| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26297SS | |||
| Azide and BSA Free | NBP2-80867 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54808SS | |||
| Azide and BSA Free | NBP2-80729 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-316SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-598SS | |||
| Azide and BSA Free | NBP2-80600 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-600SS | |||
| Azide and BSA Free | NBP2-80602 | |||
![Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Knockout-Validated-NBP2-26297-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunocytochemistry-Immunofluorescence-NBP2-26297-img0008.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0011.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red).](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Flow-Cytometry-NBP2-26297-img0009.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0010.jpg)
![ELISA: Nbs1 Antibody (7E4A2) [NBP2-26297] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297].](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-ELISA-NBP2-26297-img0014.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Simple-Western-NBP2-26297-img0017.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Knockout-Validated-NBP2-54808-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Simple-Western-NBP2-54808-img0002.jpg)
![Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Immunohistochemistry-NBP2-54808-img0004.jpg)
![Flow (Intracellular): FANCD2 Antibody (1290D) [NBP2-54808] - An intracellular stain was performed on HeLa Cells with FANCD2 Antibody (1290D) (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Flow-Intracellular-NBP2-54808-img0005.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Western-Blot-NBP2-54808-img0006.jpg)
![Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg)
![Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/p53-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB200-171-img0073.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0003.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0007.jpg "Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0005.jpg)
![Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Simple-Western-NB100-316-img0005.jpg "Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0003.jpg)
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0004.jpg)
![Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/H2AX-Antibody-Western-Blot-NBP2-27428-img0004.jpg "Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0007.jpg)
![Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27428] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunohistochemistry-Paraffin-NBP2-27428-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0006.jpg)
![Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Western-Blot-NB100-598-img0006.jpg "Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [NB100-598] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Immunocytochemistry-Immunofluorescence-NB100-598-img0005.jpg)
![Flow Cytometry: BRCA1 Antibody (RAY) [NB100-598] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Flow-Cytometry-NB100-598-img0008.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0004.jpg "Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.")
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0006.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0003.jpg)
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0005.jpg)
![Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Western-Blot-NB100-206-img0006.jpg "Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.")
![Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-206-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0007.jpg)
![SMC1 [p Ser966] Antibody [NB100-206] - Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody NB100-206 used for IP at 3 ul per reaction. Phospho SMC1 (S966) was also immunoprecipitated by rabbit anti-SMC1 antibody NB100-204. For blotting immunoprecipitated Phospho SMC1 NB100-206 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody NB100-204 was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-NB100-206-img0009.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0003.jpg "Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunocytochemistry-Immunofluorescence-NB100-404-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade diluted at 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - NB100-404 was used at 1:1000 dilution for Western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody (13B3) (NB100-147) was used as a loading control.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0004.jpg)
![Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404] - 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunoprecipitation-NB100-404-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - DAG-induced DNA damage is repaired by homologous recombination. A549 cells were transfected with either negative control (C) or three BRCA1-targeting siRNAs (B1, siBRCA1-2; B2, siBRCA1-15; or B3, siBRCA1-17) for 24 h. Cells were then seeded in 96-well culture plates and treated with different concentrations of VAL-083 (0, 100 nM, 500 nM, 1 uM, 1.5 uM, 2.5 uM, 5 uM, 10 uM, 25 uM, 50 uM, 100 uM, and 200 uM) for 5 days. Following the treatment, crystal violet assay was performed to detect the absorbance at 560 nm wavelength. Cell lysates from the control or BRCA1-knockdown cells were analyzed by western blot with antibody against BRCA1, and GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1069-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockdown-Validated-NB100-404-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody, diluted at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockout-Validated-NB100-404-img0015.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (NB100-404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nb100-404_mouse-monoclonal-brca1-antibody-6b4-91020242091412.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0034.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)