| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95255-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34628-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43281-0.1mg | |||
| BSA Free | NBP1-43281-0.05mg | |||
| Azide and BSA Free | NBP2-80573 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44488 | |||
| BSA Free | NBP2-52868-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90208-25ul | |||
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0008.jpg "Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfection control at 6 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfection control.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0001.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfected cells expressing Frizzled-6 at 6 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with human pancreas.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0006.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0007.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunocytochemistry-Immunofluorescence-NBP3-16352-img0002.jpg "Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunohistochemistry of paraffin-embedded human colon using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunohistochemistry-Paraffin-NBP3-16352-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of U-2 OS cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251215490.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of NIH/3T3 cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251242529.jpg)
![Western Blot: sFRP-4 Antibody (7Z6Q1) [sFRP-4] - Western blot analysis of various lysates using sFRP-4 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-52202517192946.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Sodium Potassium ATPase Alpha 1 Antibody-Western Blot-NBP2-95255-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded mouse kidney using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human liver cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human lung cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody - Azide and BSA Free [Sodium Potassium ATPase Alpha 1] - Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp2-95255_rabbit-polyclonal-sodium-potassium-atpase-alpha-1-antibody-5220251640221.jpg)
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)
![Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-24771-img0002.jpg "Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.")
![Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Detection of Aminopeptidase A/ENPEP in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse CD11b (Catalog # FAB1124P) and Rat Anti-Mouse Aminopeptidase A/ENPEP Monoclonal Antibody (Catalog # NBP1-43281, top panel) or isotype control antibody (Catalog # MAB006, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/Aminopeptidase-A-ENPEP-Antibody-6C3-Flow-Cytometry-NBP1-43281-img0003.jpg "Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Detection of Aminopeptidase A/ENPEP in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse CD11b (Catalog # FAB1124P) and Rat Anti-Mouse Aminopeptidase A/ENPEP Monoclonal Antibody (Catalog # NBP1-43281, top panel) or isotype control antibody (Catalog # MAB006, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Staining of BALB/c bone marrow cells with 0.5 ug of Rat IgG2a K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse BP-1 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used](http://images.novusbio.com/fullsize/Aminopeptidase-A-ENPEP-Antibody-6C3-Flow-Cytometry-NBP1-43281-img0002.jpg)
![Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/SRGAP1-Antibody-5D2-Western-Blot-H00057522-M03-img0002.jpg "Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in NIH/3T3 ( Cat # L018V1 ).")
![Immunohistochemistry-Paraffin: SRGAP1 Antibody (5D2) [H00057522-M03] - Analysis of monoclonal antibody to SRGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SRGAP1-Antibody-5D2-Immunohistochemistry-Paraffin-H00057522-M03-img0005.jpg)
![Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in IMR-32 ( Cat # L008V1 ).](http://images.novusbio.com/fullsize/SRGAP1-Antibody-5D2-Western-Blot-H00057522-M03-img0001.jpg)
![ELISA: SRGAP1 Antibody (5D2) [H00057522-M03] - Detection limit for recombinant GST tagged SRGAP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SRGAP1-Antibody-5D2-ELISA-H00057522-M03-img0004.jpg)
![Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/DAAM1-Antibody-4H3-Western-Blot-H00023002-M03-img0006.jpg "Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).")
![Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/DAAM1-Antibody-4H3-Western-Blot-H00023002-M03-img0005.jpg)
![Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3. Lane 1: DAAM1 transfected lysatE (122.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DAAM1-Antibody-4H3-Western-Blot-H00023002-M03-img0007.jpg)
![ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DAAM1-Antibody-4H3-ELISA-H00023002-M03-img0003.jpg)
![Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/DAAM1-Antibody-4H3-Proximity-Ligation-Assay-H00023002-M03-img0004.jpg)
![Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-288202412442995.jpg "Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)
![Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Western-Blot-NBP2-52868-img0006.jpg "Western Blot: CD45 Antibody (CL0159) [NBP2-52868] - Western Blot analysis using CD45 antibody (CL0159) [NBP2-52868]. Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate. Observed molecular weight ~200 kDa.")
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human tonsil with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human cortex using CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in microglia.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-NBP2-52868-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human liver with CD45 antibody (CL0159) [NBP2-52868] shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human lung with CD45 antibody (CL0159) [NBP2-52868] shows strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0159) [NBP2-52868] - Staining of human small intestine with CD45 antibody (CL0159) [NBP2-52868] shows strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0159-Immunohistochemistry-Paraffin-NBP2-52868-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0012.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human lymph node shows no cytoplasmic positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0011.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0010.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0013.jpg)