| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-235SS | |||
| Azide and BSA Free | NBP2-80597 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97662-100ug | |||
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0028.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunohistochemistry-Paraffin-NB600-235-img0025.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-BSA-Free-Flow-Cytometry-NB600-235-img0032.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0027.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0011.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0013.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0015.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0024.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0029.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0030.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - The effect of IL-21 & CD40L exposure on MEC1 & MEC2 cells.Expression of EBNA-2 & LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) & LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 & upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 & Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes & 5×105 were loaded in both untreated & IL-21 treated MEC1 & MEC2 lanes. Note low expression of EBNA-2 & high expression of LMP-1 after IL-21 treatment & induction of Blimp-1 after IL-21 treatment. (C) Activity of the W & C promoters that regulate EBNA-2 expression & LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp & Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 & LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 & LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-235_mouse-monoclonal-blimp1-prdm1-antibody-3h2-e8-31020241616096.jpg)
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Western Blot: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Western-Blot-NBP2-75501-img0007.jpg "Western Blot: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Staining Galectin 8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Immunocytochemistry-Immunofluorescence-NBP2-75501-img0003.jpg)
![Immunohistochemistry-Paraffin: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of paraffin-embedded human colon cancer tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Immunohistochemistry-Paraffin-NBP2-75501-img0006.jpg)
![Flow Cytometry: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Flow-Cytometry-NBP2-75501-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Staining Galectin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Immunocytochemistry-Immunofluorescence-NBP2-75501-img0002.jpg)
![Immunohistochemistry-Paraffin: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Immunohistochemistry-Paraffin-NBP2-75501-img0004.jpg)
![Immunohistochemistry-Paraffin: Galectin-8 Antibody (JB85-35) [NBP2-75501] - Analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-JB85-35-Immunohistochemistry-Paraffin-NBP2-75501-img0005.jpg)
![Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0001.jpg)
![Western Blot: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Western-Blot-NBP2-76402-img0007.jpg "Western Blot: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate (observed molecular weight of ~ 57 KDa). Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402) and another rabbit anti-LAG3 recombinant monoclonal.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunoprecipitation-NBP2-76402-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunocytochemistry-NBP2-76402-img0002.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunocytochemistry-Immunofluorescence-NBP2-76402-img0010.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE tonsil by IHC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402), Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunohistochemistry-NBP2-76402-img0001.jpg)
![Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Western-Blot-H00011151-M01-img0007.jpg "Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody (4G10) [H00011151-M01] - Analysis of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Immunohistochemistry-Paraffin-H00011151-M01-img0005.jpg)
![Sandwich ELISA: Coronin-1a Antibody (4G10) [H00011151-M01] - Detection limit for recombinant GST tagged CORO1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Sandwich-ELISA-H00011151-M01-img0006.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP1-51991-img0001.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Flow-Cytometry-NBP1-51991-img0003.jpg)
![Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Immunofluorescence-NBP1-51991-img0002.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023922510.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023918332.jpg)
![Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB110-58867-img0003.jpg "Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58867] - Analysis of cortical neuron-glial cell culture from E20 rat stained with Coronin-1a Antibody, dilution 1:1,000 (Red), and costained with mouse mAb to GFAP, dilution 1:1,000 (Green). Hoechst staining of nuclear DNA (Blue). The Coronin-1a Antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58867-img0004.jpg)
![Western Blot: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Western-Blot-NBP2-76403-img0003.jpg "Western Blot: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate (observed molecular weight of ~ 57 KDa). Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) and rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunoprecipitation-NBP2-76403-img0004.jpg)
![Immunocytochemistry: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunocytochemistry-NBP2-76403-img0002.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR028F) [NBP2-76403] - Section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunohistochemistry-NBP2-76403-img0001.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0001.jpg "Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).")
![Western Blot: LAG-3 Antibody Pack [NBP3-11743] - Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated or treated (+) with 1 ug/mL PHA for 5 days and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LAG-3 at approximately 60-75 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Western-Blot-NBP3-11743-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 ug/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0003.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Analysis using the FITC conjugate of NBP1-97657. Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0005.jpg)
![Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-11E3-Flow-Cytometry-NBP1-97662-img0001.jpg "Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.")
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0002.jpg "Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71680-img0006.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0005.jpg)
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0001.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Coronin-1a antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0004.jpg)
![CyTOF-ready: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-CyTOF-ready-NBP2-71680-img0003.jpg)