| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-599SS | |||
| Azide and BSA Free | NBP2-80601 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54677-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85728-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38567-25ul | |||
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20054-img0002.jpg "Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: RAD50 Antibody [NBP2-20054] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20054-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rad50 Antibody [NBP2-20054] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20054-img0003.jpg)
![Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20054_rabbit-polyclonal-rad50-antibody-western-blot-261120251819362.jpg)
![Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-20054_rabbit-polyclonal-rad50-antibody-western-blot-261120251821106.jpg)
![ELISA: Universal 8-oxo-dG ELISA Kit (Colorimetric) [NBP3-00370] - Samples were spiked with high concentrations of 8-oxo-dG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00370_universal-8-ohdg-elisa-kit-colorimetric-13220241692288.jpg "ELISA: Universal 8-oxo-dG ELISA Kit (Colorimetric) [NBP3-00370] - Samples were spiked with high concentrations of 8-oxo-dG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Universal 8-oxo-dG ELISA Kit (Colorimetric) [NBP3-00370] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00370_universal-8-ohdg-elisa-kit-colorimetric-432024955051.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunocytochemistry-Immunofluorescence-NBP3-13664-img0003.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunohistochemistry-Paraffin-NBP3-13664-img0002.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0004.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0005.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0006.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat tissues.</br>53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242011411.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse tissues.</br>53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-910202420115086.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242021972.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-1610202419405743.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-western-blot-2611202518303816.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Western-Blot-NBP3-15378-img0004.jpg "Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Mouse brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Rat brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2AX Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517192975.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223915.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251718450.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517184545.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251720298.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223963.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517273511.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517284912.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251717282.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Western blot analysis of various lysates using Histone H2AX Rabbit mAb at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. <br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517152711.jpg)
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0008.jpg "Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (KEN) [NB100-599] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BRCA1 (KEN) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Immunocytochemistry-Immunofluorescence-NB100-599-img0009.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0005.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0014.jpg "Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunocytochemistry-Immunofluorescence-H00003065-M06-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunohistochemistry-Paraffin-H00003065-M06-img0009.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0010.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0011.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0012.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0013.jpg)
![ELISA: HDAC1 Antibody (1D6) [H00003065-M06] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-ELISA-H00003065-M06-img0007.jpg)
![Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Western-Blot-NBP2-88933-img0008.jpg "Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunocytochemistry-Immunofluorescence-NBP2-88933-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human human embryonal carcinoma of testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis cancer, seminoma shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0006.jpg)
![Western Blot: 53BP1 Antibody [NBP2-54677] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. The observed molecular weight as seen on this gel as greater than 250 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NBP2-54677-img0007.jpg "Western Blot: 53BP1 Antibody [NBP2-54677] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. The observed molecular weight as seen on this gel as greater than 250 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.")
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NBP2-54677] - Staining of human cell line A-431 with 53BP1 Antibody shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54677-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody [NBP2-54677] - Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH. The observed molecular weight as seen on this gel as greater than 250 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NBP2-54677-img0006.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human pancreas shows low positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0013.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-85728-img0015.jpg)
![Western Blot: Ku70/XRCC6 Antibody [NBP2-38567] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Western-Blot-NBP2-38567-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Ku70/XRCC6 Antibody [NBP2-38567] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human cerebral cortex, kidney, skin and testis using Anti-XRCC6 antibody NBP2-38567 (A) shows similar protein distribution across tissues to independent antibody NBP2-38954 (B).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody [NBP2-38567] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38567-img0004.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0015.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0012.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0013.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-38567] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-Immunohistochemistry-Paraffin-NBP2-38567-img0014.jpg)