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![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0007.jpg "Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0004.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS,OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cellsprepared using NETN lysis buffer. Antibody: Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP3-14687] - Whole cell lysate(1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody. For blotting immunoprecipitated E-Cadherin, was used at 1:1000.Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP3-14687-img0001.jpg)
![Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Western-Blot-NBP2-72740-img0009.jpg "Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Anti-MMP13 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72740-img0008.jpg)
![Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of extracts (10ug) from 5 different cell lines by using anti-MMP13 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Western-Blot-NBP2-72740-img0001.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72740-img0007.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0010.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0011.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0012.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0013.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0015.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0002.jpg "Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0008.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0007.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0006.jpg)
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0008.jpg "Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Knockout-Validated-NBP3-15842-img0001.jpg)
![Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Immunoprecipitation-NBP3-15842-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284937.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517184515.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517162378.jpg)
![Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223995.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517202968.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-522025171893.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223986.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517172874.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-5220251718979.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517273553.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284993.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-6F9G8-Knockout-Validated-NBP3-15654-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311049.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517352755.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517304949.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-522025173458.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517344234.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311076.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517361268.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311081.jpg)
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa")
![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Western-Blot-NBP2-15188-img0018.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.7).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.7). Formalin-paraffin human Angiosarcoma.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Immunohistochemistry-Paraffin-NBP2-15188-img0012.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometric Analysis of paraformaldehyde-fixed Jurat cells using CD31/PECAM-1 Antibody (C31.7)followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0017.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using the Azide and BSA Free version of CD31/PECAM-1 Antibody (C31.7). Detection of CD31 in THP-1 lysate.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Western-Blot-NBP2-15188-img0014.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometric Analysis of human CD31 on Jurkat cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0015.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0016.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0009.jpg "Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Usage of Beta Catenin antibody in ICC of HT-29 cells](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunocytochemistry-NBP2-67307-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0010.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: HT29 Lane 3: A431](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0007.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0008.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse small intestine tissue using anti-b-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0006.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0009.jpg "Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0008.jpg)
![Flow Cytometry: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Flow-Cytometry-NBP2-67650-img0001.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0005.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining in human placenta and tonsil tissues using anti-LAMC1 antibody. Corresponding LAMC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining in human placenta and tonsil tissues using anti-LAMC1 antibody. Corresponding LAMC1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human cerebral cortex, kidney, placenta and tonsil using Anti-LAMC1 antibody NBP1-87718 (A) shows similar protein distribution across tissues to independent antibody NBP1-87719 (B).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Laminin gamma 1 Antibody [NBP1-87718] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Western-Blot-NBP1-87718-img0014.jpg)
![Western Blot: Laminin gamma 1 Antibody [NBP1-87718] - Analysis in mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Western-Blot-NBP1-87718-img0015.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human tonsil using Anti-LAMC1 antibody NBP1-87718.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0017.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0008.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0009.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0012.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human kidney.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0013.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human placenta using Anti-LAMC1 antibody NBP1-87718.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0016.jpg)
![Simple Western: Laminin gamma 1 Antibody [NBP1-87718] - Simple Western lane view shows a specific band for LAMC1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Simple-Western-NBP1-87718-img0005.jpg)
![Simple Western: Laminin gamma 1 Antibody [NBP1-87718] - Electropherogram image(s) of corresponding Simple Western lane view. Laminin gamma 1 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Simple-Western-NBP1-87718-img0006.jpg)