| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB90782-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89524-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06705SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
![Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/B7-H1-PD-L1-CD274-Antibody-2096A-Immunohistochemistry-MAB90782-img0002.jpg "Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89524-img0007.jpg "Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0014.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Tonsil shows moderate nuclear positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0015.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Western-Blot-NBP2-02004-img0014.jpg "Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-02004-img0013.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human tonsil using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0012.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATRIP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0003.jpg)
![Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Western-Blot-NBP2-02004-img0004.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0005.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0006.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human breast tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0007.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human Kidney tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0008.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human liver tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0009.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human pancreas tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0010.jpg)
![Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human prostate tissue using anti-ATRIP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-02004-img0011.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Hela cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0001.jpg)
![Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Jurkat cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATRIP-Antibody-5E7-Flow-Cytometry-NBP2-02004-img0002.jpg)
![Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Western-Blot-NBP3-14678-img0002.jpg "Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.")
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human HeLa cells treated with etoposide. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunocytochemistry-Immunofluorescence-NBP3-14678-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human breast carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:100. Secondary: DyLight(R) 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0007.jpg)
![Flow Cytometry: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Rabbit Histone H2AX antibody (BLR053F) or isotype control (unshaded). Secondary: DyLight(R) 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Flow-Cytometry-NBP3-14678-img0008.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human basal cell carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of mouse CT26 tumor. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0005.jpg)
![Immunoprecipitation: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-gamma-H2AX recombinant monoclonal antibody used for IP at 20 ul per reaction. gamma-H2AX was also immunoprecipitated by rabbit anti-gamma-H2AX antibody. For blotting immunoprecipitated gamma-H2AX, was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower panel: Rabbit anti-H2AX](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-Immunoprecipitation-NBP3-14678-img0001.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Western-Blot-NBP3-15378-img0004.jpg "Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Mouse brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Rat brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2AX Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517192975.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223915.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251718450.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517184545.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251720298.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517223963.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517273511.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517284912.jpg)
![Immunohistochemistry: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Histone H2AX Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-5220251717282.jpg)
![Western Blot: Histone H2AX Antibody (4Z3S8) [Histone H2AX] - Western blot analysis of various lysates using Histone H2AX Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15378_rabbit-histone-h2ax-mab-4z3s8-52202517152711.jpg)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0012.jpg "Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunocytochemistry-Immunofluorescence-H00005347-M01-img0007.jpg)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0010.jpg)
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0011.jpg)
![Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Proximity-Ligation-Assay-H00005347-M01-img0005.jpg)
![Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunoprecipitation-H00005347-M01-img0008.jpg)
![ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-ELISA-H00005347-M01-img0013.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0014.jpg "Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunocytochemistry-Immunofluorescence-H00003065-M06-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Immunohistochemistry-Paraffin-H00003065-M06-img0009.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0010.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0011.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0012.jpg)
![Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-Western-Blot-H00003065-M06-img0013.jpg)
![ELISA: HDAC1 Antibody (1D6) [H00003065-M06] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-1D6-ELISA-H00003065-M06-img0007.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0011.jpg "Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunocytochemistry-Immunofluorescence-H00011200-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunohistochemistry-Paraffin-H00011200-M01-img0009.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0012.jpg)
![Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Sandwich-ELISA-H00011200-M01-img0010.jpg)
![Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Western-Blot-NBP2-88933-img0008.jpg "Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunocytochemistry-Immunofluorescence-NBP2-88933-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human human embryonal carcinoma of testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis cancer, seminoma shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0006.jpg)
![Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06705-img0004.jpg "Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06705] - Staining of paraffin-embedded mouse skin with Nbs1 Antibody [NBP1-06705].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06705-img0003.jpg)
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0002.jpg "Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0004.jpg)
![Flow Cytometry: PD-L1 Antibody [NB300-903] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Flow-Cytometry-NB300-903-img0005.jpg)
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed AA431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0003.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)