| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-39474SS | |||
| Azide and BSA Free | NBP2-80562 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89546-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77030-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77047-0.025mg | |||
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Western-Blot-NBP1-39474-img0003.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0002.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0008.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0009.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0011.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0012.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0013.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin (APC).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0014.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0015.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89546-img0011.jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0015.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0012.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0013.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0014.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Simple Western lane view shows a specific band for FXR1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0006.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Electropherogram image(s) of corresponding Simple Western lane view. FXR1 antibody was used at 1:100 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0007.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Western-Blot-NBP1-47897-img0028.jpg "Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.")
![Immunocytochemistry/Immunofluorescence: NT-4 Antibody (2E8) [NBP1-47897] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunocytochemistry-Immunofluorescence-NBP1-47897-img0015.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0024.jpg)
![Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Western-Blot-NBP1-47897-img0027.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0016.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0017.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0018.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0020.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0022.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0008.jpg "Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0007.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0005.jpg)
![Flow Cytometry: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Flow-Cytometry-NBP2-67180-img0001.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0002.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0003.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0004.jpg)
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0012.jpg "Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0006.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0011.jpg)
![Immunohistochemistry: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunohistochemistry Validation of SCF/c-kit Ligand in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF/c-kit Ligand antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-2711202420441113.jpg)
![Immunocytochemistry/ Immunofluorescence: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunofluorescence Validation of SCF/c-kit Ligand in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF/c-kit Ligand with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-271120241928812.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunocytochemistry-immunofluorescence-8112023132213..jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132323..jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Mouse kidney.FXR1 stained by FXR1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132436..jpg)
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: zebrafish brain 7.5% SDS PAGE NBP1-33719 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33719_rabbit-polyclonal-fxr1-antibody-910202420134079.jpg)
![Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MKKS-Antibody-Western-Blot-NBP1-92123-img0006.jpg "Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: MKKS Antibody [NBP1-92123] - Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92123-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0012.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92123 (A) shows similar protein distribution across tissues to independent antibody NBP1-92122 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0009.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0010.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0011.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0013.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92123_rabbit-polyclonal-mkks-antibody-295202516101016.jpg)
![Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-212024940820.jpg "Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249425467.jpg)
![Western Blot: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249422617.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (1 ug/mL)Please notice that NBP3-25728 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518443414.jpg)
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (NBP3-25728) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518414124.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-western-blot-14112025184488.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (NBP3-25728) (5 ug/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (1 ug/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25728. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-141120251843335.jpg)
![Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Western-Blot-NBP2-45868-img0005.jpg "Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.")
![Immunohistochemistry-Paraffin: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Paraffin-embedded Carcinoma of Human prostate tissue using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-Paraffin-NBP2-45868-img0006.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0001.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0002.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0003.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0004.jpg)
![Western Blot: Neurturin Antibody [NBP1-77047] - HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.](http://images.novusbio.com/fullsize/Neurturin-Antibody-Western-Blot-NBP1-77047-img0001.jpg "Western Blot: Neurturin Antibody [NBP1-77047] - HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.")
![Immunocytochemistry/ Immunofluorescence: Neurturin Antibody - BSA Free [NBP1-77047] - Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 ug/mL.Green: Neuriturin Antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77047_rabbit-polyclonal-neurturin-antibody-2711202420175196.gif)
![Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77047_rabbit-polyclonal-neurturin-antibody-2711202419432247.gif)
![Immunocytochemistry/ Immunofluorescence: Neurturin Antibody - BSA Free [NBP1-77047] - Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml.Green: Neuriturin Antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77047_rabbit-polyclonal-neurturin-antibody-2711202420272445.gif)
![Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77047_rabbit-polyclonal-neurturin-antibody-2711202419562185.jpg)
![Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77047_rabbit-polyclonal-neurturin-antibody-271120242031295.gif)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0009.jpg "Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.")
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92122 (A) shows similar protein distribution across tissues to independent antibody NBP1-92123 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0006.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0007.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0010.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92122_rabbit-polyclonal-mkks-antibody-29520251625315.jpg)