| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40765SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89756-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89804-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77027-0.025mg | |||
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71771-img0008.jpg "Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: GABARAP Antibody [NBP1-71771] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunohistochemistry-NBP1-71771-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - GABARAP antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71771-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40765-img0003.jpg "Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40765-img0004.jpg)
![Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Simple-Western-NB110-40765-img0005.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar protein distribution across tissues to independent antibody NBP1-89758 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar protein distribution across tissues to independent antibody NBP1-89758 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: GM130/GOLGA2 Antibody [NBP1-89756] - Analysis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar pattern to independent antibody NBP1-89758 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP1-89756-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89756-img0012.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89756.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0018.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0007.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0008.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0015.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human testis.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0016.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human colon.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0017.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis using Anti-G6PD antibody NBP1-89805 (A) shows similar pattern to independent antibody NBP1-89804 (B).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89805-img0021.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis using Anti-G6PD antibody NBP1-89805 (A) shows similar pattern to independent antibody NBP1-89804 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89805-img0006.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0020.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis in human cell lines A-549 and HEK293 using anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89805-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining in human testis and skeletal muscle tissues.. Corresponding G6PD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0016.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0017.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0018.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human testis and skeletal muscle tissues. Corresponding G6PD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human testis and skeletal muscle tissues. Corresponding G6PD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human cell lines A-549 and HEK293 using anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89804-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis using Anti-G6PD antibody NBP1-89804 (A) shows similar pattern to independent antibody NBP1-89805 (B).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89804-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89804-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0021.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0017.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human spleen shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0018.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human colon shows moderate cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0019.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human liver shows moderate to strong cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with at 20mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202420312949.gif "Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with at 20mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).")
![Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Human Liver.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202419241330.jpg)
![Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody at 10 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202419392785.jpg)
![Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Rat Liver.Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-271120242045617.jpg)
![Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Rat Liver. Loading: 10 ug of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202420285845.gif)
![Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Human Liver.Loading: 10 ug of lysates per lane.Antibodies: Albumin, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202419392736.gif)
![Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Loading: 10 µg of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77027_chicken-polyclonal-albumin-antibody-2711202419293848.gif)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0002.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0004.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70454-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0003.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunoprecipitation (IP) of CRYAB by using a monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70454-img0007.jpg)