| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18543-100ul | |||
| BSA Free | NBP3-18543-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95089-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44715 | |||
| Azide and BSA Free | NBP2-34519-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48440 | |||
| Azide and BSA Free | NBP3-11434 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21678-50ul | |||
![Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP3-05535-img0002.jpg "Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP3-05535] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with GAP-43 Antibody at a dilution of 1:1,000, in red, and costained with chicken pAb to MAP2 at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP-43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05535-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0004.jpg "Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-69990] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-NBP2-69990-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0003.jpg)
![Control: CTGF/CCN2 Antibody [NBP2-69990] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Control-NBP2-69990-img0001.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241943225.jpg "Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunofluorescence of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241928876.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Western blot analysis of Calcium-sensing R/CaSR in EL4 cell lysate with Calcium-sensing R/CaSR antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120242092044.gif)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0005.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human prostate using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0001.jpg)
![Immunoprecipitation: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of 200ug extracts of LO2 cells, using Sodium Potassium ATPase Alpha 1 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunoprecipitation-NBP2-95089-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Immunohistochemistry-Paraffin-NBP2-34280-img0003.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) or stained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5 + 123A8) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Flow-Cytometry-NBP2-34280-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Analysis using the Azide and BSA Free version of NBP2-34280. Staining of human colon ganglion stained with CD56 MAb (123C3.D5+123A8).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Immunohistochemistry-Paraffin-NBP2-34280-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (135-4C5) [NBP2-44863] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45 antibody (135-4C5) [NBP2-44863] followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/CD45-Antibody-135-4C5-Immunocytochemistry-Immunofluorescence-NBP2-44863-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD45 Antibody (135-4C5) [NBP2-44863] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45 antibody (135-4C5) [NBP2-44863] followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: CD45 Antibody (135-4C5) [NBP2-44863] - Human Tonsil stained with CD45 antibody (135-4C5) [NBP2-44863].](http://images.novusbio.com/fullsize/CD45-Antibody-135-4C5-Immunohistochemistry-Paraffin-NBP2-44863-img0001.jpg)
![Flow Cytometry: CD45 Antibody (135-4C5) [NBP2-44863] - Flow cytometry analysis of lymphocyte gated PBMCs unstained (gray) or stained with CD45 antibody (135-4C5) (green).](http://images.novusbio.com/fullsize/CD45-Antibody-135-4C5-Flow-Cytometry-NBP2-44863-img0004.jpg)
![Flow Cytometry: CD45 Antibody (135-4C5) [NBP2-44863] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 antibody (135-4C5) [NBP2-44863] followed by Goat anti-Mouse-IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45-Antibody-135-4C5-Flow-Cytometry-NBP2-44863-img0002.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Western-Blot-NB100-2032-img0006.jpg "Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.")
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NB100-2032-img0003.jpg)
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NB100-2032-img0002.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231526123.jpg "Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.")
![Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-57202317161873.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231558486.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0011.jpg "Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.")
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in PC-12.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0008.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in IMR-32.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0009.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in Raw 264.7.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0010.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10 Analysis of YWHAG expression in K-562.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0012.jpg)
![ELISA: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Sandwich-ELISA-H00007532-M02-img0007.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)