| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87426-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85336-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87718-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43820 | |||
| BSA Free | NBP2-46618-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85244-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71086 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52433-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52501-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96425-0.05mg | |||
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining in human cerebral cortex and skin tissues using anti-PFN2 antibody. Corresponding PFN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining in human cerebral cortex and skin tissues using anti-PFN2 antibody. Corresponding PFN2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Profilin 2 Antibody [NBP1-87426] - Analysis in human cell lines A-431 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0009.jpg)
![Western Blot: Profilin 2 Antibody [NBP1-87426] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0006.jpg)
![Western Blot: Profilin 2 Antibody [NBP1-87426] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0007.jpg)
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0010.jpg)
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0011.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85336 (A) shows similar protein distribution across tissues to independent antibody NBP1-85335 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85336 (A) shows similar protein distribution across tissues to independent antibody NBP1-85335 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Villin 1 Antibody [NBP1-85336] - Analysis using Anti-VIL1 antibody NBP1-85336 (A) shows similar pattern to independent antibody NBP1-85335 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Western-Blot-NBP1-85336-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-85336] - Staining of human cell line CACO-2 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85336-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0013.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0008.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human pancreas shows weak to moderate positivity in luminal membranes in exocrine glandular cells.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0009.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0010.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human liver.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0011.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0012.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human kidney using Anti-VIL1 antibody NBP1-85336.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0014.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining in human placenta and tonsil tissues using anti-LAMC1 antibody. Corresponding LAMC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining in human placenta and tonsil tissues using anti-LAMC1 antibody. Corresponding LAMC1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human cerebral cortex, kidney, placenta and tonsil using Anti-LAMC1 antibody NBP1-87718 (A) shows similar protein distribution across tissues to independent antibody NBP1-87719 (B).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Laminin gamma 1 Antibody [NBP1-87718] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Western-Blot-NBP1-87718-img0014.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human tonsil using Anti-LAMC1 antibody NBP1-87718.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0017.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0008.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0009.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0012.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human kidney.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0013.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody [NBP1-87718] - Staining of human placenta using Anti-LAMC1 antibody NBP1-87718.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87718-img0016.jpg)
![Simple Western: Laminin gamma 1 Antibody [NBP1-87718] - Simple Western lane view shows a specific band for LAMC1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Simple-Western-NBP1-87718-img0005.jpg)
![Simple Western: Laminin gamma 1 Antibody [NBP1-87718] - Electropherogram image(s) of corresponding Simple Western lane view. Laminin gamma 1 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-Simple-Western-NBP1-87718-img0006.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0009.jpg "Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0007.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse trigeminal ganglion shows strong immunoreactivity in ganglion cells.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat brainstem shows strong positivity in neurons of mesencephalic trigeminal nucleus.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse thalamus shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebral cortex shows strong positivity in neural fibers and cell bodies.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Analysis in human endometrium and skeletal muscle tissues using NBP1-85244 antibody. Corresponding MYH9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Analysis in human endometrium and skeletal muscle tissues using NBP1-85244 antibody. Corresponding MYH9 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-MYH9 antibody NBP1-85244 (A) shows similar protein distribution across tissues to independent antibody NBP2-62644 (B).](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85244-img0009.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0017.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0013.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human endometerium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0014.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle fibers and in endothelial cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0015.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining in human testis shows strong cytoplasmic and membranous positivity in Leydig cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0016.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Western-Blot-NBP1-47817-img0013.jpg "Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunocytochemistry-Immunofluorescence-NBP1-47817-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0011.jpg)
![Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Extracts (10ug) from 10 Human tissue by using Cytokeratin 18 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Western-Blot-NBP1-47817-img0014.jpg)
![Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Western-Blot-NBP1-47817-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Western-Blot-NBP1-47817-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0016.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-OTI1E1-Immunohistochemistry-Paraffin-NBP1-47817-img0010.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0007.jpg "Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunofluorescence-NBP2-52433-img0002.jpg)
![Immunohistochemistry: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of paraffin-embedded esophagus tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunohistochemistry-NBP2-52433-img0004.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0005.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunofluorescence-NBP2-52433-img0001.jpg)
![Immunohistochemistry: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of paraffin-embedded esophageal cancer tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunohistochemistry-NBP2-52433-img0003.jpg)
![ELISA: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-ELISA-NBP2-52433-img0008.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0012.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunofluorescence-NBP2-52501-img0011.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0009.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0002.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunocytochemistry-Immunofluorescence-NBP2-52501-img0006.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0008.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0007.jpg)
![ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-ELISA-NBP2-52501-img0004.jpg)
![Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Western-Blot-NB110-96425-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (NB110-96425). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96425) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Immunohistochemistry-NB110-96425-img0007.jpg)
![Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/Laminin-Antibody-LAM-89-Immunohistochemistry-NB600-883-img0006.jpg "Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.")
![Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Analysis in human placenta section with Laminin Antibody (LAM-89) [NB600-883]](http://images.novusbio.com/fullsize/Laminin-Antibody-LAM-89-Immunohistochemistry-NB600-883-img0005.jpg)