Cytometry: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-Antibody-FA6-152-Flow-Cytometry-NB600-1423-img0007.jpg)
![Immunohistochemistry-Frozen: CD36 Antibody (FA6-152) [NB600-1423] - Frozen section of tonsil tissue. Staining especially in vascular endothelia.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Frozen-NB600-1423-img0001.jpg)
![Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse stained with FoxP3 and CD4.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-245-img0013.jpg "Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse stained with FoxP3 and CD4.")
![Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse incubated only with CD4 and no Foxp3.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-245-img0012.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-245] - Fox P3 was detected in immersion fixed paraffin-embedded sections of human tonsil using rabbit anti-human antibody (Catalog # NB600-245) at 1:100 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (Catalog # VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue).<br/>Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB600-245-img0011.jpg)
![Western Blot: FOXP3 Antibody [NB600-245] - Detection of human FOXP3 using NB 600-245. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector](http://images.novusbio.com/fullsize/FOXP3-Antibody-Western-Blot-NB600-245-img0009.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD8A and FOXP3 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB600-245-img0014.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD4 and FOXP3 antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB600-245-img0015.jpg)
![Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Western-Blot-NB100-56513-img0005.jpg "Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.")
![Immunohistochemistry-Frozen: IKK beta Antibody (10A9B6) [NB100-56513] - Mouse skin tissue section stained with IKK beta antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Immunohistochemistry-Frozen-NB100-56513-img0013.jpg)
![Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Flow-Intracellular-NB100-56513-img0011.jpg)
![Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Simple-Western-NB100-56513-img0010.jpg)
![Immunoprecipitation: IKK beta Antibody (10A9B6) [NB100-56513] - Antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Western-Blot-NB100-56513-img0012.jpg)
![Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mouse mAb against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Western-Blot-NBP2-37330-img0004.jpg "Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mouse mAb against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330] - CD90/Thy1 antibody (7E1B11) in indirect immunofluorescence staining of primary human fibroblasts using a red-fluorescent secondary antibody. Nuclei stained with DAPI (blue). Adjacent epithelial tumor cells stain negative for CD90. Image from verified customer review.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-37330-img0009.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - CD90 staining in human tonsil and aorta tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Immunohistochemistry-Paraffin-NBP2-37330-img0008.jpg)
![Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mAb against human THY1 recombinant protein. (Expected MW is 38.5 kDa).](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Western-Blot-NBP2-37330-img0002.jpg)
![Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mAb against HEK293 (1) and THY1 (AA: 17-132)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Western-Blot-NBP2-37330-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Immunofluorescence analysis of Hela cells using THY1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Immunocytochemistry-Immunofluorescence-NBP2-37330-img0005.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis of endometrial cancer tissues using THY1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Immunohistochemistry-NBP2-37330-img0006.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis of cerebellum tissues using THY1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-Immunohistochemistry-NBP2-37330-img0007.jpg)
![ELISA: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-7E1B11-ELISA-NBP2-37330-img0001.jpg)
![ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-ELISA-NB7343-img0001.jpg "ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.")
![Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (//waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-Dot-Blot-NB7343-img0002.jpg)
![Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Western-Blot-NB110-59724-img0007.jpg "Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.")
![Immunohistochemistry: CD36 Antibody (D-2712) [NB110-59724] - Staining of CD36 in mouse liver.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Immunohistochemistry-NB110-59724-img0006.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP1-90364-img0002.jpg "Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.")
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-90364_rabbit-polyclonal-creb-antibody-3052025726383.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Human foreskin cell lysate. CREB antibody dilution of 1:50. Protein concentration is 750 ug/mL. Detection is chemiluminescence. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0008.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Detection of CREB by Simple Western (JESS) in reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:50. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0009.jpg)
![Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NB600-242-img0010.jpg "Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry: FoxP3 Antibody [NB600-242] - (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-242-img0008.jpg)
![Immunohistochemistry: FoxP3 Antibody [NB600-242] - (8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NB600-242-img0012.jpg)
![Flow Cytometry: FoxP3 Antibody [NB600-242] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB600-242-img0009.jpg)
![Immunofluorescence: FoxP3 Antibody [NB600-242] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB600-242-img0011.jpg)
![Control: FoxP3 Antibody [NB600-242] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Control-NB600-242-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0015.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Staining (6ug/ml) of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0016.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NB100-2833] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NB100-2833-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0014.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia & neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), & neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) & 4 (NeuN) experimental mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-3102024153430.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241535694.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415363735.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - IL-1 beta synthesis, & microglial activation modulation in PDD005-treated aged mice. (A) Images showing IL-1 beta (green fluorescence), iba-1 (red fluorescence) expression. Yellow = Overlay; Blue = nuclei; SGZ region of mouse brain. 40X magnification, scale bar = 100 μm. (B) Graphs representing the average relative fluorescence of Iba-1 in SGZ. Significant differences determined by using one-way ANOVA with Tukey’s test. *P < 0.050, **P < 0.01 & ***P < 0.001 compared with aged-vehicle. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31980673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415304269.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415175271.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415171320.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415395951.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415284533.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation alters the expression of the alpha7 nicotinic acetylcholine receptor ( alpha 7nAchR) within microglia. (a) Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for alpha 7nAchR & Iba1. Images taken at 40X; scale bar = 25 µm (n = 6 mice/group). (b) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of alpha 7nAchR/Iba1 colocalization than the NonTg mice (p < .01). Additionally, we find a significant main effect of diet, where the Ch+ groups show a significant reduction in alpha 7nAchR/Iba1 colocalization than the CTL groups (p < .05). A genotype by diet interaction was found, where the APP/PS1 Ch+ mice show a significant reduction of alpha 7nR/Iba1 colocalization compared to the CTL counterparts (p < .001). The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. **p < .01, ***p < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415532058.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - (A–D) Images of coronal brain sections showing DiI (red) labeled EVs taken up by IBA1 (blue) & CD11b (green) positive microglia. (E,F) Tomato & control plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) & IBA1 (magenta) at P7, 10x (E), 20x (F). (G,H) 20x magnification of E showing CD11b (green) & IBA1 (red) (G) or CD11b (H). (I,J) Tomato & Rab27a shRNA plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) & IBA1 (magenta) at P7, 10x (I), 20x (J). (K,L) 20x magnification of I showing CD11b (green) & IBA1 (red) (K) or CD11b (L). (M) Western blot of Rab27a following shRNA mediated knockdown. (N) Images of coronal section stained for Rab27a (green) following control or Rab27a shRNA & tomato (magenta) co-electroporation at P0 & sacrificed 48 hrs later. (O) Quantification of CD11b positive microglia at P2 & P7 following control or Rab27a shRNA electroporation. Data are represented as mean ± SEM. ****p < 0.0001 A, E, I scale bar, 100 μm. B–D, F–H, J–L, N scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30816224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-3102024165742.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation reduces activated microglia. (a) Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for CD68 & Iba1. Images taken at 10X; Scale bar = 150 µm (n = 6 mice/group). Arrows illustrate colocalization of CD68/Iba1. (b) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of CD68/Iba1 colocalization than the NonTg mice (p < .05). Additionally, we find a significant main effect of diet, where the Ch+ groups show a significant reduction in CD68/Iba1 colocalization than the CTL groups (p < .001). Data are presented as box plots. The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. *p < .05, ***p < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415535148.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation alters the expression of the Sigma‐1 receptor ( sigma 1R) within microglia. (a‐b) Representative Western blot of sigma 1R levels. Quantitative analysis of sigma 1R protein levels reveals a significant reduction with Ch+ (p < .05; n = 5/APP group; n = 4/NonTg group). (c‐d) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of sigma 1R/Iba1 colocalization than the NonTg mice (p < .05, n = 6 APP/PS1 CTL, n = 5 APP/PS1 Ch+, n = 6 NonTg CTL, n = 6 NonTg Ch+). Additionally, we find a significant genotype by diet interaction where the APP/PS1 Ch+ mice show a significant reduction in sigma 1R/Iba1 colocalization than the APP/PS1 CTL mice (p < .001). Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for the sigma 1R & Iba1; images taken at 40×; scale bar = 25 µm. Data are presented as box plots. The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. *p < .05, ***p < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415535137.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NB100-2833] - Astrocytic marker was decreased in the brain of prenatally saline-exposed offspring, but not in the offspring prenatally exposed to LPS, following pharmacological activation of mGluR5.A prenatal administration of LPS did not change the brain expression level of iba1, CD68, GFAP or mGluR5 in 4-month-old mice (A). Postnatal CDPPB treatment reduced the GFAP level, without effect on iba1, CD68 or mGluR5 in the mice prenatally exposed to saline solution. The quantified molecular markers did not change in the brain of mice treated with MTEP (B). No change in iba1, CD68, GFAP or mGluR5 was observed in the brain of the mice prenatally exposed to LPS (C). Values are expressed as mean ± SEM. Abbreviations: MTEP, 3-((2-methyl-4-thiazolyl)ethynyl)pyridine; CDPPB, 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl) benzamide; CD68, cluster of differentiation 68); iba1, ionized calcium binding adaptor molecule-1; GFAP, glial fibrillary acidic protein; mGluR5, metabotropic glutamate receptor subtype 5; ROD, relative optical density. **p < 0.01 >> Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26536027), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241553740.jpg)