Rabbit anti-Goat IgG Fc Secondary Antibody [DyLight 680]

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Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG, Fc Antibody [DyLight 680] [NBP1-72834]

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 680
Concentration
LYOPH

Rabbit anti-Goat IgG Fc Secondary Antibody [DyLight 680] Summary

Immunogen
Goat IgG F(c) fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum.  No reaction was observed against Goat IgG F(ab')2.  This antibody will react with heavy chains of Goat IgG.  Minimal reactivity is expected against other Goat immunoglobulins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reactivity Notes

Reacts with Goat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 715, Excitation Wavelength: 682, Label Molecular Weight: 950, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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