| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16148-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03471-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37285-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89951-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18651-0.02mg | |||
![Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Western-Blot-NBP3-16148-img0002.jpg "Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: JNK1/2/3 Antibody [NBP3-16148] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 antibody (NBP3-16148). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (NBP3-16148) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Immunoprecipitation-NBP3-16148-img0003.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-5220251255553.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-522025125443.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511392252.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511361224.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutamine Synthetase Antibody-Western Blot-NBP3-03471-img0008.jpg "Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251628083.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251632149.jpg)
![Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against HEK293 (1) and PRKAG1 (AA: 230-331)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Western-Blot-NBP2-37285-img0003.jpg "Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against HEK293 (1) and PRKAG1 (AA: 230-331)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunofluorescence analysis of HepG2 cells using PRKAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Immunocytochemistry-Immunofluorescence-NBP2-37285-img0004.jpg)
![Immunohistochemistry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunohistochemical analysis of paraffin-embedded kidney tissues using PRKAG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Immunohistochemistry-NBP2-37285-img0007.jpg)
![Flow Cytometry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Flow cytometric analysis of HepG2 cells using PRKAG1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Flow-Cytometry-NBP2-37285-img0005.jpg)
![Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against human PRKAG1 (AA: 230-331) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Western-Blot-NBP2-37285-img0002.jpg)
![Immunohistochemistry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PRKAG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-Immunohistochemistry-NBP2-37285-img0006.jpg)
![ELISA: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/AMPK-gamma-1-Antibody-7H3E9-ELISA-NBP2-37285-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS .](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Western-Blot-NBP2-70764-img0002.jpg "Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS .")
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0007.jpg)
![Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of extracts (10ug) from 4 different cell lines by using anti-FOS monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Western-Blot-NBP2-70764-img0001.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0003.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0004.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0005.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0006.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0008.jpg)
![Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Western-Blot-NBP2-89951-img0005.jpg "Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0003.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Analysis of Human HSPA1A expression on HeLa cells. The cells were treated, stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Flow-Cytometry-NBP2-89951-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0002.jpg)
![Immunoprecipitation: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunoprecipitation-NBP2-89951-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89951 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89951_rabbit-monoclonal-hsp70-hspa1a-antibody-001-235202521525031.png)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Western-Blot-NBP2-67032-img0007.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Flow-Cytometry-NBP2-67032-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0001.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0002.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with triple-vessel CAD. Histological sections of a graft wall obtained from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting. VEGFR-3 (b) is shown to be expressed by immunohistochemical staining in smooth muscle cells of the tunica media (TM) and is strongly present in endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Immunohistochemistry-NBP1-18651-img0011.jpg "Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with triple-vessel CAD. Histological sections of a graft wall obtained from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting. VEGFR-3 (b) is shown to be expressed by immunohistochemical staining in smooth muscle cells of the tunica media (TM) and is strongly present in endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.")
![Western Blot: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Analysis using recombinant human soluble VEGF Receptor 3 as target.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Western-Blot-NBP1-18651-img0010.jpg)
![Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal lymphatic endothelial cells (HDLEC).](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Flow-Cytometry-NBP1-18651-img0007.jpg)
![Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). There is only a very weak expression of VEGFR-3/FLT4 detectable in the HUVECs but a strong one in the HDMVEC.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Flow-Cytometry-NBP1-18651-img0006.jpg)
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with double-vessel CAD showing immunohistochemical staining. VEGF-C (a) & VEGFR-3 (b) reactivity in a graft wall obtained from a 67-year-old patient who developed the early graft restenosis within 7 months after coronary artery bypass grafting (the same patient as mentioned in Fig. 6). VEGF-C is localized exclusively within individual smooth muscle cells of the tunica media (TM). Cells with positive VEGFR-3 expression are situated in endothelial cells of the tunica intima (TI). c Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18651_mouse-monoclonal-vegfr3-flt-4-antibody-9d9-310202415363714.jpg)
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Immunohistochemical staining of an internal thoracic artery & saphenous vein wall harvested from a patient with no evidence of luminal stenosis 12 months after CABG. Immunohistochemical staining of VEGF-C (a) & CAV2 (b) in an internal thoracic artery (ITA) graft & VEGFR-3 (c) in saphenous vein (SV) transplant harvested from a 65-year-old patient diagnosed with triple-vessel CAD. Note that lack of CAV2 expression within smooth muscle cells (SMC) in ITA wall is accompanied by VEGF-C positive expression in the same area. In SV tissue sample harvested from the same patient, no VEGRR-3 expression was found. d Negative control. TI tunica intima, TM tunica media, TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18651_mouse-monoclonal-vegfr3-flt-4-antibody-9d9-310202415394521.jpg)
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (6310) [NBP2-43629] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: COX4I1 protein stained by COX4I1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunocytochemistry-Immunofluorescence-NBP2-43629-img0001.jpg "Immunocytochemistry/Immunofluorescence: COX4 Antibody (6310) [NBP2-43629] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: COX4I1 protein stained by COX4I1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - Analysis of U87 xenograft. COX4 antibody [6310] dilution: 1:200.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunohistochemistry-Paraffin-NBP2-43629-img0005.jpg)
![Western Blot: COX4 Antibody (6310) [NBP2-43629] - Analysis of A. 50 ug rat muscle lysate/extract 12 % SDS-PAGE COX4 antibody [6310] dilution: 1:1000](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Western-Blot-NBP2-43629-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - Analysis of hepatoma. COX4 antibody [6310] dilution: 1:200.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunohistochemistry-Paraffin-NBP2-43629-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.COX4 stained by COX4 antibody [GT6310] (NBP2-43629) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43629_mouse-monoclonal-cox4-antibody-6310-9102024201576.jpg)
![Western Blot: COX4 Antibody (6310) [NBP2-43629] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (NBP2-43629) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43629_mouse-monoclonal-cox4-antibody-6310-91020242011468.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (NBP2-43629) diluted at 1:1500.](http://images.novusbio.com/fullsize/nbp2-43629_mouse-monoclonal-cox4-antibody-6310-western-blot-2611202518222015.jpg)