| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35698-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03454-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42205-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22196-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19255-0.2mg | |||
![Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-Paraffin-NB100-1534-img0002.jpg "Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-NB100-1534-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - Fluorescence micrographs showing EGFR (Alexa 488; green), Rictor (Alexa 488; yellow) & cell nuclei (Hoechst 33342; blue) in GBM4 GBM-derived cancer stem-like cell line, & Gli36, U251MG, U118MG & LN229 GBM cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415392527.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - The combined silencing of Rictor & EGFR in vivo results in a complete inhibition of tumor growth.U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice (n = 6−8). Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline & 5% sucrose in drinking water. a) On day 49, animals were imaged by Maestro™ fluorescence imaging unit for the expression of tRFP co-expressed with the shRNA sequences upon doxycycline-induced expression. Mice were then terminated & brains were harvested, sectioned & stained for nuclei, Rictor, EGFR & p(473)-AKT & imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. b) A representative brain section from U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor tumor groups is shown: tRFP (red) & Hoechst (blue). c) A representative tumor section from U251Ng2x, U251Rictor & U251EGFR tumor groups is shown: nuclei (blue), rRFP (red), Rictor (yellow), EGFR (green) & p(473)-AKT (orange). d) The expression of EGFR (left axis), Rictor (right axis) & p(473)-AKT (right axis) in U251Ng2x, U251Rictor, U251EGFR tumor sections were quantified (positive staining normalized to Hoechst nuclei staining). e) Tumor sizes were estimated by quantification of tumor areas in brain sections from all groups (left axis). The expression of the proliferation marker Ki67 in the tumor (proliferating fraction) was also quantified (right axis). *p-value ≤0.05; **p-value ≤0.01; ***p-value ≤0.001 compared to control untreated cells. ‡: No tumor was detected in the U251EGFR/Rictor group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415525248.jpg)
![Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NB100-844-img0002.jpg "Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - )Staining of paraffin embedded Human Thyroid. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-844-img0004.jpg)
![Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NB100-844-img0001.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - Staining of paraffin embedded Human Kidney. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-844-img0003.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Brain Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0009.jpg "Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Brain Antibody Dilution: 1.0 ug/ml")
![Western Blot: CLCN5 Antibody [NBP1-69123] - Mouse Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0002.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Liver. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0003.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Sample Type: Mouse Brain Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0004.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Sample Type: Mouse Heart Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0005.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Sample Type: Mouse Kidney Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0006.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0007.jpg)
![Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Muscle Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CLCN5-Antibody-Western-Blot-NBP1-69123-img0008.jpg)
![Western Blot: DNA Ligase IV Antibody [NB110-57379] - HepG2 cell lysate.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NB110-57379-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NB110-57379] - HepG2 cell lysate.")
![Immunohistochemistry: DNA Ligase IV Antibody [NB110-57379] - Immunohistochemical staining of human pancreatic tissue using NB110-57379 at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-NB110-57379-img0003.jpg)
![Immunohistochemistry: XRCC4 Antibody [NBP3-35698] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using XRCC4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-1012202411545584.jpg "Immunohistochemistry: XRCC4 Antibody [NBP3-35698] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using XRCC4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: XRCC4 Antibody [NBP3-35698] - Immunofluorescence analysis of 293T cells using XRCC4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-101220241155592.jpg)
![Immunohistochemistry: XRCC4 Antibody [NBP3-35698] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using XRCC4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-101220241214412.jpg)
![Immunohistochemistry: XRCC4 Antibody [NBP3-35698] - Immunohistochemistry analysis of paraffin-embedded Human appendix using XRCC4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-1012202411592897.jpg)
![Western Blot: XRCC4 Antibody [NBP3-35698] - Western blot analysis of various lysates using XRCC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-101220241214438.jpg)
![Western Blot: XRCC4 Antibody [NBP3-35698] - Western blot analysis of lysates from Rat testis, using XRCC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 6s.](http://images.novusbio.com/fullsize/nbp3-35698_rabbit-xrcc4-pab-1012202411592867.jpg)
![Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Analysis of extracts of various cell lines, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Western-Blot-NBP3-03454-img0004.jpg "Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Analysis of extracts of various cell lines, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP3-03454] - Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (NBP3-03454). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03454-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Mouse kidney using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-03454-img0002.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Human stomach using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-03454-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Knockout-Validated-NBP3-03454-img0005.jpg)
![Western Blot: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Western-Blot-NBP2-37583-img0002.jpg "Western Blot: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Immunocytochemistry-Immunofluorescence-NBP2-37583-img0003.jpg)
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Immunohistochemistry-NBP2-37583-img0005.jpg)
![Flow Cytometry: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Flow-Cytometry-NBP2-37583-img0004.jpg)
![ELISA: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-ELISA-NBP2-37583-img0001.jpg)
![Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Western-Blot-NBP2-42205-img0004.jpg "Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0003.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0006.jpg "Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0003.jpg)
![Flow Cytometry: PLK1 Antibody (3C11) [NBP2-22196] - Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Flow-Cytometry-NBP2-22196-img0001.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0005.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0002.jpg)
![ELISA: PLK1 Antibody (3C11) [NBP2-22196] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-ELISA-NBP2-22196-img0004.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Analysis of Histone H2AX [p Ser139] antibody (9F3) Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate. Bands appear at an observed molecular weight of ~15 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-9F3-Western-Blot-NBP1-19255-img0001.jpg "Western Blot: Histone H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Analysis of Histone H2AX [p Ser139] antibody (9F3) Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate. Bands appear at an observed molecular weight of ~15 kDa.")
![Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0082.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0002.jpg)
![Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-233-img0054.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")