| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54503-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-62091SS | |||
| Azide and BSA Free | NBP2-80878 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47946-0.1mg | |||
| IHC-Prediluted | NBP2-48095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-517SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88080-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87334-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84797-25ul | |||
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) [NBP2-53383] - Formalin-fixed, paraffin-embedded human Uterus stained with Desmin Monoclonal Antibody (DES/1711)](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-1711-Immunohistochemistry-Paraffin-NBP2-53383-img0001.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) [NBP2-53383] - Formalin-fixed, paraffin-embedded human Uterus stained with Desmin Monoclonal Antibody (DES/1711)")
![Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate](http://images.novusbio.com/fullsize/NHE8-Antibody-7A11-Western-Blot-NB110-62091-img0003.jpg "Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate")
![Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Western-Blot-NBP2-44827-img0012.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunocytochemistry-Immunofluorescence-NBP2-44827-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0005.jpg "Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.")
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western Blot analysis of total lysates from asynchronous HN30 cells and MCF-12a 3 days post-transfection. NT: non-treated, OB: only buffer, EGFP: only pcDNA3.1/CT-GFP vector, NSP 5a3a/EGFP: pcDNA 3.1/CT-GFP and pcDNA 3.0 NSP 5a3a. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0007.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0003.jpg)
![Immunoprecipitation: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated. NT: non-treated HN30 cells, a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunoprecipitation-NB100-517-img0006.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-517_rabbit-polyclonal-nsp-5-alpha-3-alpha-antibody-310202415525234.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-Western-Blot-NBP2-21604-img0016.jpg "Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE gel.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-Western-Blot-NBP2-21604-img0024.jpg)
![Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-Western-Blot-NBP2-21604-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - Rat esophagus. alpha Tubulin 4a stained by alpha Tubulin 4a antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-Immunohistochemistry-Paraffin-NBP2-21604-img0029.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-21604_rabbit-polyclonal-alpha-tubulin-4a-antibody-910202420115075.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-21604_rabbit-polyclonal-alpha-tubulin-4a-antibody-910202419593537.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-21604_rabbit-polyclonal-alpha-tubulin-4a-antibody-91020242063339.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-21604_rabbit-polyclonal-alpha-tubulin-4a-antibody-91020242011412.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining in human placenta and skeletal muscle tissues . Corresponding KRT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining in human placenta and skeletal muscle tissues . Corresponding KRT7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining of human breast shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0019.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0020.jpg)
![Simple Western: Cytokeratin 7 Antibody [NBP1-88080] - Electropherogram image(s) of corresponding Simple Western lane view. Cytokeratin 7 antibody was used at 1:50 dilution on Tonsil and RT-4 lysate(s).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Simple-Western-NBP1-88080-img0007.jpg)
![Simple Western: Cytokeratin 7 Antibody [NBP1-88080] - Simple Western lane view shows a specific band for KRT7 in 0.2 mg/ml of Tonsil (left), RT-4 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Simple-Western-NBP1-88080-img0006.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0011.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0004.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0005.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0006.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0002.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0003.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Tumorsphere-NBP2-43837-img0019.jpg)
![Immunofluorescence photomicrographs of frozen sections of mouse brain.Green: alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-immunohistochemistry-frozen-2611202518274615.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-western-blot-261120251833101.jpg)
![293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-western-blot-2611202518363315.jpg)
![alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-immunocytochemistry-immunofluorescence-261120251819365.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-western-blot-2611202518443514.jpg)
![alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:250.Red: NeuN, stained by NeuN antibody diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-immunocytochemistry-immunofluorescence-261120251822430.jpg)
![Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NB110-75039-img0001.jpg "Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0002.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87334-img0028.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining in human cerebral cortex and liver tissues . Corresponding UCHL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0027.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0017.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0018.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and in neuropil.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0022.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0023.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0024.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0025.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglion and peripheral nerves.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0026.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84797 (A) shows similar protein distribution across tissues to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis using Anti-PDIA3 antibody NBP1-84797 (A) shows similar pattern to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84797-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84797] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84797-img0007.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0020.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0021.jpg)
