| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-306SS | |||
| Azide and BSA Free | NBP2-80586 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-154SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83077-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-316SS | |||
![Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Western-Blot-NB100-544-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.")
![<b>Biological Strategies Validation. </b>RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0004.jpg](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - L.36 PDAC cells were treated two hours using 5 mM hydroxyurea to induce RPA32 Ser33 phosphorylation. The antibody produces clear nuclear staining of treated cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0002.jpg)
![RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0003.jpg](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0003.jpg)
![Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - ATM [p Ser1981] antibody (10H11.E12) [NB100-306] was tested in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-10H11-E12-Immunohistochemistry-NB100-306-img0005.jpg "Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - ATM [p Ser1981] antibody (10H11.E12) [NB100-306] was tested in mouse spleen using DAB with hematoxylin counterstain.")
![Western Blot: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Analysis of ATM-kinase, using ATM [p Ser1981] antibody (10H11.E12) [NB100-306]. Sample: Irradiated or peroxidated human fibroblasts. Observed molecular weight 370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-10H11-E12-Western-Blot-NB100-306-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Human lymphoblastoid cells stained with ATM [p Ser1981] Antibody (10H11.E12). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-10H11-E12-Immunocytochemistry-Immunofluorescence-NB100-306-img0006.jpg)
![Simple Western: Rad50 Antibody [NB100-154] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad50-Antibody-Simple-Western-NB100-154-img0004.jpg "Simple Western: Rad50 Antibody [NB100-154] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Rad50 Antibody [NB100-154] - WB detection of RAD50 protein in 30ug whole cell lysate of 293T cells using Rad50 antibody at 1:2000 dilution (Submitted via Verified Customer Review).](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NB100-154-img0005.jpg)
![Western Blot: Rad50 Antibody [NB100-154] - Detection of Rad50 in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NB100-154-img0002.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0012.jpg "Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left panel) or with vehicle (right control: 0.1% DMSO in media).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 uM) and iron (200 ug/ml) and stained with anti-Phospho-H2AX antibody.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Western blot analysis of Jurkat cells (Lane 1) and Jurkat cells treated with staurosporine (Lane 2) blotted with Histone H2AX [p Ser139] antibody (3F2). Bands indicate an observed molecular weight of ~16 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Western-Blot-NB100-74435-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - STED image of MDA-MB-231 cells after 2 Gy irradiation. Blue channel: DAPI, window level [0 255]. Green channel: secondary antibody Alexa Fluor 488, window level [140 255]. Scale bar: 20 um. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Immunofluorescence analysis using Histone H2AX [p Ser139] antibody (3F2).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - IF image of phosphorylated H2AX in gamma irradiated Hela cells.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0015.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody (3F2) was used at 10 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Evidence of DNA damage & oxidative stress in islets from HFD treated mice. (a) Representative immunofluorescent staining of gamma H2AX, insulin, & DAPI for islets from each treatment group. (b) Representative immunofluorescent staining of 4-hydroxynonenal (4-HNE), insulin, & DAPI for islets from each treatment group. (c) Representative immunofluorescent staining of nitrotyrosine, insulin, & DAPI for islets from each treatment group. (d–g) qRT-PCR analysis of total islet RNA for antioxidant genes, including Gpx1, SOD1, Nrf2, & Ppargc1 alpha . (n = 3) *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-03869-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74435_mouse-monoclonal-histone-h2ax-p-ser139-antibody-3f2-31020241534396.jpg)
![Western Blot: ATM Antibody (5C2) [NB100-220] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (5C2) [NB100-220] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Observed molecular weight ~320 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-5C2-Western-Blot-NB100-220-img0007.jpg "Western Blot: ATM Antibody (5C2) [NB100-220] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (5C2) [NB100-220] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Observed molecular weight ~320 kDa. Theoretical molecular weight 351 kDa.")
![Immunocytochemistry/Immunofluorescence: ATM Antibody (5C2) [NB100-220] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM protein stained by ATM antibody (5C2) [NB100-220] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ATM-Antibody-5C2-Immunocytochemistry-Immunofluorescence-NB100-220-img0004.jpg)
![Western Blot: ATM Antibody (5C2) [NB100-220] - Absence of Atm does not relieve the selective pressure to inactivate p53 during chemical carcinogenesis.A. Representative images of 3MC-fibrosarcomas immunostained for Arf, p53 & p21 (see also Table 1). The upper & middle rows are representative of the large majority of fibrosarcomas developed in wild-type (upper) & Atm-null (middle) mice, which are consistent with a mutant p53 (i.e. strongly positive for p53 & negative for p21). The lower row is representative of the fibrosarcomas developed in Arf-null mice, which are consistent with a functional p53 (i.e. very weakly positive for p53 & positive for p21). B. Examples of cancer cell lines established from 3MC-fibrosarcomas (each line derives from an independent fibrosarcoma). The genotype of the mice where the 3MC-fibrosarcomas were generated is indicated, as well as, the status of p53 as determined by a nutlin-sensitivity assay (see Supplementary Figures S5 & S6). Cell lines were exposed to 10 Gy & protein extracts were obtained 1h & 24h after irradiation. The levels of the indicated proteins were determined by immunoblotting using beta -actin as loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19421407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-220_mouse-monoclonal-atm-antibody-5c2-31020241612655.jpg)
![Immunohistochemistry: ATM Antibody (5C2) [NB100-220] - Limited role of Atm in p53-mediated tumor suppression & DNA damage response in chemically-induced fibrosarcomas.A. Mice of the indicated genotypes, wt (n = 15), p53-super (n = 16), Atm-null (n = 9), p53-super/Atm-null (n = 13) & Arf-null (n = 12), were injected intramuscularly with 3-methyl-cholanthrene (3MC) & tumour development was monitored. Kaplan-Meier tumour-free curves were obtained & statistical significant differences (logrank test) were found for wt vs. p53-super (p<0.005), Atm-null vs. p53-super/Atm-null (p<0.001) & Arf-null vs. wt (p<0.0001). No significant differences were found for wt vs. Atm-null (p = 0.11), or p53-super vs. p53-super/Atm-null (p = 0.18). B. Quantification of the persistent DNA damage response ( gamma H2AX), proliferation (Ki67), & apoptosis (activated caspase-3) in 3MC-fibrosarcomas generated in mice of the indicated genotypes. Quantifications are relative to the 3MC-fibrosarcomas in wild-type mice. Values correspond to the average & standard deviation (n = 5 per genotype). Examples of the immunostainings are shown in the panels at the right. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19421407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-220_mouse-monoclonal-atm-antibody-5c2-3102024165717.jpg)
![Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - BAG3P209L aggregation leads to co-aggregation of proteasomal substrates. a Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Scale bar = 5 μm. b Suppression of GFP-HttQ74 aggregation of cells expressing a control, FLAG-BAG3WT or FLAG-BAG3P209L. Western blot against indicated antibodies is shown. c Quantification of GFP-HttQ74 aggregation of experiments similar to b. Relative percentage of SDS-insoluble protein levels are shown. Data represents the mean & standard deviation of three independent experiments. d Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Left column BAG3, middle column ubiquitin & right column is the merge of BAG3 (green), ubiquitin (red), & DAPI (blue). Scale bar = 5 μm. e Fractionation of HEK293 cells expressing HSPB8, a control or BAG3WT or BAG3P209L, together with either Ub-R-GFP or GFP-ODC (ornithine decarboxylase). Western blot against GFP, FLAG (BAG3), Myc (HSPB8), & tubulin are shown. f Fractionation of HEK293 cells expressing HSPB8 & indicated BAG3 variants. Western blot using ubiquitin (FK2) & tubulin antibodies are shown. The same samples as in Fig. 5e have been used, loading control is therefore the same. Source data are provided as a Source data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30559338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-220_mouse-monoclonal-atm-antibody-5c2-31020241553206.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Human primary cells stained with Mre11 antibody at a dilution of 1:60. cells were fixed in 1:1 Acetone/Methanol. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Immunohistochemistry-NB100-473-img0008.jpg "Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Human primary cells stained with Mre11 antibody at a dilution of 1:60. cells were fixed in 1:1 Acetone/Methanol. ICC/IF image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Mre11 Antibody (12D7) [NB100-473] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. A band appears at the theoretical molecular weight of 80 kDa.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Western-Blot-NB100-473-img0010.jpg)
![Western Blot: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of human Mre11-transfected 293T cells. 7.5% SDS-PAGE. Mre11 antibody [12D7] (NB100-473). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Bands are prominent at the theoretical molecular weight of 80 kDa.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Western-Blot-NB100-473-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Analysis of Mre11 antibody (12D7) on lymphoblastoid cells. Dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Immunocytochemistry-Immunofluorescence-NB100-473-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (NB100-473).Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Immunocytochemistry-Immunofluorescence-NB100-473-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mouse fibroblasts stained with Mre11 Antibody (12D7). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Mre11-Antibody-12D7-Immunocytochemistry-Immunofluorescence-NB100-473-img0020.jpg)
![Western Blot: Mre11 Antibody (12D7) [NB100-473] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (NB100-473) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-473_mouse-monoclonal-mre11-antibody-12d7-91020242091437.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining in human cerebral cortex and liver tissues . Corresponding ATRX RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0026.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining in human cerebral cortex and liver tissues . Corresponding ATRX RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/ATRX-Antibody-Western-Blot-NBP1-83077-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP1-83077] - Staining of human cell line A549 shows localization to nucleoplasm and nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83077-img0029.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0027.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0022.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0023.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human uterine cervix shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0025.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-Antibody-Immunohistochemistry-Paraffin-NB100-204-img0008.jpg "Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-Antibody-Immunohistochemistry-Paraffin-NB100-204-img0007.jpg)
![Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SMC1-Antibody-Simple-Western-NB100-204-img0006.jpg)
![SMC1 Antibody [NB100-204] - Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC1 antibody NB100-204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Previous](http://images.novusbio.com/fullsize/SMC1-Antibody-NB100-204-img0009.jpg)
![SMC1 Antibody [NB100-204] - Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody NB100-204 used for IP at 6 ug per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody NB2-76412. For blotting immunoprecipitated SMC1, NB100-204 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.](http://images.novusbio.com/fullsize/SMC1-Antibody-NB100-204-img0010.jpg)
![Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Simple-Western-NB100-316-img0005.jpg "Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0004.jpg)
![Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (1D7) [NB100-221] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-1D7-Western-Blot-NB100-221-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (1D7) [NB100-221] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Sample (30 ug of whole cell lysate). A: HepG2. 7.5% SDS PAGE. Nbs1 Antibody (1D7) [NB100-221] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Observed Molecular Weight at ~97 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-1D7-Western-Blot-NB100-221-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Nbs1 Antibody (1D7) [NB100-221] - Nbs1 antibody [1D7] detects Nbs1 protein at nucleus by immunofluorescent analysis.</br>Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Nbs1 stained by Nbs1 antibody [1D7] (NB100-221) diluted at 1:500.</br>Red: phalloidin, a cytoskeleton marker, diluted at 1:200.](http://images.novusbio.com/fullsize/nb100-221_mouse-monoclonal-nbs1-antibody-1d7-910202420104417.jpg)
![Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nbs1 antibody [1D7] (NB100-221) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-221_mouse-monoclonal-nbs1-antibody-1d7-western-blot-1411202518425223.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0007.jpg "Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Knockout-Validated-NB100-926-img0020.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.")
![<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0011.jpg)
![Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical analysis of human breast tumors stained with 53BP1 antibody (Catalog #NB100-304). Image from verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-NB100-926-img0021.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0023.jpg)
![Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using 53BP1 Antibody (Catalog #NB100-304).](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0009.jpg)
![Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunohistochemistry-NB100-926-img0012.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53BP1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml 53BP1 Antibody (Catalog #NB100-304) in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0010.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0022.jpg)