| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38954-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38567-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85728-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06705SS | |||
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0012.jpg "Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunocytochemistry-Immunofluorescence-H00005347-M01-img0007.jpg)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0010.jpg)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0011.jpg)
![Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Proximity-Ligation-Assay-H00005347-M01-img0005.jpg)
![Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunoprecipitation-H00005347-M01-img0008.jpg)
![ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-ELISA-H00005347-M01-img0013.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0014.jpg "Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunocytochemistry-Immunofluorescence-H00003065-M06-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunohistochemistry-Paraffin-H00003065-M06-img0009.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0010.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0011.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0012.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0013.jpg)
![ELISA: HDAC1 Antibody (1D6) [H00003065-M06] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-ELISA-H00003065-M06-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0002.jpg "Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0001.jpg)
![Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Western-Blot-NBP2-88933-img0008.jpg "Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunocytochemistry-Immunofluorescence-NBP2-88933-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human human embryonal carcinoma of testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis cancer, seminoma shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0006.jpg)
![Western Blot: Ku70/XRCC6 Antibody [NBP2-38954] - Analysis using Anti-XRCC6 antibody NBP2-38954 (A) shows similar pattern to independent antibody NBP2-38567 (B).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Western-Blot-NBP2-38954-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Ku70/XRCC6 Antibody [NBP2-38954] - Analysis using Anti-XRCC6 antibody NBP2-38954 (A) shows similar pattern to independent antibody NBP2-38567 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38954] - Staining of human cerebral cortex, kidney, skin and testis using Anti-XRCC6 antibody NBP2-38954 (A) shows similar protein distribution across tissues to independent antibody NBP2-38567 (B).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38954-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-38954] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38954-img0004.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38954] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38954-img0014.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38954] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38954-img0011.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38954] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38954-img0012.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38954] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38954-img0013.jpg)
![Western Blot: Ku70/XRCC6 Antibody [NBP2-38567] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Western-Blot-NBP2-38567-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Ku70/XRCC6 Antibody [NBP2-38567] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human cerebral cortex, kidney, skin and testis using Anti-XRCC6 antibody NBP2-38567 (A) shows similar protein distribution across tissues to independent antibody NBP2-38954 (B).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-38567] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38567-img0004.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0015.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0012.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0013.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human pancreas shows low positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0013.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0015.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-8L6B6-Immunohistochemistry-Paraffin-NBP3-15358-img0001.jpg "Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Analysis of lysates from A-549 cells using Ku70 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-western-blot-281202510168..png)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -Confocal imaging of U-2 OS cells using Ku70 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-immunocytochemistry-immunofluorescence-2812025101725..png)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -Analysis of paraffin-embedded Human tonsil tissue using Ku70 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-immunohistochemistry-paraffin-2812025101846..png)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -Analysis of paraffin-embedded Human esophagus tissue using Ku70 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-immunohistochemistry-paraffin-2812025101940..png)
![Immunohistochemistry: Ku70/XRCC6 Antibody (8L6B6) [Ku70/XRCC6] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Ku70/XRCC6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-52202517172894.jpg)
![Immunohistochemistry: Ku70/XRCC6 Antibody (8L6B6) [Ku70/XRCC6] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Ku70/XRCC6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-5220251726056.jpg)
![Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [Ku70/XRCC6] - Confocal imaging of U-2 OS cells using Ku70/XRCC6 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-52202517172838.jpg)
![Immunohistochemistry: Ku70/XRCC6 Antibody (8L6B6) [Ku70/XRCC6] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Ku70/XRCC6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-52202517162386.jpg)
![Western Blot: Ku70/XRCC6 Antibody (8L6B6) [Ku70/XRCC6] - Western blot analysis of lysates from A-549 cells using Ku70/XRCC6 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15358_rabbit-ku70-xrcc6-mab-8l6b6-52202517251955.jpg)
![Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06705-img0004.jpg "Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06705] - Staining of paraffin-embedded mouse skin with Nbs1 Antibody [NBP1-06705].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06705-img0003.jpg)
![Western Blot: PP2A alpha Antibody [NBP1-31266] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP1-31266-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PP2A alpha Antibody [NBP1-31266] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody [NBP1-31266] - Paraformaldehyde-fixed HeLa, using PP2A alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31266-img0003.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP1-31266] - A549 xenograft, using PPP2CA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Immunohistochemistry-Paraffin-NBP1-31266-img0004.jpg)
![Western Blot: PPP2R4 Antibody [NBP1-31266] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Western-Blot-NBP1-31266-img0005.jpg)
![Western Blot: PPP2R4 Antibody [NBP1-31266] - Sample (30 ug of whole cell lysate) A: 293T B: Molt-4 10% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Western-Blot-NBP1-31266-img0006.jpg)
![Immunoprecipitation: PP2A alpha Antibody [NBP1-31266] - Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 ug of PP2A alpha antibody Western blot analysis was performed using PP2A alpha antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunoprecipitation-NBP1-31266-img0011.jpg)
![Western Blot: Rad50 Antibody [NBP2-20055] - Rad50 antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody. 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by Rad50 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0007.jpg "Western Blot: Rad50 Antibody [NBP2-20055] - Rad50 antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody. 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by Rad50 antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20055-img0003.jpg)
![Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0001.jpg)
![Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0004.jpg)
![Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0005.jpg)
![Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0006.jpg)