| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34401-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05210-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92714SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44939 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90042-25ul | |||
![Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Western-Blot-NBP2-34353-img0003.jpg "Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM248) [NBP2-34353] - Formalin-fixed, paraffin-embedded human schwannoma stained with GFAP monoclonal antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Immunohistochemistry-Paraffin-NBP2-34353-img0001.jpg)
![Flow Cytometry: GFAP Antibody (SPM248) [NBP2-34353] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (SPM248) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Flow-Cytometry-NBP2-34353-img0002.jpg)
![Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Western-Blot-NBP1-05210-img0006.jpg "Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunocytochemistry-Immunofluorescence-NBP1-05210-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunohistochemistry-Free-Floating-NBP1-05210-img0005.jpg)
![Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05210_mouse-monoclonal-nf-h-antibody-9b12-immunocytochemistry-immunofluorescence-132202617595724.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-NBP1-92714-img0002.jpg "Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-92714] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to GAP43, NBP1-92714, dilution 1:2,000 in green, and costained with rabbit pAb to alpha-II spectrin, dilution 1:1,000 in red. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunofluorescence-NBP1-92714-img0001.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3. Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0006.jpg "Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3. Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Bovine ovarian granulosa cells. Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blue). Control was obtained by omitting the primary antibody. Fixation: 4% PF for 7 minutes at rt. Permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt. Primary antibody: 1:50 or 1:100 in 1% BSA/PBS over night at 4C. Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500. DNA Counterstaining: mounting medium with DAPI. Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope. Image from verified customer review.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00004627-M03-img0007.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of monoclonal antibody to MYH9 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 0.5 ug/ml](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunohistochemistry-Paraffin-H00004627-M03-img0003.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in HeLa.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0004.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in human kidney.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of monoclonal antibody to MYH9 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00004627-M03-img0002.jpg)
![ELISA: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Detection limit for recombinant GST tagged MYH9 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-ELISA-H00004627-M03-img0001.jpg)
![Western Blot: ERManI Antibody (3C2) - Azide and BSA Free [NBP2-80726] - Western blot analysis of ERManI expression in 1) NTERA-2 and 2) A-431 whole cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ERManI-Antibody-3C2-Azide-and-BSA-Free-Western-Blot-NBP2-80726-img0001.jpg "Western Blot: ERManI Antibody (3C2) - Azide and BSA Free [NBP2-80726] - Western blot analysis of ERManI expression in 1) NTERA-2 and 2) A-431 whole cell lysates. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: ERManI Antibody (3C2) - Azide and BSA Free [NBP2-80726] - ERManI antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ERManI-Antibody-3C2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80726-img0002.jpg)
![Immunohistochemistry: ERManI Antibody (3C2) - Azide and BSA Free [NBP2-80726] - IHC staining of ERManI in human breast cancer using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ERManI-Antibody-3C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80726-img0003.jpg)
![Western Blot: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Western-Blot-NBP2-34654-img0006.jpg "Western Blot: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34654-img0005.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 8 Antibody (K8.8) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34654-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Staining of human colon carcinoma.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34654-img0001.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 1 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Simple-Western-NBP2-34654-img0003.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.1 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Simple-Western-NBP2-34654-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/OCT6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13349-img0003.jpg "Immunocytochemistry/Immunofluorescence: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/OCT6-Antibody-Immunohistochemistry-Paraffin-NBP3-13349-img0005.jpg)
![Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/OCT6-Antibody-Immunohistochemistry-Paraffin-NBP3-13349-img0002.jpg)
![OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: 44840 stained by OCT6 antibody (NBP3-13349) diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13349_rabbit-oct6-pab-immunocytochemistry-immunofluorescence-26112025199115.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Plakophilin 1 Antibody [NBP1-90042] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/Plakophilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90042-img0005.jpg "Immunocytochemistry/Immunofluorescence: Plakophilin 1 Antibody [NBP1-90042] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.")
![Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Plakophilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90042-img0007.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Plakophilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90042-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis using Anti-PKP1 antibody NBP1-90042 (A) shows similar pattern to independent antibody NBP1-90041 (B).](http://images.novusbio.com/fullsize/Plakophilin-1-Antibody-Western-Blot-NBP1-90042-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell lines PC-3 and A-549 using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Plakophilin-1-Antibody-Western-Blot-NBP1-90042-img0009.jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025163536..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-294202516917..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025161213..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2802] diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2942025161556..jpg)
![Immunohistochemistry: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Tau stained by Tau antibody [HL2802] diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-paraffin-2942025161950..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025162224..jpg)
![Tau antibody [HL2802] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2802] (NBP3-45161) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-immunohistochemistry-frozen-141120251855420.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202374820.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GFAP Antibody [NBP2-33774] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NBP2-33774] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33774-img0017.jpg)
![Western Blot: GFAP Antibody [NBP2-33774] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human hippocampus shows positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0039.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0040.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Staining of human cervix, uterine shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202375644..jpg)
