| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48226 | |||
| Azide and BSA Free | NBP2-34705-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48051 | |||
| Azide and BSA Free | NBP3-11500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54594-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44939 | |||
| Azide and BSA Free | NBP2-34654-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-330SS | |||
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-NB100-40844-img0005.jpg "Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and NB100-40843.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunoprecipitation-NB100-40844-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-498-Western-Blot-NBP2-32893-img0001.jpg "Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.")
![Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NB500-177-img0011.jpg "Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NB500-177-img0013.jpg)
![Chromatin Immunoprecipitation: CTCF Antibody [NB500-177] - Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20 micrograms of rabbit anti-CTCF antibody NB500-177. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NB500-177-img0004.jpg)
![Western Blot: CTCF Antibody [NB500-177] - Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NB500-177-img0010.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NB500-177-img0012.jpg)
![Immunoprecipitation: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 (lot NB500-177-6) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (lot NB500-177-5). For blotting immunoprecipitated CTCF, NB500-177 was used at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunoprecipitation-NB500-177-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Immunohistochemistry-Paraffin-NBP2-44690-img0001.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Antibody (SPM249).followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Flow-Cytometry-NBP2-44690-img0002.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Classical swine fever virus (CSFV) infection induces the activation of UPR. (A) RNA extracted from CSFV-infected cells was quantified for the expression of UPR genes Xbp1(s), GRP78, GRP94, EDEM-1, ATF4, ATF6, CHOP, Calreticulin, Calnexin, & ERp57 using q-PCR. Mock-infected PK-15 & Thapsigargin (TG)-treated PK-15 were used as negative & positive controls, respectively, & the fold induction was calculated compared to mock cells at the same time point. Error bars represent the mean ± SD of 3 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. (B,D,E) Immunoblotting analysis of components of UPR signaling pathways during a time course of CSFV infection. Mock or CSFV-infected PK-15 cells lysates were collected at the indicated time points. Lysates were analyzed for the activation of the IRE1 (B), PERK (D) & ATF6 (E) pathway by immunoblotting analysis. Tubulin was used as a loading control, & infection was confirmed by detecting the viral protein Npro. Results of a representative experiment of 2 independent experiments are shown. (C) RNA was collected as described above, & the splicing levels of XBP1 were analyzed with semi-quantitative PCR as described in materials & methods. The length of Xbp1(u) is 474 bp & Xbp1(s) is 448 bp. (F) The relative expression ratios of the targeted proteins/genes were analyzed by densitometric scanning. Error bars represent the mean ± SD of 2 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fmicb.2017.02129/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44690_rat-monoclonal-gp96-hsp90b1-grp94-antibody-spm249-31020241612223.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot Analysis of human A549 cell lysate using SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Western-Blot-NBP2-53407-img0009.jpg "Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot Analysis of human A549 cell lysate using SOX2 Antibody (SOX2/1791).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0006.jpg)
![Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Western-Blot-NBP2-53407-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0005.jpg)
![Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1791) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Protein-Array-NBP2-53407-img0007.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP1-31302-img0008.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0010.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0011.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0012.jpg)
![Western Blot: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Western-Blot-NBP2-34269-img0008.jpg "Western Blot: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Immunofluorescence Analysis of HCT116 cells labeling CK8 with Cytokeratin 8 Mouse Monoclonal Antibody (K8.8) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Immunocytochemistry-Immunofluorescence-NBP2-34269-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Immunohistochemistry-Paraffin-NBP2-34269-img0006.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 8 Antibody (K8.8) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Flow-Cytometry-NBP2-34269-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Analysis using the Azide and BSA Free version of NBP2-34269. Staining of human colon carcinoma.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Immunohistochemistry-Paraffin-NBP2-34269-img0002.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.1 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Simple-Western-NBP2-34269-img0003.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) [NBP2-34269] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 1 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Simple-Western-NBP2-34269-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-4D7-Immunocytochemistry-Immunofluorescence-NB100-330-img0003.jpg "Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.")
![Western Blot: Cortactin Antibody [NBP2-15972] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15972] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15972] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody [NBP2-15972] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15972-img0003.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15972] - Paraffin-embedded mouse intestine. Cortactin antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15972-img0005.jpg)
![Western Blot: Cortactin Antibody [NBP2-15972] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0007.jpg)
![Western Blot: Cortactin Antibody [NBP2-15972] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] diluted at 1:10000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15972-img0009.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15972] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15972-img0002.jpg)
![Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Western-Blot-NB300-134-img0004.jpg "Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Immunohistochemistry-NB300-134-img0003.jpg)
![Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-3H11-Western-Blot-NB300-134-img0002.jpg)