| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54578-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46603SS | |||
| BSA Free | NBP2-46603 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87426-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85335-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).")
![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of PFA-fixed Raji cells. B220/CD45R antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0014.jpg "Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunocytochemistry-Immunofluorescence-H00011004-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunohistochemistry-Paraffin-H00011004-M01-img0010.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi ( Cat # H00011004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01), clone 1G2 (Cat # H00011004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0012.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody (M01), clone 1G2.Lane 1: KIF2C transfected lysate(81.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0015.jpg)
![Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C transfected lysate using anti-KIF2C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KIF2C MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunoprecipitation-H00011004-M01-img0011.jpg)
![Sandwich ELISA: KIF2C Antibody (1G2) [H00011004-M01] - Detection limit for recombinant GST tagged KIF2C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Sandwich-ELISA-H00011004-M01-img0013.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![Western Blot: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Western-Blot-NBP2-80846-img0001.jpg "Western Blot: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi)](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80846-img0003.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80846-img0004.jpg)
![Immunohistochemistry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi)](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80846-img0002.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - Azide and BSA Free [NBP2-80846] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80846-img0005.jpg)
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-132202416173468.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-4320249115619.jpg)
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining in human cerebral cortex and skin tissues using anti-PFN2 antibody. Corresponding PFN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining in human cerebral cortex and skin tissues using anti-PFN2 antibody. Corresponding PFN2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Profilin 2 Antibody [NBP1-87426] - Analysis in human cell lines A-431 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0009.jpg)
![Western Blot: Profilin 2 Antibody [NBP1-87426] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0006.jpg)
![Western Blot: Profilin 2 Antibody [NBP1-87426] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Western-Blot-NBP1-87426-img0007.jpg)
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0010.jpg)
![Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-87426] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Profilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87426-img0011.jpg)
![Western Blot: Villin 1 Antibody [NBP1-85335] - Analysis using Anti-VIL1 antibody NBP1-85335 (A) shows similar pattern to independent antibody NBP1-85336 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Western-Blot-NBP1-85335-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Villin 1 Antibody [NBP1-85335] - Analysis using Anti-VIL1 antibody NBP1-85335 (A) shows similar pattern to independent antibody NBP1-85336 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-85335] - Staining of human cell line CACO-2 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85335-img0002.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85335 (A) shows similar protein distribution across tissues to independent antibody NBP1-85336 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human liver.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0006.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human kidney.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0008.jpg)
![Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Western-Blot-NB300-574-img0009.jpg "Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunocytochemistry-Immunofluorescence-NB300-574-img0005.jpg)
![Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle. Otherwise normal, Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD) muscle biopsy sections were stained for Neu5Gc (green),Spectrin beta 1 (red) and DAPI (blue). Arrows indicate Neu5Gc puncta in cytoplasmic or perimembranous regions of BMD and DMD skeletal myofibers. Bar is 50 um for all panels. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0088226) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunohistochemistry-NB300-574-img0010.jpg)
![Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Flow-Cytometry-NB300-574-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunocytochemistry-Immunofluorescence-NB300-574-img0004.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the membrane of Human skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunohistochemistry-Paraffin-NB300-574-img0006.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm of Human prostate carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunohistochemistry-Paraffin-NB300-574-img0007.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm and membrane of Mouse skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Immunohistochemistry-Paraffin-NB300-574-img0008.jpg)
![Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of SH-SY5Y cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Flow-Cytometry-NB300-574-img0001.jpg)
![Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Spectrin-beta-1-Antibody-4C3-Flow-Cytometry-NB300-574-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle.(A) Otherwise normal, Becker muscular dystrophy (BMD) & Duchenne muscular dystrophy (DMD) muscle biopsy sections were stained for Neu5Gc (green), beta spectrin (red) & DAPI (blue). Arrows indicate Neu5Gc puncta in cytoplasmic or perimembranous regions of BMD & DMD skeletal myofibers. (B) DMD muscle was co-stained for Neu5Gc & CD11b, CD8 or Pax7 (and DAPI). Bar is 50 µm for all panels in A & B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24505439), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-574_mouse-monoclonal-spectrin-beta-1-antibody-4c3-310202415392512.jpg)
![Western Blot: ARPC2 Antibody [NB100-1037] - Staining of CACO-2 with antibody at 0.5 ug/mL (A), HeLa (B) and Jurkat (C) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARPC2-Antibody-Western-Blot-NB100-1037-img0003.jpg "Western Blot: ARPC2 Antibody [NB100-1037] - Staining of CACO-2 with antibody at 0.5 ug/mL (A), HeLa (B) and Jurkat (C) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ARPC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1037-img0005.jpg)
![Immunohistochemistry-Paraffin: ARPC2 Antibody [NB100-1037] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.8 ug/mL.](http://images.novusbio.com/fullsize/ARPC2-Antibody-Immunohistochemistry-Paraffin-NB100-1037-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ARPC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1037-img0004.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NB110-89724-img0018.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0020.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0019.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord (free floating sections) at 1:1000 dilution using Rabbit antibody to c-terminal region of ChAT (choline O-acetyltransferase): whole serum (NB110-89724).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0013.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0012.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0014.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0015.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0016.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human urinary bladder epithelium](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0008.jpg "Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human urinary bladder epithelium")
![Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Flow-Cytometry-NBP1-97730-img0010.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0006.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes and bile ducts](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0007.jpg)
![Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Flow-Cytometry-NBP1-97730-img0009.jpg)