| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52589-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
![Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NBP1-33105-img0007.jpg "Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MCM4 Antibody [NBP1-33105] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33105-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-33105-img0004.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - MCM4 antibody detects MCM4 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug NCI-H929 whole cell lysate/extract. 7.5 % SDS-PAGE. MCM4 antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NBP1-33105-img0006.jpg)
![Immunoprecipitation: MCM4 Antibody [NBP1-33105] - MCM4 antibody immunoprecipitates MCM4 protein in IP experiments. IP samples: Jurkat whole cell extract. A. 40 ug Jurkat whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM4 protein by 4 ug MCM4 antibody. 7.5 % SDS-PAGE. The immunoprecipitated MCM4 protein was detected by MCM4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunoprecipitation-NBP1-33105-img0008.jpg)
![Immunohistochemistry: MCM4 Antibody [NBP1-33105] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using MCM4 antibody (NBP1-33105) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-91020242041276.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM4 Antibody [NBP1-33105] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-910202420134039.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-33105 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-91020242010442.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-657] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-657) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-657-img0003.jpg "Western Blot: DNA-PKcs Antibody [NB100-657] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-657) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Western Blot: DNA-PKcs Antibody [NB100-657] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-657. DNA-PKcs antibodies NB100-658, NB100-659 and NB100-660.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-657-img0002.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Western-Blot-NBP2-02004-img0014.jpg "Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-02004-img0013.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human tonsil using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0012.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATRIP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0003.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Western-Blot-NBP2-02004-img0004.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0005.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0006.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human breast tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0007.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human Kidney tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0008.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human liver tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0009.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human pancreas tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0010.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human prostate tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0011.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Hela cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0001.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Jurkat cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0002.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Western-Blot-NBP2-72271-img0002.jpg "Western Blot: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP.")
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human pancreas tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0013.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72271-img0006.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Western-Blot-NBP2-72271-img0001.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human breast tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0007.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human tonsil using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0008.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0009.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0010.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human Kidney tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0011.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human liver tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0012.jpg)
![Immunohistochemistry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Staining of paraffin-embedded Human prostate tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72271-img0014.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Analysis of Hela cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72271-img0003.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - Analysis of Jurkat cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72271-img0004.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) - Azide and BSA Free [NBP2-72271] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ATRIP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI5E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72271-img0005.jpg)
![Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School](http://images.novusbio.com/fullsize/Rad51D-Antibody-5B3-6-Western-Blot-NB100-178-img0003.jpg "Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School")
![Immunocytochemistry/Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Rad51D-Antibody-5B3-6-Immunocytochemistry-Immunofluorescence-NB100-178-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Western Blot: Rad50 Antibody [NBP2-20054] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal diluted at 1:5000.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20054-img0007.jpg "Western Blot: Rad50 Antibody [NBP2-20054] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal diluted at 1:5000.")
![Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20054-img0002.jpg)
![Western Blot: RAD50 Antibody [NBP2-20054] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20054-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rad50 Antibody [NBP2-20054] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20054-img0003.jpg)
![Western Blot: Rad50 Antibody [NBP2-20054] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal diluted at 1:1000.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20054-img0004.jpg)
![Immunoprecipitation: Rad50 Antibody [NBP2-20054] - NT2D1 whole cell extract A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody [C1C2], Internal 5 % SDS-PAGE The immunoprecipitated Rad50 protein was detected by Rad50 antibody [C1C2], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Rad50-Antibody-Immunoprecipitation-NBP2-20054-img0006.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunocytochemistry-Immunofluorescence-NBP3-13664-img0003.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunohistochemistry-Paraffin-NBP3-13664-img0002.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0001.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0004.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0005.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0006.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242011411.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-910202420115086.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242021972.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-1610202419405743.jpg)
![Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Western-Blot-NBP3-14678-img0002.jpg "Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.")
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human HeLa cells treated with etoposide. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunocytochemistry-Immunofluorescence-NBP3-14678-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human breast carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:100. Secondary: DyLight(R) 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0007.jpg)
![Flow Cytometry: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Rabbit Histone H2AX antibody (BLR053F) or isotype control (unshaded). Secondary: DyLight(R) 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Flow-Cytometry-NBP3-14678-img0008.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human basal cell carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of mouse CT26 tumor. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0005.jpg)
![Immunoprecipitation: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-gamma-H2AX recombinant monoclonal antibody used for IP at 20 ul per reaction. gamma-H2AX was also immunoprecipitated by rabbit anti-gamma-H2AX antibody. For blotting immunoprecipitated gamma-H2AX, was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower panel: Rabbit anti-H2AX](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-Immunoprecipitation-NBP3-14678-img0001.jpg)
![Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Western-Blot-NBP2-52589-img0008.jpg "Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Immunofluorescence-NBP2-52589-img0002.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of paraffin-embedded endometrial cancer tissues using XRCC6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Immunohistochemistry-Paraffin-NBP2-52589-img0004.jpg)
![Flow Cytometry: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Flow-Cytometry-NBP2-52589-img0001.jpg)
![Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Western-Blot-NBP2-52589-img0006.jpg)
![Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Western-Blot-NBP2-52589-img0005.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of paraffin-embedded cervical cancer tissues using XRCC6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-Immunohistochemistry-Paraffin-NBP2-52589-img0003.jpg)
![ELISA: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-2F7F5-ELISA-NBP2-52589-img0009.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Western-Blot-NBP3-15378-img0004.jpg "Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Mouse brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Rat brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2AX Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517192975.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223915.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251718450.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517184545.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251720298.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223963.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517273511.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517284912.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251717282.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Western blot analysis of various lysates using Histone H2AX Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517152711.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)