| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78403SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23659SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87160-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21356-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91654-25ul | |||
![Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR17.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunocytochemistry-Immunofluorescence-NLS4229-img0003.jpg "Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR17.")
![Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunohistochemistry-Paraffin-NLS4229-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunocytochemistry-Immunofluorescence-NLS4229-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - IHC staining of human brain, neurons and glia.](http://images.novusbio.com/fullsize/GPR17-Antibody-Immunohistochemistry-Paraffin-NLS4229-img0001.jpg)
![Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/GCC185-Antibody-Western-Blot-NBP2-04024-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-04024-img0004.jpg)
![Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/5-HT4-Antibody-Western-Blot-NBP1-78403-img0003.jpg "Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/5-HT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78403-img0004.jpg)
![Western Blot: Frizzled-10 Antibody [NBP2-23659] - Analysis of Frizzled-10 in human brain lysate. Lane 1 shows pre-immune sera and Lanes 2 show NBP2-23659 tested on human at 1:1000 dilution.](http://images.novusbio.com/fullsize/Frizzled-10-Antibody-Western-Blot-NBP2-23659-img0001.jpg "Western Blot: Frizzled-10 Antibody [NBP2-23659] - Analysis of Frizzled-10 in human brain lysate. Lane 1 shows pre-immune sera and Lanes 2 show NBP2-23659 tested on human at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: LPAR3/LPA3/EDG-7 Antibody [NBP3-27861] - Immunohistochemical analysis of paraffin-embedded human colon cancer](http://images.novusbio.com/fullsize/nbp3-27861_rabbit-lpar3-lpa3-edg-7-pab-75202410133444.jpg "Immunohistochemistry-Paraffin: LPAR3/LPA3/EDG-7 Antibody [NBP3-27861] - Immunohistochemical analysis of paraffin-embedded human colon cancer")
![Western Blot: LPAR3/LPA3/EDG-7 Antibody [NBP3-27861] - Western Blot analysis of 3T3 cells](http://images.novusbio.com/fullsize/nbp3-27861_rabbit-lpar3-lpa3-edg-7-pab-75202410133416.jpg)
![Immunocytochemistry/ Immunofluorescence: LPAR3/LPA3/EDG-7 Antibody [NBP3-27861] - Immunofluorescence analysis of A549 cells. The picture on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/nbp3-27861_rabbit-lpar3-lpa3-edg-7-pab-752024939131.jpg)
![Western Blot: FFAR2/GPR43 Antibody [NBP3-12190] - WB of NBP3-12190 with mouse pancreas. 1:500 antibody dilution in DiluObuffer. Apparent MW is 39 kDa.](http://images.novusbio.com/fullsize/FFAR2-GPR43-Antibody-Western-Blot-NBP3-12190-img0001.jpg "Western Blot: FFAR2/GPR43 Antibody [NBP3-12190] - WB of NBP3-12190 with mouse pancreas. 1:500 antibody dilution in DiluObuffer. Apparent MW is 39 kDa.")
![Immunohistochemistry-Paraffin: FFAR2/GPR43 Antibody [NBP3-12190] - 1:100 dilution in IHC blobking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000.FFPE section.](http://images.novusbio.com/fullsize/FFAR2-GPR43-Antibody-Immunohistochemistry-Paraffin-NBP3-12190-img0002.jpg)
![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Orexin-R2-HCRTR2-Antibody-1E3-Western-Blot-H00003062-M01-img0006.jpg "Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCRTR2 monoclonal antibody (M01), clone 1E3 (Cat # H00003062-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Orexin-R2-HCRTR2-Antibody-1E3-Western-Blot-H00003062-M01-img0004.jpg)
![ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Detection limit for recombinant GST tagged HCRTR2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Orexin-R2-HCRTR2-Antibody-1E3-ELISA-H00003062-M01-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: GPR50 Antibody [H00009248-B01P] - Analysis of negative control 293 cells (Black) and GPR50 expressing 293 cells (Green) using GPR50 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/GPR50-Antibody-Flow-Cytometry-H00009248-B01P-img0003.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - Analysis of GPR50 expression in transfected 293T cell line by GPR50 polyclonal antibody. Lane 1: GPR50 transfected lysate(67.87 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0004.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482257.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482280.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Analysis in human cerebral cortex and liver tissues. Corresponding GPRC5B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Analysis in human cerebral cortex and liver tissues. Corresponding GPRC5B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GPRC5B Antibody [NBP1-87160] - Staining of human cell line U-251 MG shows localization to nucleus and vesicles.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87160-img0012.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human caudate putamen shows strong membranous positivity in glial cells.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0019.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0015.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human kidney shows strong membranous positivity in cells in glomeruli and distal tubules.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0016.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human liver shows weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0017.jpg)
![Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GPRC5B-Antibody-Immunohistochemistry-Paraffin-NBP1-87160-img0018.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21356_rabbit-tdag8-gpr65-pab-4520231548529.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: AGTRAP Antibody [NBP1-91654] - Staining of human cell line A-431 shows positivity in vesicles.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91654-img0005.jpg "Immunocytochemistry/Immunofluorescence: AGTRAP Antibody [NBP1-91654] - Staining of human cell line A-431 shows positivity in vesicles.")
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0010.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0002.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Analysis in human epididymis and skeletal muscle tissues using NBP1-91654 antibody. Corresponding AGTRAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0006.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Epididymis shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0007.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0008.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0009.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0011.jpg)