| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44978 | |||
| Azide and BSA Free | NBP2-34605-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43870 | |||
| BSA Free | NBP2-52938-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22128SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06609SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB90782-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
![Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Western-Blot-NBP2-34308-img0004.jpg "Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).")
![Immunohistochemistry-Paraffin: p53 Antibody (DO-7) [NBP2-34308] - Formalin-fixed, paraffin-embedded human colon stained with p53 MAb (DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Immunohistochemistry-Paraffin-NBP2-34308-img0001.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0002.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0003.jpg)
![Western Blot: ATRX Antibody (CL0537) [NBP2-52938] - analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Western-Blot-NBP2-52938-img0023.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody (CL0537) [NBP2-52938] - analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in U251 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0022.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in A431 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in HeLa cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in MCF7 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0003.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0016.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human glioma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0017.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0018.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0006.jpg "Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.")
![Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-22128-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0005.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0002.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0004.jpg)
![ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-ELISA-NBP2-22128-img0001.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0019.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:600 and with DDDDK tag antibody (NBP2-43574) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0007.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NBP2-15791-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15791-img0022.jpg)
![Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0021.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. <br></br>Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0020.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-91020242075966.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-1610202419405783.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05447-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0005.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0003.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Mouse testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0004.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385445.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of NIH/3T3, using Phospho-ATR-S428 antibody at 1:400 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385627.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31294 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. HDAC1 antibody (NBP1-31294) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/B7-H1-PD-L1-CD274-Antibody-2096A-Immunohistochemistry-MAB90782-img0002.jpg "Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.")
![Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0003.jpg "Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.")
![Western Blot: ATM Antibody [NB100-678] - MDA-MB-231 and MCF-7 whole cell lysates were loaded with 50 ug/lane. 8% SDS-PAGE. ATM Antibody (NB100-678) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0009.jpg)
![Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ATM-Antibody-Immunohistochemistry-NB100-678-img0007.jpg)
![Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0008.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
