| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30328SS | |||
| Azide and BSA Free | NBP2-80712 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| Azide and BSA Free | NBP2-80765 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-530SS | |||
![Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB300-155-img0004.jpg "Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).")
![Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-NB300-155-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/OS9-Antibody-Immunocytochemistry-Immunofluorescence-BC100-520-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0001.jpg)
![Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OS9-Antibody-Simple-Western-BC100-520-img0003.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482216.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483621.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482293.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483637.jpg)
![Western Blot: OS9 Antibody [BC100-520] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482250.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![<b>Biological Strategies Validation. </b>Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg)
![KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot NB500-161-1) of this antibody. For blotting immunoprecipitated p300, NB500-161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-NB500-161-img0011.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Western-Blot-NBP1-30328-img0004.jpg "Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.")
![Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Immunohistochemistry-NBP1-30328-img0005.jpg)
![Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Lane view shows a specific band for EGLN1/PHD2 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Simple-Western-NBP1-30328-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Western-Blot-NBP2-75978-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Knockout-Validated-NBP2-75978-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunocytochemistry-Immunofluorescence-NBP2-75978-img0002.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) [NBP2-75978] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte (TM) HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunohistochemistry-Paraffin-NBP2-75978-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0007.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75978 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0008.jpg)
![Western Blot: Snail Antibody [NBP1-80022] - Host: Mouse. Target Name: SNAI1. Sample Tissue: Mouse Spleen. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP1-80022-img0007.jpg "Western Blot: Snail Antibody [NBP1-80022] - Host: Mouse. Target Name: SNAI1. Sample Tissue: Mouse Spleen. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: Snail Antibody [NBP1-80022] - Rabbit Anti-SNAI1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue. Observed Staining: Cytoplasm. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Ma](http://images.novusbio.com/fullsize/Snail-Antibody-Immunohistochemistry-Paraffin-NBP1-80022-img0006.jpg)
![Western Blot: Snail Antibody [NBP1-80022] - 721_B cell lysate, Antibody Titration: 1ug/ml](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP1-80022-img0002.jpg)
![Western Blot: Snail Antibody [NBP1-80022] - Antibody Titration: 1 ug/ml Human 721_B.](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP1-80022-img0004.jpg)
![Western Blot: Snail Antibody [NBP1-80022] - Antibody Titration: 1 ug/ml Human A549.](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP1-80022-img0005.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody [NBP1-80022] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Snail-Antibody-Immunohistochemistry-Paraffin-NBP1-80022-img0003.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)
![Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Western-Blot-NB100-530-img0009.jpg "Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - V6.5 mouse embryonic stem cells (cat# NBP1-41162) stained with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-530-img0008.jpg)
![Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Top: relative expression of CD36, GLUT2, & VEGFR2 in duodenum; n = 6–8. All values are expressed as model-based means, with error bars representing the confidence intervals (95% CI). *P value < 0.05. Bottom: Western blots of CD36 (75 kDa), GLUT2 (55 kDa), & VEGFR2 (105 kDa). Animals without a detectable band were excluded from the analysis. HIIT, high-intensity interval training; MICT, moderate-intensity continuous training; CON, control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28183816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-530_rabbit-polyclonal-vegfr2-kdr-flk-1-antibody-310202415334928.jpg)
