| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76639-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73472 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06609SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22128SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
![Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), NBP1-76639 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242016915.gif)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242045643.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Carcinoma. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420243374.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Tissue. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PUMA antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420285891.gif)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0028.jpg "Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody [NBP1-32851] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term.](http://images.novusbio.com/fullsize/ATRX-Antibody-Western-Blot-NBP1-32851-img0020.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human endometrial carcinoma. Rad54 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0011.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human breast carcinoma. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0025.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0030.jpg)
![Immunoprecipitation: ATRX Antibody [NBP1-32851] - Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 ug of ATRX antibody [C3], C-term (NBP1-32851). Western blot analysis was performed using ATRX antibody [C3], C-term (NBP1-32851). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunoprecipitation-NBP1-32851-img0016.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202491732..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202492044..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202492449..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - mRNA expression & protein complex formation of various ChRFs in the brain & in embryonic stem cells (ESCs). (A) Schematic of regions that were assessed for RNA levels of several ChRFs: Bs, brain stem; Ce, cerebellum; Mb, midbrain; Hth, hypothalamus; Hc, Th, S, hippocampus/thalamus/septum; Ctx, cortex; & Ob, olfactory bulb. (B) ChRFs fall into two large expression groups (I + II) in the brain. RT-qPCR results were expressed as delta CT values (reference gene: Gapdh) which were then centered at the median & subjected to hierarchical clustering. Red color indicates high expression (negative delta CT value) & green color indicates low expression (positive delta CT value). (C) Superose 6 size exclusion chromatography of ChRF peak fractions after anion exchange chromatography followed by immunoblot analysis of CHD3, CHD5, & ATRX as well as HDAC1, 2, & 3. The arrowhead indicates the void volume of the column, the asterisk marks a scanning artifact. Molecular masses of defined marker proteins are indicated at the bottom of their corresponding elution fractions. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnbeh.2015.00313/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-31020241616093.jpg)
![Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP1-32851] - ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATRX stained by ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-910202419593566.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-91020242021954.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-91020242011440.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0016.jpg "Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of")
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0018.jpg)
![Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody , and then analyzed by flow cytometry (1:100).](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Flow-Cytometry-NBP1-48291-img0017.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0001.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0003.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0004.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0009.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0011.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0014.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80867-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80867-img0003.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80867-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80867-img0002.jpg)
![ELISA: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-ELISA-NBP2-80867-img0005.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Simple-Western-NBP2-80867-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Nbs1 Antibody (7E4A2) - Azide and BSA Free [NBP2-80867] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Azide-and-BSA-Free-Knockout-Validated-NBP2-80867-img0006.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
![Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NBP1-06609-img0005.jpg)
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg)
![Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-90103-img0007.jpg "Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90103-img0009.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0014.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0011.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0012.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0013.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0006.jpg "Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.")
![Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-22128-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0005.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0002.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0004.jpg)
![ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-ELISA-NBP2-22128-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31294 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. HDAC1 antibody (NBP1-31294) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0018.jpg "<b>Biological Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0019.jpg)
![Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:600 and with DDDDK tag antibody (NBP2-43574) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0007.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NBP2-15791-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15791-img0022.jpg)
![Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0021.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0020.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-91020242075966.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-9102024209142.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-1610202419405783.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)