| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23484SS | |||
| Azide and BSA Free | NBP2-81015 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24464SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74910 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13996-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37329-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13159SS | |||
![Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-05987-img0017.jpg "Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0019.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-05987-img0003.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0004.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody NBP1-05987 used for IP at 3 ug per reaction. ZEB1 was also immunoprecipitated by another rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1, NBP1-05987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP1-05987-img0013.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Lin28a & ETM-related protein expression changed after the knockdown of Zeb1 in mESCs, mEB, GC1, & P19. Western blotting demonstrated that E-cadherin protein expression increased & that Lin28a & Vimentin protein expression decreased in Zeb1-siRNA transfected mESC (panel a), mEB (panel b), GC1 (panel c), & P19 (panel d) cells. Each experiment was performed in triplicate. Abbreviations: NC, negative control; siRNA, small interfering RNA; & WT, wild-type. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202415382430.jpg)
![Immunohistochemistry: ZEB1 Antibody [NBP1-05987] - Immunohistochemical staining results of EMT regulators in HG‐PanIN: SNAIL, SLUG, TWIST1, & ZEB1. We categorized & defined three grades: grade 0, <10% positive staining; grade 1, 10%‐50% positive; grade 2, 50%< positive Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30791220), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202415291996.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-05987] - Location of Zeb1 proteins in mEC lines (mESC, mEB, GC1, & P19). Immunofluorescence analysis was used to determine localization of Zeb1 in mESC, mEB, GC1, & P19 using a Zeb1 antibody (red). Cell nuclei were labeled with Hoechst 33342 (blue). The scale for the measurement bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241539712.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Lin28a & ETM-related protein expression changed after the knockdown of Zeb1 in mESCs, mEB, GC1, & P19. Western blotting demonstrated that E-cadherin protein expression increased & that Lin28a & Vimentin protein expression decreased in Zeb1-siRNA transfected mESC (panel a), mEB (panel b), GC1 (panel c), & P19 (panel d) cells. Each experiment was performed in triplicate. Abbreviations: NC, negative control; siRNA, small interfering RNA; & WT, wild-type. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241529198.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Lin28a & ETM-related protein expression changed after the knockdown of Zeb1 in mESCs, mEB, GC1, & P19. Western blotting demonstrated that E-cadherin protein expression increased & that Lin28a & Vimentin protein expression decreased in Zeb1-siRNA transfected mESC (panel a), mEB (panel b), GC1 (panel c), & P19 (panel d) cells. Each experiment was performed in triplicate. Abbreviations: NC, negative control; siRNA, small interfering RNA; & WT, wild-type. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202415304286.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Zeb1 expression in mESCs, mEB, GC1, & P19. (a) Zeb1 mRNA expression detected by real-time qPCR in mESC, mEB, GC1, & P19 (P < 0.001, data presented as mean value ± SD). (b) Zeb1 protein expression examined by western blot in mESC, mEB, GC1, & P19. Each experiment was performed in triplicate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202415304288.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Lin28a & ETM-related protein expression changed after the knockdown of Zeb1 in mESCs, mEB, GC1, & P19. Western blotting demonstrated that E-cadherin protein expression increased & that Lin28a & Vimentin protein expression decreased in Zeb1-siRNA transfected mESC (panel a), mEB (panel b), GC1 (panel c), & P19 (panel d) cells. Each experiment was performed in triplicate. Abbreviations: NC, negative control; siRNA, small interfering RNA; & WT, wild-type. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35611144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241528450.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-05987] - Correlation of the expression of ZEB1 & IL‐6 & other cytokines in various cancers. (A) Correlation analysis of the expression of ZEB1 & cytokines in breast cancer cell lines using data obtained from the Cancer Cell Line Encyclopedia (CCLE). Breast cancer cell lines were selected for the analysis, & each dot represents a cell line. Affymetrix microarray probe IDs for IL6 & IL8 are shown after the gene symbols on the y‐axis. RMA, robust multiarray average. (B) Representative results obtained from the tissue array analysis in Fig. S3. The top panel shows a case that was positive for both nuclear ZEB1 staining (green) & whole‐cell IL‐6 staining (red); scores = 4. The bottom panel shows a case that was negative for both ZEB1 & IL‐6; scores = 1. The samples were counterstained with DAPI to show cell density in the spot. Original magnification: 20 ×. (C) Kaplan–Meier survival curves of breast & lung cancer patients obtained from a public meta‐analysis database & Kaplan–Meier plotter (Gyorffy et al., 2010, 2013). The probability of overall survival of patients as split by median IL6 & IL8 expression is shown. Red: IL6 & IL8 high expression group; black: IL6 & IL8 low expression group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28618162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241616091.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - SU5402, FGFR1 inhibitor, affects the EMT transcription factors.(A, B) ERK1/2 phosphorylation (p-ERK1/2) determined by immunoblotting in HSC-4 & OTC-04 treated for 30 min w/ 30 ng/ml FGF2 or 30 ng/ml FGF7 in presence of 10% FBS (A) & in TSU & HOC313 cells treated for 1 h w/ 30 ng/ml FGF2 or 30 μM SU5402 in absence of FBS (B). F2, FGF2; F7, FGF7; SU, SU5402. (C) We have previously reported that, after treatment w/ TGF-beta , NMuMG cells underwent EMT w/ the IIIc-isoform of FGFR1[17]. After NMuMG cells pretreated w/ TGF-beta transfected w/ mouse Fgfr1 siRNA or treated w/ SU5402, the cells further incubated in culture medium (CM) from TSU cells. SU, SU5402; siFR1, siRNA against mouse Fgfr1. (D)FGF2 mRNA levels determined by RT-qPCR analyses. The ratio of FGF2 mRNA to GAPDH mRNA in HSC-4 cells indicated as “1”. Each value represents the mean ± SD of triplicate determinations from a representative experiment. Similar results obtained in at least three independent experiments. (E) ERK1/2 phosphorylation (p-ERK1/2) in TSU & HOC313 cells monitored in presence of indicated concentration of SU5402 for 1 h under serum-free culture conditions, followed by immunoblot analysis. (F, G) Expression of indicated genes in TSU cells under serum-free culture conditions determined by RT-qPCR (F) & immunoblot (G) analyses, following treatment w/ 10 μM SU5402. Each value represents the mean ± SD of triplicate determinations from a representative experiment. Similar results obtained from at least three independent experiments. p values determined by Student’s t-test. **p < 0.01. (H) TSU cells treated w/ 10 μM U0126 in absence of FBS subjected to immunoblotting w/ the indicated antibodies. alpha -tubulin used as a loading control (A, B, C, E, G, & H). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31682640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241616017.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - SU5402, FGFR1 inhibitor, affects the EMT transcription factors.(A, B) ERK1/2 phosphorylation (p-ERK1/2) determined by immunoblotting in HSC-4 & OTC-04 treated for 30 min w/ 30 ng/ml FGF2 or 30 ng/ml FGF7 in presence of 10% FBS (A) & in TSU & HOC313 cells treated for 1 h w/ 30 ng/ml FGF2 or 30 μM SU5402 in absence of FBS (B). F2, FGF2; F7, FGF7; SU, SU5402. (C) We have previously reported that, after treatment w/ TGF-beta , NMuMG cells underwent EMT w/ the IIIc-isoform of FGFR1[17]. After NMuMG cells pretreated w/ TGF-beta transfected w/ mouse Fgfr1 siRNA or treated w/ SU5402, the cells further incubated in culture medium (CM) from TSU cells. SU, SU5402; siFR1, siRNA against mouse Fgfr1. (D)FGF2 mRNA levels determined by RT-qPCR analyses. The ratio of FGF2 mRNA to GAPDH mRNA in HSC-4 cells indicated as “1”. Each value represents the mean ± SD of triplicate determinations from a representative experiment. Similar results obtained in at least three independent experiments. (E) ERK1/2 phosphorylation (p-ERK1/2) in TSU & HOC313 cells monitored in presence of indicated concentration of SU5402 for 1 h under serum-free culture conditions, followed by immunoblot analysis. (F, G) Expression of indicated genes in TSU cells under serum-free culture conditions determined by RT-qPCR (F) & immunoblot (G) analyses, following treatment w/ 10 μM SU5402. Each value represents the mean ± SD of triplicate determinations from a representative experiment. Similar results obtained from at least three independent experiments. p values determined by Student’s t-test. **p < 0.01. (H) TSU cells treated w/ 10 μM U0126 in absence of FBS subjected to immunoblotting w/ the indicated antibodies. alpha -tubulin used as a loading control (A, B, C, E, G, & H). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31682640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241616067.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Vimentin & ZEB1 protein levels are regulated by OVOL1 & OVOL2 in a human SCC cell line. (A) Relative protein expression levels of OVOL1 & OVOL2 in A431 cells treated with control siRNA, OVOL1 siRNA, or OVOL2 siRNA for 48 h. (Left) Representative blot images & (right) relative expression levels calculated from three independent experiments. (B) Relative protein expression levels of vimentin, ZEB1, & E-cadherin in A431 cells treated with control siRNA, OVOL1 siRNA, or OVOL2 siRNA for 72 h. (Upper) representative blot images & (lower) relative protein expression levels calculated from three independent experiments. Mann–Whitney U-test; error bars represent mean ± standard deviation. Protein expressions are relative to those of beta -actin as a reference. The control siRNA value was set to 1. p-values < 0.05 were assumed to indicate a statistically significant difference; * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32106476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202416175281.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - PDGFR-alpha / beta promotes sarcoma CSC migration/invasion & anchorage-independent growth.a Western blot analysis of cell adhesion protein N-cadherin & EMT transcription factors Snail, Slug, & Zeb1 in human sarcoma cell lines grown as monolayers & as spheroids. beta -actin was used as the loading control. b Representative images under light microscopy of migration & invasion assays of human sarcoma cell lines grown as monolayers or as spheroids for 24–48 h. Graphs display the number of migrated or invasive cells per field. c Representative light microscopy images of forrmed colonies in soft agar assay of human sarcoma cell lines grown as monolayers or spheroids for 14–20 days. Graph displays the number colonies per field. d Representative images under light microscopy of migration & invasion assays of human sarcoma cell lines grown as monolayers or as spheroids. Spheroid cells were treated with imatinib or DMSO. e Western blot analysis of N-cadherin & EMT-regulating transcription factors in human sarcoma cell lines grown as spheroids & treated with imatinib or DMSO. Experiments in a & e were performed three times with similar results. Bars represent standard deviation. *p < 0.05 compared to Monolayers Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0059-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202416182642.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - FGFR1 siRNAs attenuate the malignant phenotypes of cancer cells.(A) mRNA from TSU cells transfected with siRNAs against FGFR1 (siFGFR1) were subjected to conventional RT-PCR to determine the levels of endogenous FGFR1. (B, C, D) After transfection with siFGFR1 in TSU cells, phosphorylation of ERK1/2 (p-ERK1/2) (B), ZEB1 levels (C), & cell morphology (D) were determined. (E) TSU cells transfected with siFGFR1 were subjected to immunofluorescence analyses. Low magnification, 40×; high magnification, 80×. (F, G, H, I) After either treatment with SU5402 or transfection with siFGFR1 in TSU & HOC313 cells, the number of cells & invasive properties were determined under serum-free culture conditions. (J & K) After transfection with siFGFR1c in TSU & HOC313 cells, invasive & migratory properties were determined under serum-free culture conditions in TSU & HOC313 cells, respectively. siNC, non-specific control siRNA. Each value represents the mean ± SD of triplicate determinations from a representative experiment. Similar results were obtained from at least three independent experiments. p values were determined by Student’s t-test. *p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31682640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202416175241.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - PDGFR-alpha / beta promotes sarcoma CSC migration/invasion & anchorage-independent growth.a Western blot analysis of cell adhesion protein N-cadherin & EMT transcription factors Snail, Slug, & Zeb1 in human sarcoma cell lines grown as monolayers & as spheroids. beta -actin was used as the loading control. b Representative images under light microscopy of migration & invasion assays of human sarcoma cell lines grown as monolayers or as spheroids for 24–48 h. Graphs display the number of migrated or invasive cells per field. c Representative light microscopy images of forrmed colonies in soft agar assay of human sarcoma cell lines grown as monolayers or spheroids for 14–20 days. Graph displays the number colonies per field. d Representative images under light microscopy of migration & invasion assays of human sarcoma cell lines grown as monolayers or as spheroids. Spheroid cells were treated with imatinib or DMSO. e Western blot analysis of N-cadherin & EMT-regulating transcription factors in human sarcoma cell lines grown as spheroids & treated with imatinib or DMSO. Experiments in a & e were performed three times with similar results. Bars represent standard deviation. *p < 0.05 compared to Monolayers Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0059-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-310202416163718.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Nav1.4 protein levels are decreased in muscles from mouse models of SMA. (A) Immunoblot analysis using muscle lysate from P2, P5, P9, & P21 wild type mice. Nav1.4 protein levels increase during postnatal muscle development & form the predominant sodium channel expressed in mature skeletal muscle. GAPDH served as a loading control (N = 3). (B) Representative immunoblot with quantification, showing a decrease in levels of sodium channel Nav1.4 & Nav1.5 in P5 Smn-/-;SMN2 hindlimb muscle compared with controls (N = 3). (C) Quantification of immunoblot analyses in P21 Smn2B/- & control hindlimb muscles revealed a decrease in Nav1.4 levels. Early in postnatal muscle development, the Nav1.5 sodium channel isoform is the most predominant. In P21 Smn2B/- mice, the protein levels of Nav1.5 are higher than in controls (N = 3). (D) The protein level of the Nav1.4 positive regulator, NF1, is not altered in muscles from P21 Smn2B/- mice. Similarly, no change was detected in the protein levels of the Nav1.4 repressor ZEB. (E) Expression of sodium channel Nav1.4 in control sham & denervated samples 1 & 7 days post-denervation was assessed by immunoblot (N = 3). A decrease in the levels of Nav1.4 in muscle was noted at 7 days post-denervation. *, P < 0.05; **, P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24119341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05987_rabbit-polyclonal-zeb1-antibody-31020241617522.jpg)
![Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - CAFs induced miR-33b downregulation and promoted the EMT phenotype in lung cancer cells. The protein expression levels of E-cadherin, Vimentin, ZEB1, Twist1 and Snail1 in the lung cancer cells co-cultured with CAFs were detected by immunoblotting, using GAPDH protein as the loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23082), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Western-Blot-NBP2-23484-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - CAFs induced miR-33b downregulation and promoted the EMT phenotype in lung cancer cells. The protein expression levels of E-cadherin, Vimentin, ZEB1, Twist1 and Snail1 in the lung cancer cells co-cultured with CAFs were detected by immunoblotting, using GAPDH protein as the loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23082), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunocytochemistry-Immunofluorescence-NBP2-23484-img0009.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunohistochemistry-NBP2-23484-img0006.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Western-Blot-NBP2-23484-img0013.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunohistochemistry-NBP2-23484-img0004.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0010.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0011.jpg)
![Western Blot: FAM3C Antibody [NBP2-24464] - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FAM3C-Antibody-NBP2-24464-img0012.jpg "Western Blot: FAM3C Antibody [NBP2-24464] - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of tissue section of mouse brain using FAM3C antibody at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0010.jpg)
![Western Blot: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human brain lysate (0.5 ug/ml) 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate (0.5 ug/ml), and 4) in rat brain lysate (0.5 ug/ml) using FAM3C antibody.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Western-Blot-NBP2-24464-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Staining of FAM3C using FAM3C antibody in normal human brain at 5 ug/ml (using control rabbit Ig for figure B).](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0006.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human skin using FAM3C antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0007.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis tissue section of mouse brain using FAM3C antibody Lot A1 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0011.jpg)
![Simple Western: FAM3C Antibody [NBP2-24464] - Simple Western lane view shows a specific band for FAM3C in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Simple-Western-NBP2-24464-img0005.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP2-20942] - ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20942_rabbit-polyclonal-zeb1-antibody-91020242001064.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP2-20942] - ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 um.")
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20942_rabbit-polyclonal-zeb1-antibody-western-blot-261120251836823.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20942_rabbit-polyclonal-zeb1-antibody-western-blot-261120251820017.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20942_rabbit-polyclonal-zeb1-antibody-western-blot-2611202518301215.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0007.jpg "Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).")
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0006.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0008.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0001.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0003.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0005.jpg)
![Western Blot: FAM3C Antibody [NBP2-13996] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/FAM3C-Antibody-Western-Blot-NBP2-13996-img0009.jpg "Western Blot: FAM3C Antibody [NBP2-13996] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: FAM3C Antibody [NBP2-13996] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13996-img0005.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human hippocampus shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0001.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0006.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining in human duodenum and skeletal muscle tissues using anti-FAM3C antibody. Corresponding FAM3C RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human cerebral cortex shows positivity in neurons.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0010.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human placenta shows positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0011.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg)
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg)
![Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GLIPR2-Antibody-Western-Blot-NBP2-47126-img0004.jpg "Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.](http://images.novusbio.com/fullsize/GLIPR2-Antibody-Immunohistochemistry-Paraffin-NBP2-47126-img0002.jpg)
![Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.](http://images.novusbio.com/fullsize/GLIPR2-Antibody-Immunohistochemistry-Paraffin-NBP2-47126-img0003.jpg)
![Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP2-13159-img0002.jpg "Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-NBP2-13159-img0001.jpg)
![Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Simple-Western-NBP2-13159-img0003.jpg)
![Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-28820241423310.jpg "Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg)
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)