| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89463-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-2566SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89524-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83350-25ul | |||
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/XRCC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89463-img0012.jpg "Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Analysis in human testis and liver tissues using NBP1-89463 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/XRCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-89463-img0016.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249374979.jpg "Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Non-transfected (-) and transfected (+) PP2A alpha shRNA-transfected 293T (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024941484.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249342754.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024935555.jpg)
![Immunocytochemistry/Immunofluorescence: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249362724.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249413318.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024938310.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249403842.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249374958.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16182-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP2-16182-img0002.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0001.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -Rat colon.DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunohistochemistry-paraffin-992024123530..jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunocytochemistry-immunofluorescence-992024123725..jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-910202420134047.jpg)
![Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-91020242041277.jpg)
![Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Western-Blot-NBP3-15780-img0006.jpg "Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Knockout-Validated-NBP3-15780-img0001.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517311064.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304910.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517315592.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [HDAC1] - Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733513.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304961.jpg)
![Immunoprecipitation: HDAC1 Antibody (2L6O7) [HDAC1] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 0.5ug HDAC1 antibody . Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733586.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304925.jpg)
![Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-pp15-IB4-Western-Blot-NB100-189-img0004.jpg "Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.")
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Analysis of a FFPE tissue section of the human breast cancer using 1:200 dilution of BRIP1/FANCJ antibody (NB100-189). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-pp15-IB4-Immunohistochemistry-Paraffin-NB100-189-img0005.jpg)
![Western Blot: FANCG Antibody [NB100-2566] - Detection of FANC G in transfected COS1 cell lysate.](http://images.novusbio.com/fullsize/FANCG-Antibody-Western-Blot-NB100-2566-img0002.jpg "Western Blot: FANCG Antibody [NB100-2566] - Detection of FANC G in transfected COS1 cell lysate.")
![Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - XRCC5 monoclonal antibody (M02), clone 3D8. Analysis of XRCC5 expression in A-431.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-3D8-Western-Blot-H00007520-M02-img0008.jpg "Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - XRCC5 monoclonal antibody (M02), clone 3D8. Analysis of XRCC5 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-3D8-Immunocytochemistry-Immunofluorescence-H00007520-M02-img0004.jpg)
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-3D8-Immunohistochemistry-Paraffin-H00007520-M02-img0006.jpg)
![Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of XRCC5 expression in transfected 293T cell line by XRCC5 monoclonal antibody (M02), clone 3D8.Lane 1: XRCC5 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-3D8-Western-Blot-H00007520-M02-img0009.jpg)
![Sandwich ELISA: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Detection limit for recombinant GST tagged XRCC5 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-3D8-Sandwich-ELISA-H00007520-M02-img0007.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89524-img0007.jpg "Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0014.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Tonsil shows moderate nuclear positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83350-img0008.jpg "Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
