| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54548-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76798SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25200SS | |||
| Azide and BSA Free | NBP2-80635 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80035-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80038-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37265-0.025ml | |||
![Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Western-Blot-NBP2-66763-img0007.jpg "Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC")
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0008.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0002.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0003.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0004.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0005.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0006.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse brain tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0009.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Knockout-Validated-NBP2-53318-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0006.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Knockout-Validated-NBP1-76798-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76798-img0002.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0013.jpg "Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.")
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Cell surface analysis of CD2 in 10^6 human lymphocytes using 0.25 ug of CD2 RPA-2.10 antibody; Shaded histogram represents cells without antibody; green represents isotype control ; red represents CD2 antibody. Goat anti-mouse IgG PE conjugated secondary was used in this test.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0001.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD2.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0008.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0009.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0010.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0011.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Immunocytochemistry-Immunofluorescence-DDX0130P-100-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.")
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma .](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Immunohistochemistry-Paraffin-DDX0130P-100-img0001.jpg)
![Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Western-Blot-NBP2-80035-img0003.jpg "Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).")
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) [NBP2-80035] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80035) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Immunohistochemistry-Paraffin-NBP2-80035-img0006.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0005.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0001.jpg)
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0003.jpg "Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).")
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 0.5 ug/mL sheep Fc-tagged recombinant human Spike S1 is shown to bind ACE-2 (black dotted line) detected by Donkey anti-Sheep APC Secondary Antibody (Catalog # F0127). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0004.jpg)
![Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Western-Blot-NBP2-80038-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0007.jpg)
![Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Flow-Cytometry-NBP2-80038-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0006.jpg)
![Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0001.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0009.jpg "Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.")
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0001.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0002.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0003.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0004.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0005.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0006.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0007.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunocytochemistry-Immunofluorescence-NBP2-37265-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0004.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of bladder cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Flow cytometric analysis of Hela cells using CD68 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Flow-Cytometry-NBP2-37265-img0006.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against human CD68 (AA: 42-155) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against HEK293 (1) and CD68 (AA: 42-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0003.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of endometrial cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0007.jpg)
![ELISA: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-ELISA-NBP2-37265-img0001.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)