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![Dot Blot: Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7596] - Dot Blot of Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary antibody [PE] (Pre-adsorbed). Antigen: Mouse IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng or 1.23 ng as indicated.Primary antibody: N/A.Secondary antibody: Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary antibody [PE] (Pre-adsorbed) at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.](http://images.novusbio.com/fullsize/Donkey-F-ab-2-anti-Mouse-IgG-H+L-Secondary-Antibody-PE-Pre-adsorbed-Dot-Blot-NB7596-img0005.jpg "Dot Blot: Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7596] - Dot Blot of Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary antibody [PE] (Pre-adsorbed). Antigen: Mouse IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng or 1.23 ng as indicated.Primary antibody: N/A.Secondary antibody: Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary antibody [PE] (Pre-adsorbed) at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.")
![Dot Blot: Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7596] - Dot Blot results of Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary antibody [PE] (Pre-adsorbed).Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking buffer for 60 min at RT.Primary Antibody: Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin at 1ug/mL for 1hr at RT.Secondary Antibody: none.Imaged with ChemiDoc, Rhodamine filter.](http://images.novusbio.com/fullsize/Donkey-F-ab-2-anti-Mouse-IgG-H+L-Secondary-Antibody-PE-Pre-adsorbed-Dot-Blot-NB7596-img0006.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16026-img0007.jpg "Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CTGF Antibody [NBP2-16026] - Sample (30 ug of whole cell lysate) A: HUVEC 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CTGF-Antibody-Western-Blot-NBP2-16026-img0001.jpg)
![Western Blot: HLA DPA Antibody [NBP2-16851] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HLA DPA antibody (NBP2-16851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16851_rabbit-polyclonal-hla-dpa-antibody-910202420115074.jpg "Western Blot: HLA DPA Antibody [NBP2-16851] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HLA DPA antibody (NBP2-16851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HLA DPA Antibody [NBP2-16851] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HLA-DPA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16851-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DPA Antibody [NBP2-16851] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HLA-DPA-Antibody-Immunohistochemistry-Paraffin-NBP2-16851-img0002.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody [NBP2-23723] - Analysis of Latexin in paraffin-embedded formalin-fixed rat brain tissue using an isotype control (top, left) and NBP2-23723 (bottom, left and right (100X)) at 1:100.](http://images.novusbio.com/fullsize/Latexin-Antibody-Immunohistochemistry-Paraffin-NBP2-23723-img0002.jpg "Immunohistochemistry-Paraffin: Latexin Antibody [NBP2-23723] - Analysis of Latexin in paraffin-embedded formalin-fixed rat brain tissue using an isotype control (top, left) and NBP2-23723 (bottom, left and right (100X)) at 1:100.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0005.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 3 Antibody [NBP2-15356] - Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 ug of alpha Actinin 3 antibody. Western blot analysis was performed using alpha Actinin 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunoprecipitation-NBP2-15356-img0004.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15356 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15356_rabbit-polyclonal-alpha-actinin-3-antibody-9102024205628.jpg)
![Immunohistochemistry: JNK3 Antibody (29E10) [NBP3-26149] - Image of JNK3 Antibody (29E10) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26149_rabbit-jnk3-mab-29e10-2722024831370.jpg "Immunohistochemistry: JNK3 Antibody (29E10) [NBP3-26149] - Image of JNK3 Antibody (29E10) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: JNK3 Antibody (29E10) [NBP3-26149] - Positive Western Blot detected in: PC-3 whole cell lysate, Mouse brain tissue. All lanes: JNK3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 49, 32kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26149_rabbit-jnk3-mab-29e10-282202411472114.jpg)
![Immunocytochemistry/Immunofluorescence: JNK3 Antibody (29E10) [NBP3-26149] - Staining of PC-3 cell with JNK3 Antibody (29E10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 553-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26149_rabbit-jnk3-mab-29e10-282202412475137.jpg)
![Flow Cytometry: JNK3 Antibody (29E10) [NBP3-26149] - Overlay Peak curve showing PC3 cells stained with JNK3 Antibody (29E10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26149_rabbit-jnk3-mab-29e10-28220241431272.jpg)
![Western Blot: KIF1C Antibody [NB100-57510] - KIF1C is a novel HOOK3-interacting protein.sfGFP-3xFLAG and full length (FL) HOOK3, HOOK3 (1-552), and HOOK3 (553-718) all tagged with sfGFP and 3xFLAG were immunoprecipitated with alpha-FLAG antibodies from transiently transfected HEK-293T cells. Western blots with alpha-HC, alpha-FAM160A2, alpha-KIF1C, and alpha-FLAG antibodies were used to determine which proteins co-immunoprecipitated with each HOOK3 construct.DOI://dx.doi.org/10.7554/eLife.28257.015 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/28257), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Western-Blot-NB100-57510-img0002.jpg "Western Blot: KIF1C Antibody [NB100-57510] - KIF1C is a novel HOOK3-interacting protein.sfGFP-3xFLAG and full length (FL) HOOK3, HOOK3 (1-552), and HOOK3 (553-718) all tagged with sfGFP and 3xFLAG were immunoprecipitated with alpha-FLAG antibodies from transiently transfected HEK-293T cells. Western blots with alpha-HC, alpha-FAM160A2, alpha-KIF1C, and alpha-FLAG antibodies were used to determine which proteins co-immunoprecipitated with each HOOK3 construct.DOI://dx.doi.org/10.7554/eLife.28257.015 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/28257), licensed under a CC-BY license.")
![Immunoprecipitation: KIF1C Antibody [NB100-57510] - Detection of Human and Mouse KIF1C/LTXS1 on HeLa whole cell lysate using NB100-57510. KIF1C/LTXS1 was also IPed by rabbit anti-KIF1C/LTXS1 antibodies NB100-57507, NB100-57508 and NB100-57509.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunoprecipitation-NB100-57510-img0001.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-32897-img0005.jpg "Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.")
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-32897-img0007.jpg)
![HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIGR stained by PIGR antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10 μm.](http://images.novusbio.com/fullsize/antibody/nbp1-32897_rabbit-polyclonal-pigr-antibody-immunocytochemistry-immunofluorescence-273202392020.jpg)
![Human milk (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PIGR antibody [N2C1], Internal (NBP1-32897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/nbp1-32897_rabbit-polyclonal-pigr-antibody-western-blot-261120251849132.jpg)
![Western Blot: Granulysin Antibody [NBP2-27101] - Analysis of GNLY using HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Granulysin-Antibody-Western-Blot-NBP2-27101-img0001.jpg "Western Blot: Granulysin Antibody [NBP2-27101] - Analysis of GNLY using HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![ELISA: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Tested against purified Goat IgG F(c) (purple line), Goat IgG (green line). Each well was coated in duplicate with 1.0 ug. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay was performed using blocking buffer, donkey anti-Rabbit HRP and TMB ELISA Peroxidase substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-ELISA-NBP2-60668-img0001.jpg "ELISA: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Tested against purified Goat IgG F(c) (purple line), Goat IgG (green line). Each well was coated in duplicate with 1.0 ug. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay was performed using blocking buffer, donkey anti-Rabbit HRP and TMB ELISA Peroxidase substrate.")
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0006.jpg)
![Western Blot: GM-CSF Antibody [NBP2-27161] - Analysis of CSF2 in U937 cell lysate in the (1) absence and (2) presence of immunizing peptide using NBP2-27161 at 3 ug/ml. . Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GM-CSF-Antibody-Western-Blot-NBP2-27161-img0001.jpg "Western Blot: GM-CSF Antibody [NBP2-27161] - Analysis of CSF2 in U937 cell lysate in the (1) absence and (2) presence of immunizing peptide using NBP2-27161 at 3 ug/ml. . Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")