| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44433 | |||
| BSA Free | NBP2-42379-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77333-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37830-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27401SS | |||
| Azide and BSA Free | NBP2-80775 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24591SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44754 | |||
| Azide and BSA Free | NBP2-34670-0.1mg | |||
| Azide and BSA Free | NBP2-34670-0.2mg | |||
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0010.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0017.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0020.jpg)
![Western Blot: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Western blot analysis of ISE6 (Tick, Ixodes scapularis, cell line) lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-Western-Blot-NBP1-77333-img0005.jpg "Western Blot: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Western blot analysis of ISE6 (Tick, Ixodes scapularis, cell line) lysate. WB image submitted by a verified customer review.")
![Western Blot: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-Western-Blot-NBP1-77333-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 4/CD49d Antibody - BSA Free [NBP1-77333] - Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77333_rabbit-polyclonal-integrin-alpha-4-cd49d-antibody-2711202419463422.jpg)
![Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of 20ug of THP-1 whole cell lysate per well onto a SDS-PAGE gel.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Western-Blot-NBP2-37830-img0017.jpg "Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of 20ug of THP-1 whole cell lysate per well onto a SDS-PAGE gel.")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin (green) showing staining in the in the cytoskeleton of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunocytochemistry-Immunofluorescence-NBP2-37830-img0011.jpg)
![Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis showing staining in the cytoskeleton of human colon carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunohistochemistry-Paraffin-NBP2-37830-img0014.jpg)
![Flow (Intracellular): beta Tubulin Antibody (BT7R) [NBP2-37830] - An intracellular stain was performed on HeLa cells with beta Tubulin Antibody (BT7R) NBP2-37830PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Flow-Intracellular-NBP2-37830-img0018.jpg)
![Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of mouse brain tissue lysate loaded at 10 ug/lane and detected with 0.25 ug/ml of NBP2-37830.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Western-Blot-NBP2-37830-img0015.jpg)
![Western Blot: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of 8ug of mouse embryonic stem cell lysate onto an SDS-PAGE gel.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Western-Blot-NBP2-37830-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunocytochemistry-Immunofluorescence-NBP2-37830-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin (green) showing staining in the in the cytoskeleton of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunocytochemistry-Immunofluorescence-NBP2-37830-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin (green) showing staining in the in the cytoskeleton of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Beta-Tubulin loading control antibody in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunocytochemistry-Immunofluorescence-NBP2-37830-img0010.jpg)
![Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta-Tubulin showing staining in the cytoskeleton of paraffin-embedded mouse colon tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta-Tubulin loading control antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunohistochemistry-Paraffin-NBP2-37830-img0012.jpg)
![Immunohistochemistry-Paraffin: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis showing staining in the cytoskeleton of paraffin-embedded human lung adenocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Immunohistochemistry-Paraffin-NBP2-37830-img0013.jpg)
![Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in NIH-3T3 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Flow-Cytometry-NBP2-37830-img0006.jpg)
![Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Flow-Cytometry-NBP2-37830-img0007.jpg)
![Flow Cytometry: beta Tubulin Antibody (BT7R) [NBP2-37830] - Analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-BT7R-Flow-Cytometry-NBP2-37830-img0008.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0005.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1005-img0001.jpg)
![Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NB100-1005-img0002.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0003.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue. Cells were probed without (control) or with or an antibody recognizing Vimentin](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Western-Blot-NB100-74564-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo")
![Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-NBP1-97715-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0005.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0002.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0001.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein on ( A) Full-length human HMGB1 protein (B) human Jurkat cell lysate and (C) mouse NIH 3T3 cell lysate using HMGB1 antibody (clone 19N15F4) at 2 ug/ml concentration. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used in this assay.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Western-Blot-NBP2-27401-img0026.jpg "Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein on ( A) Full-length human HMGB1 protein (B) human Jurkat cell lysate and (C) mouse NIH 3T3 cell lysate using HMGB1 antibody (clone 19N15F4) at 2 ug/ml concentration. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used in this assay.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - IHC-P detection of HMGB1 protein in formalin-fixed, decalcified, paraffin embedded tissue sections from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml [Image courtesy of Dr Ulf Andersson, Karolinska]](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0027.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Flow-Cytometry-NBP2-27401-img0016.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Formalin-fixed, paraffin-embedded human skin stained with HMGB1 antibody at 5 ug/ml](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0018.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of human breast normal tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The ductal /acinar epithelial cells in the breast tissue section depicted strong nuclear expression with some cytoplasmic positivity. The myoepithelial cells and few cells of the intra-lobular connective tissue showed relatively weak nuclear positivity for HMGB1.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0019.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of normal human stomach tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The cells of the glandular stomach showed specific nuclear expression for HMGB1.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0020.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of normal human colon using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Almost all the cells of colon's mucosal layer showed nuclear positivity for HMGB1 but the cells of the columnar epithelial cells on the absorptive surface developed cytoplasmic staining also.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0021.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of normal human brain using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The representative image shows an overall strong nuclear HMGB1 immunopositivity with weak to negligible cytoplasmic staining in brain cells.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0022.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of human bladder transitional cell carcinoma (TCC) / urothelial cell carcinoma (UCC) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Almost all the cancer cells and the cells of tumor stroma developed strong/specific nuclear HMGB1 immunopositivity.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0023.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of human esophageal squamous cell carcinoma (SCC) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Strong nuclear along with weak cytoplasmic immunopositivity for HMGB1 was observed in SCC cells and the tumor stroma cells developed relatively weak staining for this target.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0024.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of human small intestinal cancer tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Intense nuclear immunopositivity of HMGB1 was observed in cancer cells and the cells of tumor stroma as well as the adjacent normal crypts. Some glandular cells in the crypts developed bother nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N15F4-Immunohistochemistry-Paraffin-NBP2-27401-img0025.jpg)
![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0024.jpg "Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibodyused for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30475] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP2-76410 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30475-img0022.jpg)
![Western Blot: Rab7a Antibody [NBP2-24591] - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Western-Blot-NBP2-24591-img0004.jpg "Western Blot: Rab7a Antibody [NBP2-24591] - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24591-img0006.jpg)
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-24591] - Analysis of human skeletal muscle using RAB7A antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP2-24591-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24591-img0005.jpg)
![Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Western-Blot-NBP2-32973-img0010.jpg "Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973] - Immunofluorescent staining of PFA-fixed MCF-7 cells. followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunocytochemistry-Immunofluorescence-NBP2-32973-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP60 MAb (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0001.jpg)
![Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using HSP60 antibody (LK1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Flow-Cytometry-NBP2-32973-img0011.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (LK1).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0005.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0002.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0004.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP1-31302-img0008.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0010.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0011.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0012.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0025.jpg "Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0026.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0020.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0016.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0017.jpg)
![Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NBP1-32075-img0023.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32075_rabbit-polyclonal-pcna-antibody-910202420131282.jpg)
