| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48494-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21480-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62557-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78133SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38661-25ul | |||
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human testis shows no in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0009.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0008.jpg)
![Western Blot: Survivin Antibody [NBP2-48494] - Western blot using [NBP2-48494]. Analysis in control (vector only transfected HEK293T lysate) and BIRC5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells). Note: predicted molecular weight of survivin is 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NBP2-48494-img0008.jpg "Western Blot: Survivin Antibody [NBP2-48494] - Western blot using [NBP2-48494]. Analysis in control (vector only transfected HEK293T lysate) and BIRC5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells). Note: predicted molecular weight of survivin is 16 kDa.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Immunohistochemical analysis in formalin-fixed paraffin-embedded human testis and skeletal muscle tissues using Survivin Antibody [NBP2-48494]. Corresponding BIRC5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0007.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human skeletal muscle using Survivin Antibody [NBP2-48494] shows no nuclear positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0003.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human skin using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0004.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human placenta using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0005.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NBP2-48494] - Staining of formalin-fixed paraffin-embedded human testis using Survivin Antibody [NBP2-48494] shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NBP2-48494-img0006.jpg)
![Western Blot: Frizzled-8 Antibody [NB100-2439] - Staining of human pancreas lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Frizzled-8-Antibody-Western-Blot-NB100-2439-img0004.jpg "Western Blot: Frizzled-8 Antibody [NB100-2439] - Staining of human pancreas lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NB100-2439] - Staining of paraffin embedded Human Pancreas. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-detection.](http://images.novusbio.com/fullsize/Frizzled-8-Antibody-Immunohistochemistry-Paraffin-NB100-2439-img0001.jpg)
![Immunohistochemistry-Paraffin: CELSR2 Antibody [NLS1943] - Anti-CELSR2 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CELSR2-Antibody-Immunohistochemistry-Paraffin-NLS1943-img0004.jpg "Immunohistochemistry-Paraffin: CELSR2 Antibody [NLS1943] - Anti-CELSR2 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CELSR2 Antibody [NLS1943] - Analysis of anti-CELSR2 antibody with human, colon, epithelium.](http://images.novusbio.com/fullsize/CELSR2-Antibody-Immunohistochemistry-Paraffin-NLS1943-img0001.jpg)
![Immunohistochemistry-Paraffin: CELSR2 Antibody [NLS1943] - Analysis of anti-CELSR2 antibody with human brain, cortex, neurons at dilution 7 ug/ml.](http://images.novusbio.com/fullsize/CELSR2-Antibody-Immunohistochemistry-Paraffin-NLS1943-img0003.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Notch-1-Antibody-E6-Chimeric-Flow-Cytometry-NBP2-62557-img0002.jpg "Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62557_rabbit-monoclonal-notch-1-antibody-e6-chimeric-74202522141235.png)
![Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-Western-Blot-NBP2-24611-img0002.jpg "Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27370-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27370-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DAB1 Antibody [NBP2-16095] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DAB1 protein stained by DAB1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16095-img0006.jpg "Immunocytochemistry/Immunofluorescence: DAB1 Antibody [NBP2-16095] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DAB1 protein stained by DAB1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-16095] - Mouse duodenum. DAB1 antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-16095-img0004.jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dab1 antibody [N1N3] (NBP2-16095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16095_rabbit-polyclonal-dab1-antibody-western-blot-2611202518463718.jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dab1 antibody [N1N3] (NBP2-16095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp2-16095_rabbit-polyclonal-dab1-antibody-western-blot-261120251827021.jpg)
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Western-Blot-NBP2-78133-img0002.jpg "Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NBP2-78133-img0001.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Analysis in human small intestine and placenta tissues using NBP2-38661 antibody. Corresponding DAB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Analysis in human small intestine and placenta tissues using NBP2-38661 antibody. Corresponding DAB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DAB1 Antibody [NBP2-38661] - Staining of human cell line RT4 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38661-img0002.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human small intestine shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0007.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0008.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0009.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0010.jpg)