| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2090SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88517-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70908 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76638SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2064-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB100-185SS | |||
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NB21-2090-img0002.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.")
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NB21-2090-img0001.jpg)
![Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Simple-Western-NB21-2090-img0003.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0011.jpg "Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunohistochemistry-Paraffin-H00253260-M01-img0008.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0012.jpg)
![Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunoprecipitation-H00253260-M01-img0009.jpg)
![Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Sandwich-ELISA-H00253260-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RBM14 Antibody [NBP1-84416] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84416-img0009.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0013.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0011.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0012.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88517-img0008.jpg "Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0014.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0001.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0011.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0012.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0013.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0021.jpg "Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0001.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0002.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0003.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human kidney. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0004.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0005.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0006.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0007.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0008.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0009.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0010.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human pancreas. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0011.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0012.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0013.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0014.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0015.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0016.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0017.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0018.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0019.jpg)
![Immunohistochemistry: hnRNP L Antibody (OTI2E4) [NBP2-45990] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Immunohistochemistry-NBP2-45990-img0020.jpg)
![Adhesion Activation: hnRNP L Antibody (OTI2E4) [NBP2-45990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPL cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPL.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-OTI2E4-Adhesion-Activation-NBP2-45990-img0022.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-OTI1F12-Immunocytochemistry-Immunofluorescence-NBP2-46125-img0005.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 u/mL.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NBP1-76638-img0001.jpg)
![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - miR-663 controls PIN-1-mediated apoptosis & p53 mitochondrial functiona Immunoblot showing BTG2 downmodulation 72 h after siRNA transfection. TUBULIN was used as protein loading control. Normalized BTG2 levels are expressed as fold decrease relative to cntrl treated cells. b Western blotting showing BTG2 modulation in NIH-H460 after the indicated treatments. Double transfection of anti-BTG2 siRNA together with LNA-663 restores BTG2 levels of control cells, counteracting LNA-mediated induction. PARP-1 cleavage is reduced to 60%. TUBULIN was used as protein loading control. Numbers indicate BTG2 levels fold change relative to cntrl treated cells, & cleaved PARP-1 levels fold decrease relative to LNA-663/cntrl siRNA-treated cells. The values were normalized to TUBULIN signal, as measured by densitometric analyses. c Subcellular BTG2 & PIN-1 localization in NIH-H460 cells treated with control LNA or LNA-663 was assessed by immunofluorescence staining. d Mitochondrial membrane potential of NIH-H460 cells upon miR-663 downmodulation was measured by JC-1 staining. An increase of the ratio between green & red signals is indicative of membrane depolarization typical of apoptotic cells. e Western blotting showing PIN-1 modulation in NIH-H460 subcellular fractions 24 h after LNA-663 treatment. Cytoplasmic accumulation & nuclear decrease of PIN-1 are expressed as fold changes relative to cntrl-treated cells. The histogram shows the ratio between cytoplasmic & nuclear signal. f Immunoblot of p53 in NIH-H460 subcellular fractions. Hsp90 & Bcl-2 were used as cytoplasmic & mitochondrial markers, respectively. Mitochondrial p53 modulation is expressed as fold increase relative to cntrl treated cells & normalized to Bcl-2 signal Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29352138), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-31020241616377.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunofluorescence of p53 DINP1 in human liver tissue with p53 DINP1 antibody at 5 ug/ml. <br><br>Green: p53 DINP1 antibody <br> Red: Phylloidin staining<br> Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202419353887.gif)
![Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunohistochemical staining of mouse liver using p53 DINP1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202420352418.jpg)
![Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Western blot analysis of extracts of Mouse thymus, using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Western-Blot-NBP3-15450-img0006.jpg "Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Western blot analysis of extracts of Mouse thymus, using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Human thyroid cancer using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Immunohistochemistry-Paraffin-NBP3-15450-img0001.jpg)
![Immunoprecipitation: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Topoisomerase I antibody (NBP3-15450). Western blot was performed from the immunoprecipitate using Topoisomerase I antibody (NBP3-15450) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Immunoprecipitation-NBP3-15450-img0007.jpg)
![Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Analysis of various lysates using DNA topoisomerase I (DNA topoisomerase I (TOP1)) Rabbit mAb at 1：1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15450_rabbit-topoisomerase-i-mab-3d4w6-western-blot-15102024111447..jpg)
![Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15450_rabbit-topoisomerase-i-mab-3d4w6-immunocytochemistry-immunofluorescence-1510202411164..jpg)
![Immunocytochemistry/ Immunofluorescence: Topoisomerase I Antibody (3D4W6) [Topoisomerase I] - Confocal imaging of U-2 OS cells using Topoisomerase I Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15450_rabbit-topoisomerase-i-mab-3d4w6-52202517202937.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0002.jpg "Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0001.jpg)
![Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Western-Blot-NBP2-67326-img0008.jpg "Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BS](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0009.jpg)
![Flow Cytometry: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Flow-Cytometry-NBP2-67326-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0006.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0007.jpg)
![Western blot analysis on different lysates with NBP2-67326 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HDLM-2 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67326 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67326_-western-blot-639204586668668946.jpg)
![Western blot analysis on different lysates with NBP2-67326 at 1/1,000 dilution. Lane 1: Rat stomach tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67326 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67326_-western-blot-639204597489977519.jpg)