| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87061-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87062-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70933 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88128-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34992-1mg | |||
| Carrier-Free | NBP2-34992-500ug | |||
![Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Simple-Western-NB200-179-img0005.jpg "Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) and patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: x250 (a, b), x100 (c, d). Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0015.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its expression. Double-immunofluorescence images show staining for 15-PGDH/HPGD-positive (red) cells and cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, and endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: x400. Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0016.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NB200-179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis . Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB200-179-img0003.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0009.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - A representative western blot and densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, and the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) and 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta-actin to ensure equal loading. Statistical analysis was conducted using an unpaired t-test; *P=0.05, **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD Antibody-Knockdown Validated-NB200-179-img0018.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291984.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415384117.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241539722.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291976.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241621232.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of LTC4 on 15-PGDH expression in the colon cancer cell line Caco-2(A) Quantification by qPCR of the mRNA expression of 15-PGDH following treatment with 40 nM LTC4 for 48 h in the presence or absence of AP100984 (a CysLT2 receptor antagonist, 1 μM) & JNK inhibitor I (10 μM). (B) Western blot showing 15-PGDH expression (antibody dilution, 1:5000) & densitometric analysis of LTC4-induced 15-PGDH up-regulation before or after the cells were stimulated with 40 nM LTC4 in the presence or absence of AP100984 & JNK inhibitor I for 48 h. (C) Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. The data are presented as the percent of untreated control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01, ***P<0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205174.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of 15-PGDH down-regulation on the cell differentiation markers SI & Mucin-2(A) A representative western blot & densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, & the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) & 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta –actin to ensure equal loading. (B) A representative western blot & densitometric analysis performed as in (A) shown here for Caco-2 cells. (C, D) Representative confocal microscopy immunofluorescence images from cells transfected with a scrambled control siRNA or 15-PGDH siRNA with or without stimulation with 40 nM LTC4 for 48 h. The expression of Mucin-2 (in red; antibody dilution, 1:500; DAPI in blue, dilution 1:1000) (C) in HT-29 cells & (D) Caco-2 cells is shown. The objective used was 63x, & the scale bar is 50 μm. The data are presented as the percent of control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416212329.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205129.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0004.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Flow-Cytometry-NBP2-66900-img0001.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0002.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver carcinoma tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87061-img0009.jpg "Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis in human urinary bladder and skeletal muscle tissues. Corresponding HPGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0017.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0021.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0019.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0018.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88127-img0009.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Analysis in human lymph node and skeletal muscle tissues. Corresponding Integrin beta 2/CD18 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88127-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88127 (A) shows similar protein distribution across tissues to independent antibody NBP1-88128 (B).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88127-img0016.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88127-img0015.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88127-img0018.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88127-img0020.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human urinary bladder and cerebral cortex tissues using NBP1-87062 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human urinary bladder and cerebral cortex tissues using NBP1-87062 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human stomach shows moderate to strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0018.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0017.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0019.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP1-87062-img0014.jpg)
![Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Western-Blot-NBP2-00490-img0004.jpg "Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.")
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00490-img0006.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-15-PGDH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00490-img0005.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0001.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0002.jpg)
![Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Western-Blot-NBP2-70933-img0002.jpg "Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.")
![Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70933-img0006.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-15-PGDH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0005.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70933-img0007.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0003.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88128 (A) shows similar protein distribution across tissues to independent antibody NBP1-88127 (B).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88128-img0018.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88128 (A) shows similar protein distribution across tissues to independent antibody NBP1-88127 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88128-img0014.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88128-img0015.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88128-img0016.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-Immunohistochemistry-Paraffin-NBP1-88128-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0011.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.")
![Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunohistochemistry-Frozen-NB200-610-img0018.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0007.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0001.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0008.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0015.jpg)
![SDS-Page: Human IFN-gamma Protein [NBP2-34992]](http://images.novusbio.com/fullsize/Human-IFN-gamma-Protein-SDS-Page-NBP2-34992-img0001.jpg "SDS-Page: Human IFN-gamma Protein [NBP2-34992]")
![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0002.jpg "Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.")
![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0001.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NB100-1482-img0006.jpg "Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Liver. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB100-1482-img0007.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB100-1482-img0005.jpg)